Rabbit HERC2 Polyclonal Antibody | anti-HERC2 antibody
HERC2 Antibody
Gene Names
HERC2; jdf2; p528; MRT38; SHEP1; D15F37S1
Reactivity
Human, Mouse, Rat
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
HERC2; Polyclonal Antibody; HERC2 Antibody; D15F37S1; E3 ubiquitin-protein ligase HERC2; HECT and RLD domain containing E3 ubiquitin protein ligase 2; HECT domain and RCC1 like domain containing protein 2; HECT domain and RCC1-like domain-containing protein 2; Hect domain and RLD 2; HERC2_HUMAN; jdf2; MRT38; p528; Probable E3 ubiquitin-protein ligase HERC2; SHEP1; anti-HERC2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
HERC2 Antibody detects endogenous levels of total HERC2.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-HERC2 antibody
ELISA (EIA)(peptide)
Application Notes
ELISA(peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from Human MYOCD.
Conjugation
Unconjugated
Similarity
The ZZ-type zinc finger mediates binding to SUMO1, and at low level SUMO2. The RCC1 repeats are grouped into three seven-bladed beta-propeller regions.
Post Translational Modifications
Phosphorylation at Thr-4827 is required for interaction with RNF8.Sumoylated with SUMO1 by PIAS4 in response to double-strand breaks (DSBs), promoting the interaction with RNF8.
Subunit Structure
Interacts (when phosphorylated at Thr-4827 and sumoylated) with RNF8 (via FHA domain); this interaction increases after ionizing radiation (IR) treatment. Interacts with XPA. Interacts with NEURL4. Via its interaction with NEURL4, may indirectly interact with CCP110 and CEP97.
Function
E3 ubiquitin-protein ligase that regulates ubiquitin-dependent retention of repair proteins on damaged chromosomes. Recruited to sites of DNA damage in response to ionizing radiation (IR) and facilitates the assembly of UBE2N and RNF8 promoting DNA damage-induced formation of 'Lys-63'-linked ubiquitin chains. Acts as a mediator of binding specificity between UBE2N and RNF8. Involved in the maintenance of RNF168 levels. E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of XPA which influences the circadian oscillation of DNA excision repair activity. By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway.
Note
Recruited to sites of DNA damage in response to ionizing radiation (IR) via its interaction with RNF8. May loose association with centrosomes during mitosis.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Product Categories/Family for anti-HERC2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
527,228 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase HERC2
NCBI Official Synonym Full Names
HECT and RLD domain containing E3 ubiquitin protein ligase 2
NCBI Official Symbol
HERC2
NCBI Official Synonym Symbols
jdf2; p528; MRT38; SHEP1; D15F37S1
NCBI Protein Information
E3 ubiquitin-protein ligase HERC2
UniProt Protein Name
E3 ubiquitin-protein ligase HERC2
Protein Family
UniProt Gene Name
HERC2
UniProt Entry Name
HERC2_HUMAN
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Product Notes
The HERC2 herc2 (Catalog #AAA9629399) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HERC2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HERC2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA)(peptide). ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the HERC2 herc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HERC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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