Western Blot (WB)
(HIST1H2BA Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the HIST1H2BA Antibody detected the HIST1H2BA protein (arrow).)
Rabbit anti-Human HIST1H2BA Polyclonal Antibody | anti-HIST1H2BA antibody
HIST1H2BA, NT (HIST1H2BA, TSH2B, Histone H2B type 1-A, Histone H2B, testis, Testis-specific histone H2B) (Azide free) (HRP)
Gene Names
HIST1H2BA; STBP; H2BFU; TSH2B; bA317E16.3
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
HIST1H2BA; Polyclonal Antibody; NT (HIST1H2BA; TSH2B; Histone H2B type 1-A; Histone H2B; testis; Testis-specific histone H2B) (Azide free) (HRP); Testis-specific histone H2B; anti-HIST1H2BA antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-HIST1H2BA antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
HIST1H2BA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HIST1H2BA.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(HIST1H2BA Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the HIST1H2BA Antibody detected the HIST1H2BA protein (arrow).)
Western Blot (WB)
(HIST1H2BA Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the HIST1H2BA Antibody detected the HIST1H2BA protein (arrow).)
Related Product Information for anti-HIST1H2BA antibody
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a testis/sperm-specific member of the histone H2B family. Transcripts from this gene contain a palindromic termination element. [provided by RefSeq].
Product Categories/Family for anti-HIST1H2BA antibody
References
Kim, S.C., et al. Mol. Cell 23(4):607-618(2006). Pavri, R., et al. Cell 125(4):703-717(2006). Zhu, B., et al. Mol. Cell 20(4):601-611(2005). Golebiowski, F., et al. Mol. Cell. Biochem. 279 (1-2), 133-139 (2005): Li, A., et al. Biochemistry 44(7):2529-2535(2005).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
127
NCBI Official Full Name
histone H2B type 1-A
NCBI Official Synonym Full Names
histone cluster 1, H2ba
NCBI Official Symbol
HIST1H2BA
NCBI Official Synonym Symbols
STBP; H2BFU; TSH2B; bA317E16.3
NCBI Protein Information
histone H2B type 1-A; histone 1, H2ba; histone H2B, testis; testis-specific histone H2B; H2B histone family, member U, (testis-specific)
UniProt Protein Name
Histone H2B type 1-A
Protein Family
UniProt Gene Name
HIST1H2BA
UniProt Synonym Gene Names
TSH2B
UniProt Entry Name
H2B1A_HUMAN
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Product Notes
The HIST1H2BA hist1h2ba (Catalog #AAA6306784) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HIST1H2BA, NT (HIST1H2BA, TSH2B, Histone H2B type 1-A, Histone H2B, testis, Testis-specific histone H2B) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HIST1H2BA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HIST1H2BA hist1h2ba for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIST1H2BA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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