Rabbit anti-Human HGF Polyclonal Antibody | anti-HGF antibody

HGF, CT (Hepatocyte Growth Factor, Scatter Factor, SF, Hepatopoeitin A, HGF, HPTA) (MaxLight 650)

Cat. Num. AAA6479999

• Gene Names: HGF; SF; HGFB; HPTA; F-TCF; DFNB39
• Reactivity: Human
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: HGF; Polyclonal Antibody; CT (Hepatocyte Growth Factor; Scatter Factor; SF; Hepatopoeitin A; HPTA) (MaxLight 650); anti-HGF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HGF.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-HGF antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Flow Cytometry (FC/FACS), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 521-554 in the C-terminal region of human HGF.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot to detect HGF (arrow) in Ramos tissue lysate using H2005-08A.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with H2005-08A, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of H2005-08A using CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-HGF antibody

Antibodies; Growth Factors; Cytokines

References

1. Ryugo, M., et al., Transplantation 78(8):1153-1158 (2004). 2. Lyon, M., et al., J. Biol. Chem. 279(42):43560-43567 (2004). 3. He, Y., et al., World J. Gastroenterol. 10(19):2827-2830 (2004). 4. Tjin, E.P., et al., Blood 104(7):2172-2175 (2004). 5. Matsuda-Hashii, Y., et al., Exp. Hematol. 32(10):955-961 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 33859835
• NCBI GeneID: 3082
• NCBI Accession #: NP_000592
• NCBI GenBank Nucleotide #: NM_000601
• UniProt Accession #: P14210; Q02935; Q13494; Q14519; Q3KRB2; Q8TCE2; Q9BYL9; Q9BYM0; Q9UDU6; A1L3U6
• Molecular Weight: 83,134 Da
• NCBI Official Full Name: hepatocyte growth factor isoform 1 preproprotein
• NCBI Official Synonym Full Names: hepatocyte growth factor (hepapoietin A; scatter factor)
• NCBI Official Symbol: HGF
• NCBI Official Synonym Symbols: SF; HGFB; HPTA; F-TCF; DFNB39
• NCBI Protein Information: hepatocyte growth factor; hepatopoeitin-A; hepatopoietin-A; lung fibroblast-derived mitogen; fibroblast-derived tumor cytotoxic factor
• UniProt Protein Name: Hepatocyte growth factor
• Protein Family: Hepatocyte growth factor
• UniProt Gene Name: HGF
• UniProt Synonym Gene Names: HPTA; SF
• UniProt Entry Name: HGF_HUMAN

NCBI Description

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

HGF: HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. It has no detectable protease activity. Activating ligand for the receptor tyrosine kinase MET by binding and promoting its dimerization. Defects in HGF are the cause of deafness autosomal recessive type 39 (DFNB39). A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Belongs to the peptidase S1 family. Plasminogen subfamily. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Hormone; Cell development/differentiation

Chromosomal Location of Human Ortholog: 7q21.1

Cellular Component: extracellular space; membrane; extracellular region

Molecular Function: identical protein binding; protein binding; growth factor activity; protein heterodimerization activity; serine-type endopeptidase activity; chemoattractant activity

Biological Process: positive regulation of myelination; mitosis; platelet activation; organ regeneration; activation of MAPK activity; myoblast proliferation; hepatocyte growth factor receptor signaling pathway; negative regulation of caspase activity; proteolysis; liver development; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of osteoblast differentiation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of angiogenesis; platelet degranulation; positive chemotaxis; epithelial to mesenchymal transition; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; hyaluronan metabolic process; positive regulation of cell migration

Disease: Deafness, Autosomal Recessive 39