Rabbit anti-Mouse Hepcidin, 13aa Polyclonal Antibody | anti-HAMP antibody
Hepcidin, CT, 13aa (Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide)
Gene Names
HAMP; hepcidin
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
SerumSerum
Synonyms
Hepcidin; 13aa; Polyclonal Antibody; CT; 13aa (Hepc; LEAP; Hepatic Bactericidal Protein; Liver Expressed Antimicrobial Peptide); Anti -Hepcidin; anti-HAMP antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse Hepcidin. May react with the unprocessed larger 60aa pro-HEPC if present in samples or tissues.
Purity/Purification
Serum
Serum
Serum
Form/Format
Supplied as a liquid in PBS, 0.05% sodium azide.
Sequence
~C-terminal of mature HEPC
Applicable Applications for anti-HAMP antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: ~1:100,000 using 50-100ng control peptide/well.
Western Blot: 1:1000-1:5000 (ECL).
Dilution: ELISA: ~1:100,000 using 50-100ng control peptide/well.
Western Blot: 1:1000-1:5000 (ECL).
Immunogen
Synthetic peptide corresponding to 13aa from mouse HEPC. Species Sequence Homology: rat, 92%; human, 69% (20-, 22- and 25aa C-terminal fragments)
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-HAMP antibody
Hepcidin (Hepc, hepatic bactericidal protein) or LEAP (liver expressed antimicrobial peptide) is small, cysteine-rich peptide, antimicrobial peptide similar to defensins and thionins. Hepcidin (unprocessed, pro-protein in mouse 83aa, rat/human 84aa) are almost exclusively produced in liver. Human hepcidin is produced from 84aa precursor, including a putative 24aa signal peptide. The secreted form of hepcidin in blood and urine is consists of C-terminal 20, 22 or 25aa residues. The link between hepcidins and iron metabolism is that hepcidin expression is abolished in mice exhibiting iron-overload due to the targeted disruption of USF2 (upstream stimulatory factor 2) gene resembling the situation in hfe-/- mice. The human gene is located at chromosome 19, in close proximity with Usf2 gene. Hepcidin levels are increased in iron loading and in beta-2 microglobulin knockout mice. Hepcidins are devoid of IRE. Like other antimicrobial peptides, hepcidin is upregulated by lipopolysaccharides (LPS).
Product Categories/Family for anti-HAMP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
hepcidin
NCBI Official Symbol
HAMP
NCBI Official Synonym Symbols
hepcidin
NCBI Protein Information
hepcidin
Protein Family
Similar Products
Product Notes
The HAMP (Catalog #AAA612401) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Hepcidin, CT, 13aa (Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Hepcidin, 13aa can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: ~1:100,000 using 50-100ng control peptide/well. Western Blot: 1:1000-1:5000 (ECL). Researchers should empirically determine the suitability of the HAMP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ~C-termina l of mature HEPC. It is sometimes possible for the material contained within the vial of "Hepcidin, 13aa, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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