Rabbit anti-Human His6-DEP-1 Polyclonal Antibody | anti-His6-DEP-1 antibody

His6-DEP-1 (PTPRJ, DEP1, Receptor-type tyrosine-protein phosphatase eta, Density-enhanced phosphatase 1, HPTP eta, Protein-tyrosine phosphatase receptor type J, CD148) (Azide free) (HRP)

Cat. Num. AAA6306663

• Gene Names: PTPRJ; DEP1; SCC1; CD148; HPTPeta; R-PTP-ETA
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: His6-DEP-1; Polyclonal Antibody; His6-DEP-1 (PTPRJ; DEP1; Receptor-type tyrosine-protein phosphatase eta; Density-enhanced phosphatase 1; HPTP eta; Protein-tyrosine phosphatase receptor type J; CD148) (Azide free) (HRP); PTPRJ; CD148; anti-His6-DEP-1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-His6-DEP-1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: Poly-HIS protein
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(His6-DEP-1 western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the DEP-1 antibody detected the DEP-1 protein (arrow).)

Immunohistochemistry (IHC)

(His6-DEP-1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of His6-DEP-1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of His6-DEP-1 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-His6-DEP-1 antibody

May contribute to the mechanism of contact inhibition of cell growth.

Product Categories/Family for anti-His6-DEP-1 antibody

Antibodies; CD Markers

NCBI and Uniprot Product Information

• NCBI GI #: 148728162
• NCBI GeneID: 5795
• NCBI Accession #: NP_002834.3
• NCBI GenBank Nucleotide #: NM_002843.3
• UniProt Accession #: Q12913; Q15255; Q6P4H4; Q8NHM2; Q9UDA9
• Molecular Weight: 57,190 Da
• NCBI Official Full Name: receptor-type tyrosine-protein phosphatase eta isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type, J
• NCBI Official Symbol: PTPRJ
• NCBI Official Synonym Symbols: DEP1; SCC1; CD148; HPTPeta; R-PTP-ETA
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase eta; DEP-1; R-PTP-J; HPTP eta; CD148 antigen; density-enhanced phosphatase 1; protein-tyrosine phosphatase eta; human density enhanced phosphatase-1; protein-tyrosine phosphatase receptor type J; susceptibility t
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase eta
• UniProt Gene Name: PTPRJ
• UniProt Synonym Gene Names: DEP1; Protein-tyrosine phosphatase eta; R-PTP-eta; DEP-1; R-PTP-J

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. This protein is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 and Linker for Activation of T Cells. This protein can also dephosphorylate the PDGF beta receptor, and may be involved in UV-induced signal transduction. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, FLT3, PDGFRB, MET, RET (variant MEN2A), KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Involved in vascular development. Regulator of macrophage adhesion and spreading. Positively affects cell-matrix adhesion. Positive regulator of platelet activation and thrombosis. Negative regulator of cell proliferation. Negative regulator of PDGF-stimulated cell migration; through dephosphorylation of PDGFR. Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation; through KDR dephosphorylation. Negative regulator of EGFR signaling pathway; through EGFR dephosphorylation. Enhances the barrier function of epithelial junctions during reassembly. Negatively regulates T-cell receptor (TCR) signaling. Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling.