anti-Mouse Hepcidin, pro Polyclonal Antibody | anti-HAMP antibody
Anti - Hepcidin, pro (Pro Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide)
Gene Names
HAMP; HEPC; PLTR; HFE2B; LEAP1
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Serum
Synonyms
Hepcidin; pro; Polyclonal Antibody; Anti - Hepcidin; pro (Pro Hepc; LEAP; Hepatic Bactericidal Protein; Liver Expressed Antimicrobial Peptide); anti-HAMP antibody
Host
Host: Rabbit
Source: Mouse
Source: Mouse
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
The mouse sequences is 91% conserved in rat and 63% in human pro-HEPC. This sequence is removed from the mature 20-25 secreted forms of HEPC. So antibodies will not react with the secreted forms of HEPC. Full length, human 25-aa and 20-aa HEPC and mouse 25-aa HEPC are also available for various studies.
Purity/Purification
Serum
Form/Format
Serum
Supplied as a liquid, neat serum, 40% glycerol.
Supplied as a liquid, neat serum, 40% glycerol.
Sequence Length
84
Applicable Applications for anti-HAMP antibody
ELISA, Western Blot
Application Notes
Suitable for use in Western Blotting. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for aff. pure IgG using ECL.
ELISA: ~1:100,000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Control peptides, because of the small size (2-3kD), is not recommended for Western. It should be used in ELISA or antibody blocking (use 5-10 ug control peptide per 1ug of IgG or 1ul of antiserum) experiments to demonstrate antibody specificity.
Dilution: Western Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for aff. pure IgG using ECL.
ELISA: ~1:100,000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Control peptides, because of the small size (2-3kD), is not recommended for Western. It should be used in ELISA or antibody blocking (use 5-10 ug control peptide per 1ug of IgG or 1ul of antiserum) experiments to demonstrate antibody specificity.
Immunogen
An 11-aa peptide sequence from the N-terminus (after the signal peptide) of mouse Pro-HEPC was synthesized, conjugated to KLH
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-HAMP antibody
Hepcidin (Hepc, hepatic bactericidal protein) or LEAP (liver expressed antimicrobial peptide) is small, cysteine-rich peptide, antimicrobial peptide similar to defensins and thionins. Hepcidin (unprocessed, proprotein in mouse 83 aa, rat/human 84 aa) are almost exclusively produced in liver. Human hepcidin is produced from 84-aa precursor, including a putative 24-aa signal peptide. The signal peptide is cleaved to produce pro-HEPC, which is further processed to generate the mature secreted form of hepcidin (C-terminal 20, 22 or 25-aa HEPC). In humans, 20-aa and 25-aa appears to be the major HEPC secreted peptides with antimicrobial activities. The three secreted HEPC alternatively spliced HEPC peptides differ at the N-terminus.
Product Categories/Family for anti-HAMP antibody
References
• (1). Pigeon C et al (2001) JBC 276, 7811-7819; Park CH et al (2001) JBC 276, 7806-7810, Krause A et al (2000) FEBS Lett. 480, 147-150; Fleming RE and Sly WS (2001) PNAS 99, 8160-8162; Nicolas G et al (2002) PNAS 99, 4596-4601
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
9,408 Da
NCBI Official Full Name
Hepcidin
NCBI Official Synonym Full Names
hepcidin antimicrobial peptide
NCBI Official Symbol
HAMP
NCBI Official Synonym Symbols
HEPC; PLTR; HFE2B; LEAP1
NCBI Protein Information
hepcidin; putative liver tumor regressor; liver-expressed antimicrobial peptide 1
UniProt Protein Name
Hepcidin
Protein Family
UniProt Gene Name
HAMP
UniProt Synonym Gene Names
HEPC; LEAP1
UniProt Entry Name
HEPC_HUMAN
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Product Notes
The HAMP hamp (Catalog #AAA613658) is an Antibody produced from Host: Rabbit Source: Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti - Hepcidin, pro (Pro Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Hepcidin, pro can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Suitable for use in Western Blotting. Other applications not tested. Dilution: Western Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for aff. pure IgG using ECL. ELISA: ~1:100,000 using 50-100ng control peptide/well. Optimal dilutions to be determined by the researcher. Control peptides, because of the small size (2-3kD), is not recommended for Western. It should be used in ELISA or antibody blocking (use 5-10 ug control peptide per 1ug of IgG or 1ul of antiserum) experiments to demonstrate antibody specificity. Researchers should empirically determine the suitability of the HAMP hamp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hepcidin, pro, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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