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Western Blot (WB) (Western BlotPositive WB detected in: 293 whole cell lysateAll lanes: HIST1H2BC antibody at 0.47ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 14 KDaObserved band size: 14 KDa)

Rabbit anti-Human HIST1H2BC Polyclonal Antibody | anti-HIST1H2BC antibody

HIST1H2BC(Ab-85) Antibody

Gene Names
HIST1H2BG; H2B/a; H2BFA; H2B.1A; dJ221C16.8
Reactivity
Human
Applications
ELISA, Western Blot, Immunofluorescence
Purity
Antigen Affinity Purified
Synonyms
HIST1H2BC; Polyclonal Antibody; HIST1H2BC(Ab-85) Antibody; Histone H2B type 1-C/E/F/G/I; Histone H2B.1 A; Histone H2B.a; H2B/a; Histone H2B.g; H2B/g; Histone H2B.h; H2B/h; Histone H2B.k; H2B/k; Histone H2B.l; H2B/l; H2BFL; AND; HIST1H2BE; H2BFH; HIST1H2BF; H2BFG; HIST1H2BG; H2BFA; HIST1H2BI; H2BFK; anti-HIST1H2BC antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid
Sequence Length
126
Applicable Applications for anti-HIST1H2BC antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:5000
IF: 1:50-1:200
Immunogen
Peptide sequence around site of beta?hydroxybutyryl-Lys(85) derived from human Histone H2B
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: 293 whole cell lysateAll lanes: HIST1H2BC antibody at 0.47ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 14 KDaObserved band size: 14 KDa)

Western Blot (WB) (Western BlotPositive WB detected in: 293 whole cell lysateAll lanes: HIST1H2BC antibody at 0.47ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 14 KDaObserved band size: 14 KDa)

Immunofluorescence (IF)

(Immunofluorescence staining of Hela cells with MBS7110598 at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

Immunofluorescence (IF) (Immunofluorescence staining of Hela cells with MBS7110598 at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Product Categories/Family for anti-HIST1H2BC antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
13,906 Da
NCBI Official Full Name
histone H2B type 1-C/E/F/G/I
NCBI Official Synonym Full Names
histone cluster 1 H2B family member g
NCBI Official Symbol
HIST1H2BG
NCBI Official Synonym Symbols
H2B/a; H2BFA; H2B.1A; dJ221C16.8
NCBI Protein Information
histone H2B type 1-C/E/F/G/I
UniProt Protein Name
Histone H2B type 1-C/E/F/G/I
Protein Family
UniProt Gene Name
HIST1H2BC
UniProt Synonym Gene Names
H2BFL; H2B/a; H2B/g; H2B/h; H2B/k; H2B/l

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein has antibacterial and antifungal antimicrobial activity. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

Uniprot Description

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Research Articles on HIST1H2BC

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Product Notes

The HIST1H2BC hist1h2bc (Catalog #AAA7110598) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HIST1H2BC(Ab-85) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HIST1H2BC can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:5000 IF: 1:50-1:200. Researchers should empirically determine the suitability of the HIST1H2BC hist1h2bc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIST1H2BC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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