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Western Blot (WB) (Western Blot: Sample: Human Jurkat cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human HMG20B Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Rabbit anti-Human High Mobility Group Protein 20B Polyclonal Antibody | anti-HMG20B antibody

Polyclonal Antibody to High Mobility Group Protein 20B (HMG20B)

Gene Names
HMG20B; SOXL; HMGX2; BRAF25; BRAF35; HMGXB2; PP7706; pp8857; SMARCE1r
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
High Mobility Group Protein 20B; Polyclonal Antibody; Polyclonal Antibody to High Mobility Group Protein 20B (HMG20B); BRAF35; HMGX2; SMARCE1r; SOXL; Sox-like transcriptional factor; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related; anti-HMG20B antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against HMG20B. It has been selected for its ability to recognize HMG20B in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, Containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence Length
319
Applicable Applications for anti-HMG20B antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Fragment
HMG20B (Val27~Phe213)
Conjugate
No Conjugate
Cross Reactivity
Human
Immunoglobulin Type
IgG
Quality Control
Content: The quality control contains recombinant HMG20B (Val27~Phe213) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2072840
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Human Jurkat cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human HMG20B Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Human Jurkat cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human HMG20B Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded stomach tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded stomach tissue))

Knockout Validation

(Knockout Validation: Lane 1: Wild-type Jurkat cell lysate; Lane 2: HMG20B knockout Jurkat cell lysate; Predicted MW: 25~36kd Observed MW: 40kd Primary Ab: 1ug/ml Rabbit Anti-Human HMG20B Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))

Knockout Validation (Knockout Validation: Lane 1: Wild-type Jurkat cell lysate; Lane 2: HMG20B knockout Jurkat cell lysate; Predicted MW: 25~36kd Observed MW: 40kd Primary Ab: 1ug/ml Rabbit Anti-Human HMG20B Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Product Categories/Family for anti-HMG20B antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32,939 Da
NCBI Official Full Name
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related
NCBI Official Synonym Full Names
high mobility group 20B
NCBI Official Symbol
HMG20B
NCBI Official Synonym Symbols
SOXL; HMGX2; BRAF25; BRAF35; HMGXB2; PP7706; pp8857; SMARCE1r
NCBI Protein Information
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related
UniProt Protein Name
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related
UniProt Gene Name
HMG20B
UniProt Synonym Gene Names
BRAF35; HMGX2; HMGXB2; SMARCE1R; SMARCE1-related protein

Uniprot Description

HMG20B: Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: chromosome; nuclear body; nucleoplasm

Molecular Function: DNA binding; histone deacetylase activity; protein binding; protein heterodimerization activity

Biological Process: blood coagulation; cell cycle; histone deacetylation; negative regulation of protein sumoylation; positive regulation of neuron differentiation; regulation of transcription, DNA-templated; transcription, DNA-dependent

Research Articles on HMG20B

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Product Notes

The HMG20B hmg20b (Catalog #AAA2033874) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to High Mobility Group Protein 20B (HMG20B) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's High Mobility Group Protein 20B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-1:5000. Researchers should empirically determine the suitability of the HMG20B hmg20b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "High Mobility Group Protein 20B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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