Rabbit anti-Human HERC5 Polyclonal Antibody | anti-HERC5 antibody

Anti-HERC5 Antibody

Cat. Num. AAA1752517

• Gene Names: HERC5; CEB1; CEBP1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Immunogen Affinity Purified
• Synonyms: HERC5; Polyclonal Antibody; Anti-HERC5 Antibody; E3 ISG15--protein ligase HERC5; HECT and RLD domain containing E3 ubiquitin protein ligase 5; Cyclin-E-binding protein 1; HECT domain and RCC1-like domain-containing protein 5; CEB1; CEBP1; anti-HERC5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized
• Sequence Length: 1024

• Applicable Applications for anti-HERC5 antibody: Western Blot (WB), Direct ELISA (EIA)
• Application Notes: WB: 0.1-0.5ug/ml; Direct ELISA: 0.1-0.5ug/ml
• Immunogen: E Coli-derived human HERC5 recombinant protein (Position: F793-G1024).
• Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Relevant Detection Systems: Relevant Detection Systems
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500mug/ml.
• Relevant Detection Systems: Relevant Detection Systems
• Relevant Detection Systems: We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Related Product Information for anti-HERC5 antibody

Description: Rabbit IgG polyclonal antibody for HERC5 detection. Tested with WB, Direct ELISA in Human.

Background: Probable E3 ubiquitin-protein ligase HERC5 is an enzyme that in humans is encoded by the HERC5 gene. It is mapped to 4q22.1. This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets.

References

1. Hochrainer, K., Mayer, H., Baranyi, U., Binder, B. R., Lipp, J., Kroismayr, R. The human HERC family of ubiquitin ligases: novel members, genomic organization, expression profiling, and evolutionary aspects. Genomics 85: 153-164, 2005. 2. Mitsui, K., Nakanishi, M., Ohtsuka, S., Norwood, T. H., Okabayashi, K., Miyamoto, C., Tanaka, K., Yoshimura, A., Ohtsubo, M. A novel human gene encoding HECT domain and RCC1-like repeats interacts with cyclins and is potentially regulated by the tumor suppressor proteins. Biochem. Biophys. Res. Commun. 266: 115-122, 1999. 3. Wong, J. J. Y., Pung, Y. F., Sze, N. S.-K., Chin, K.-C. HERC5 is an IFN-induced HECT-type E3 protein ligase that mediates type I IFN-induced ISGylation of protein targets. Proc. Nat. Acad. Sci. 103: 10735-10740, 2006.

NCBI and Uniprot Product Information

• NCBI GI #: 110825982
• NCBI GeneID: 51191
• NCBI Accession #: NP_057407
• NCBI GenBank Nucleotide #: NP_057407.2
• UniProt Accession #: Q9UII4
• NCBI Official Full Name: E3 ISG15--protein ligase HERC5
• NCBI Official Synonym Full Names: HECT and RLD domain containing E3 ubiquitin protein ligase 5
• NCBI Official Symbol: HERC5
• NCBI Official Synonym Symbols: CEB1; CEBP1
• NCBI Protein Information: E3 ISG15--protein ligase HERC5
• UniProt Protein Name: E3 ISG15--protein ligase HERC5
• Protein Family: E3 ISG15--protein ligase
• UniProt Gene Name: HERC5
• UniProt Synonym Gene Names: CEB1; CEBP1

NCBI Description

This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4. [provided by RefSeq, Jul 2008]

Uniprot Description

Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon. Functions as part of the ISGylation machinery that recognizes target proteins in a broad and relatively non-specific manner. Catalyzes ISGylation of IRF3 which results in sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response. Catalyzes ISGylation of influenza A viral NS1 which attenuates virulence; ISGylated NS1 fails to form homodimers and thus to interact with its RNA targets. Catalyzes ISGylation of papillomavirus type 16 L1 protein which results in dominant-negative effect on virus infectivity. Physically associated with polyribosomes, broadly modifies newly synthesized proteins in a cotranslational manner. In an interferon-stimulated cell, newly translated viral proteins are primary targets of ISG15.