Rabbit anti-Human, Mouse HGS Polyclonal Antibody | anti-HGS antibody

HGS, NT (HRS, Hepatocyte Growth Factor Regulated Tyrosine Kinase Substrate, HGNC:4897, Human Growth Factor Regulated Tyrosine Kinase Substrate, Protein pp110, ZFYVE8) (FITC)

Cat. Num. AAA6481176

• Gene Names: HGS; HRS
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: HGS; Polyclonal Antibody; NT (HRS; Hepatocyte Growth Factor Regulated Tyrosine Kinase Substrate; HGNC:4897; Human Growth Factor Regulated Tyrosine Kinase Substrate; Protein pp110; ZFYVE8) (FITC); anti-HGS antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HGS. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-HGS antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human HGS (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of hIMOS-1-Q271 in mouse spleen tissue lysates (35ug/lane). IMOS-1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-HGS antibody

Antibodies; Transcription Factors

References

1. Kirk E, et al. J Cell Sci. 2006 Nov 15;119(Pt 22):4689-701 2. Sasaki Y, et al. J Biol Chem. 2001 Aug 10;276(32):29943-52. 3. Miura S, et al. Mol Cell Biol. 2000 Dec;20(24):9346-55.

NCBI and Uniprot Product Information

• NCBI GI #: 4758528
• NCBI GeneID: 9146
• NCBI Accession #: NP_004703
• NCBI GenBank Nucleotide #: NM_004712
• UniProt Accession #: O14964; Q9NR36
• Molecular Weight: 76,376 Da
• NCBI Official Full Name: hepatocyte growth factor-regulated tyrosine kinase substrate
• NCBI Official Synonym Full Names: hepatocyte growth factor-regulated tyrosine kinase substrate
• NCBI Official Symbol: HGS
• NCBI Official Synonym Symbols: HRS
• NCBI Protein Information: hepatocyte growth factor-regulated tyrosine kinase substrate; human growth factor-regulated tyrosine kinase substrate; protein pp110
• UniProt Protein Name: Hepatocyte growth factor-regulated tyrosine kinase substrate
• Protein Family: Hepatocyte growth factor-regulated tyrosine kinase substrate
• UniProt Gene Name: HGS
• UniProt Synonym Gene Names: HRS
• UniProt Entry Name: HGS_HUMAN

Uniprot Description

Hrs: a zinc finger protein which is rapidly tyrosine phosphorylated in cells stimulated with various growth factors. Hrs mRNA is expressed in all the human adult and fetal tissues tested.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: intracellular membrane-bound organelle; early endosome membrane; early endosome; cytoplasm; cytosol; endosome; secretory granule

Molecular Function: protein domain specific binding; protein binding; metal ion binding

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; epidermal growth factor receptor signaling pathway; negative regulation of cell proliferation; protein targeting to lysosome; membrane invagination; endosome to lysosome transport; regulation of protein catabolic process; endosome transport; signal transduction; negative regulation of JAK-STAT cascade