Rabbit anti-Mouse Hephaestin Polyclonal Antibody | anti-HEPH antibody

Hephaestin (Hp)

Cat. Num. AAA617961

• Gene Names: HEPH; CPL; KIAA0698
• Reactivity: Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Hephaestin; Polyclonal Antibody; Hephaestin (Hp); Anti -Hephaestin (Hp); anti-HEPH antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Hephaestin.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.

• Applicable Applications for anti-HEPH antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1-10ug/ml using ECL.; ELISA: 1:100,000 using 50-100ng H2030-05 Control peptide/well.; Immunohistochemistry: See citation
• Immunogen: A synthetic peptide corresponding to 18-aa sequence within the C-terminus of mouse/rat Hp (KLH). Species sequence homology: rat- 100%, human 94%.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-HEPH antibody

Elemental iron is required for a variety of normal cellular functions and vital for proper growth and development. However, natural iron is quite insoluble and excess iron is harmful, since it can catalyze the formation of potentially damaging reactive oxygen species. Humans also have very limited capacity to excrete iron. Therefore, cells have developed mechanisms to improve solubility of iron and to control intracellular iron levels at the point of absorption in the intestine and other tissue. Several proteins including Ferritin, transferrin (Tf), transferrin receptors (TfRs), and iron regulatory proteins (IRPs), iron transporter (NRMAP2/DMT1/DCT1) etc play a key role in iron metabolism. Some genes involved in iron-metabolism are associated with genetic disorders such as Friedreich's Ataxia (Frataxin), genetic hemochromatosis (HFE), and Sex-linked anemia (Hephaestin). Hephaestin (Hp) gene was originally cloned as the gene defective in sex-linked anemia (sla) that is characterized by moderate to severe microcytic hypochronic anemia. Hp protein (mouse/rat 1157 aa, human 1158 aa, ~155kD) encodes a single-membrane spanning domain protein with extensive homology (50-60%) with copper containing serum ferrooxidase ceruloplasmin (Cp). TM domain is located very close to the C-temrinus followed by the short cytoplasmic tail. Cp is involved in release of iron from various tissues. Hp is mostly expressed throughout the small intestine and colon. Low levels are also found in some other tissues (spleen, placenta and lung). It is localized in mature villus enterocytes with little or no expression in the crypts. Hp levels are not affected by iron status.

Product Categories/Family for anti-HEPH antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 12830539
• NCBI GeneID: 9843
• UniProt Accession #: Q9BQS7; O75180; Q6UW45; Q9C058; B1AJX8; D3DVT7; Q5JZ07; Q5JZ08
• Molecular Weight: 130,449 Da
• NCBI Official Full Name: hephaestin
• NCBI Official Synonym Full Names: hephaestin
• NCBI Official Symbol: HEPH
• NCBI Official Synonym Symbols: CPL; KIAA0698
• NCBI Protein Information: hephaestin; OTTHUMP00000023443; OTTHUMP00000023444; OTTHUMP00000023445; OTTHUMP00000023446; OTTHUMP00000210958; OTTHUMP00000210959
• UniProt Protein Name: Hephaestin
• Protein Family: Hephaestin
• UniProt Gene Name: HEPH
• UniProt Synonym Gene Names: KIAA0698
• UniProt Entry Name: HEPH_HUMAN

NCBI Description

The protein encoded by this gene is similar to an iron transport protein found in mouse. The mouse protein is similar to ceruloplasmin, a serum multi-copper ferroxidase, and is thought to be a membrane-bound protein responsible for transport of dietary iron from epithelial cells of the intestinal lumen into the circulatory system. In mouse, defects in this gene can lead to severe microcytic anemia. Three transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Uniprot Description

HEPH: May function as a ferroxidase for ferrous (II) to ferric ion (III) conversion and may be involved in copper transport and homeostasis. Implicated in iron homeostasis and may mediate iron efflux associated to ferroportin 1. Belongs to the multicopper oxidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Oxidoreductase; EC 1.-.-.-

Chromosomal Location of Human Ortholog: Xq11-q12

Cellular Component: perinuclear region of cytoplasm; basolateral plasma membrane; integral to membrane; plasma membrane; intracellular

Molecular Function: ferroxidase activity; copper ion binding; ferrous iron binding

Biological Process: cellular iron ion homeostasis; copper ion transport; transmembrane transport; iron ion transport