Rabbit anti-Human Hepcidin, 25aa Polyclonal Antibody
Hepcidin, 25aa (Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide)
Reactivity
Human
Applications
ELISA, Western Blot
Purity
SerumSerum
Synonyms
Hepcidin; 25aa; Polyclonal Antibody; 25aa (Hepc; LEAP; Hepatic Bactericidal Protein; Liver Expressed Antimicrobial Peptide); Anti -Hepcidin; anti-Hepcidin; 25aa antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes all 3 processed forms of human HEPC. The human sequence is conserved in all 3 human HEPC peptides (20-, 22- and 25-aa C-terminal fragments). May react with the unprocessed larger 60aa pro-HEPC if present in the samples or tissues. Species Sequence Homology
Purity/Purification
Serum
Serum
Serum
Form/Format
Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.
Applicable Applications for anti-Hepcidin, 25aa antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1:1000-1:5000 (ECL).
ELISA: 1:100,000 using 50-100ng control peptide/well.
Dilution: Western Blot: 1:1000-1:5000 (ECL).
ELISA: 1:100,000 using 50-100ng control peptide/well.
Immunogen
Synthetic peptide sequence consisting of 13aa within the C-terminus of mature 25aa Human HEPC (KLH coupled).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Hepcidin, 25aa antibody
Hepcidin (Hepc, hepatic bactericidal protein) or LEAP (liver expressed antimicrobial peptide) is small, cysteine-rich peptide, antimicrobial peptide similar to defensins and thionins. Hepcidin (unprocessed, proprotein in mouse 83aa, rat/human 84aa) are almost exclusively produced in liver. Human hepcidin is produced from 84aa precursor, including a putative 24aa signal peptide. The secreted form of hepcidin in blood and urine consists of C-terminal 20, 22 or 25aa residues. The link between hepcidins and iron metabolism is that hepcidin expression is abolished in mice exhibiting iron-overload due to the targeted disruption of USF2 (upstream stimulatory factor 2) gene resembling the situation in hfe-/- mice. The human gene is located at chromosome 19, in close proximity with Usf2 gene. Hepcidin levels are increased in iron loading and in beta-2 microglobulin knockout mice. Hepcidins are devoid of IRE. Like other antimicrobial peptides, hepcidin is upregulated by lipopolysaccharides (LPS).
Product Categories/Family for anti-Hepcidin, 25aa antibody
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Product Notes
The Hepcidin, 25aa (Catalog #AAA613809) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Hepcidin, 25aa (Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Hepcidin, 25aa can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Western Blot: 1:1000-1:5000 (ECL). ELISA: 1:100,000 using 50-100ng control peptide/well. Researchers should empirically determine the suitability of the Hepcidin, 25aa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hepcidin, 25aa, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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