Rabbit HIRA Polyclonal Antibody | anti-HIRA antibody

HIRA Antibody

Cat. Num. AAA9612232

• Gene Names: HIRA; TUP1; DGCR1; TUPLE1
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: HIRA; Polyclonal Antibody; HIRA Antibody; DGCR1; DiGeorge critical region gene 1; HIR; HIR histone cell cycle regulation defective homolog A; HIRA protein; HIRA_HUMAN; Histone cell cycle regulator; Protein HIRA; TUP 1; TUP1 like enhancer of split protein 1; TUP1-like enhancer of split protein 1; TUPLE1; anti-HIRA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: HIRA Antibody detects endogenous levels of total HIRA.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1017

• Applicable Applications for anti-HIRA antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human HIRA, corresponding to a region within N-terminal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells, using HIRA Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-HIRA antibody

Function: Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.

Post Translational Modifications: Sumoylated.Phosphorylated by CDK2/CCNA1 and CDK2/CCNE1 on Thr-555 in vitro. Also phosphorylated on Thr-555 and Ser-687 in vivo.

Subcellular Location: Nucleus. Nucleus>PML body. Note: Primarily, though not exclusively, localized to the nucleus. Localizes to PML bodies immediately prior to onset of senescence.

Tissue Specificity: Expressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart, liver, lung, and placenta.

Subunit Structure: Interacts with histone H3-3B, PAX3 and PAX7 (By similarity). Interacts with histone H3.Y (PubMed:28334823). Interacts with CCNA1, HIRIP3, NFU1/HIRIP5 and histone H2B. Part of a complex which includes ASF1A, CABIN1, histone H3.3, histone H4 and UBN1 (PubMed:11238922, PubMed:14680630, PubMed:14718166, PubMed:15621527, PubMed:16980972, PubMed:19029251, PubMed:9710638).

Similarity: Belongs to the WD repeat HIR1 family.

NCBI and Uniprot Product Information

• NCBI GI #: 21536485
• NCBI GeneID: 7290
• NCBI Accession #: NP_003316.3
• NCBI GenBank Nucleotide #: NP_003316.3
• UniProt Accession #: P54198
• Molecular Weight: Observed Molecular Weight: (Observed)111kD.; Predicted Molecular Weight: (Calculated)112kDa.
• NCBI Official Full Name: protein HIRA
• NCBI Official Synonym Full Names: histone cell cycle regulator
• NCBI Official Symbol: HIRA
• NCBI Official Synonym Symbols: TUP1; DGCR1; TUPLE1
• NCBI Protein Information: protein HIRA
• UniProt Protein Name: Protein HIRA
• Protein Family: HIRA-interacting protein
• UniProt Gene Name: HIRA
• UniProt Synonym Gene Names: DGCR1; HIR; TUPLE1
• UniProt Entry Name: HIRA_HUMAN

NCBI Description

This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development. [provided by RefSeq, Jul 2008]

Uniprot Description

HIRA: a histone chaperone. Necessary to mediate DNA-synthesis-independent nucleosome assembly. Interacts with UBN1, CABIN, and ASF1A in the cell nucleus to form the evolutionarily conserved HUCA histone chaperone complex that deposits the variant histone H3.3 into chromatin in a DNA-replication independent manner. Required for deposition of histone H3.3 at the transcription start sites of genes, where incorporation of histone H3.3 facilitates nucleosome destabilization and contributes to transcriptional activation. Histone H3.3 is also linked to gene silencing and is incorporated into regions of the genome thought to be transcriptionally inactive. While some incorporation of H3.3 into heterochromatin is facilitated by an additional histone chaperone complex containing ATRX and DAXX (ie. telomeric incorporation of H3.3), HIRA is required for incorporation of histone H3.3 and formation of senescence-associated heterochromatin foci (SAHF) during cellular senescence. HIRA is ubiquitously expressed during mouse embryonic development. In the adult mouse, HIRA is expressed at high levels in the kidney, skeletal muscle, and pancreas, but it is expressed at lower levels in the heart, lung, placenta, brain, and liver. A missing copy of the HIRA gene on human chromosome region 22q11.2 is a common characteristic of DiGeorge syndrome patients and insufficient production of the HIRA protein may disrupt normal embryonic development. Two isoforms of the human protein result from alternative splicing.

Protein type: Cell development/differentiation; Transcription regulation; Cell cycle regulation

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: nucleoplasm; PML body; protein complex; nuclear chromatin; nucleus

Molecular Function: protein binding; chromatin binding; transcription corepressor activity; transcription factor activity

Biological Process: osteoblast differentiation; regulation of transcription from RNA polymerase II promoter; anatomical structure morphogenesis; muscle cell differentiation; transcription, DNA-dependent; DNA replication-independent nucleosome assembly; gastrulation; chromatin modification