Rabbit anti-Human HIST1H1D Polyclonal Antibody | anti-HIST1H1D antibody

HIST1H1D, ID (HIST1H1D, H1F3, Histone H1.3, Histone H1c, Histone H1s-2) (PE)

Cat. Num. AAA6306702

• Gene Names: HIST1H1D; H1D; H1.3; H1F3; H1s-2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HIST1H1D; Polyclonal Antibody; ID (HIST1H1D; H1F3; Histone H1.3; Histone H1c; Histone H1s-2) (PE); Histone H1s-2; anti-HIST1H1D antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-HIST1H1D antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: HIST1H1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-164 amino acids from the Central region of human HIST1H1D.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(HIST1H1D Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HIST1H1D antibody detected the HIST1H1D protein (arrow).)

Immunohistochemistry (IHC)

(HIST1H1D Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HIST1H1D Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-HIST1H1D antibody

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

Product Categories/Family for anti-HIST1H1D antibody

Antibodies; Histones

References

Kim, J.J., et al. J. Hum. Genet. 55(1):27-31(2010). Soranzo, N., et al. PLoS Genet. 5 (4), E1000445 (2009): Sovio, U., et al. PLoS Genet. 5 (3), E1000409 (2009): Gudbjartsson, D.F., et al. Nat. Genet. 40(5):609-615(2008). Lettre, G., et al. Nat. Genet. 40(5):584-591(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4885377
• NCBI GeneID: 3007
• NCBI Accession #: NP_005311.1
• NCBI GenBank Nucleotide #: NM_005320.2
• UniProt Accession #: P16402; Q2M2I2; B2R751
• Molecular Weight: 22,350 Da
• NCBI Official Full Name: histone H1.3
• NCBI Official Synonym Full Names: histone cluster 1 H1 family member d
• NCBI Official Symbol: HIST1H1D
• NCBI Official Synonym Symbols: H1D; H1.3; H1F3; H1s-2
• NCBI Protein Information: histone H1.3
• UniProt Protein Name: Histone H1.3
• UniProt Gene Name: HIST1H1D
• UniProt Synonym Gene Names: H1F3

NCBI Description

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]

Uniprot Description

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation ().