Rabbit anti-Human Hepatoma Up-Regulated Protein Polyclonal Antibody | anti-HURP antibody

Hepatoma Up-Regulated Protein, NT (Disks Large-associated Protein 5, DAP-5, Disks Large-associated Protein DLG7, Discs Large Homolog 7, DLG7, HURP, DLGAP5, KIAA0008) (MaxLight 650)

Cat. Num. AAA6480065

• Gene Names: DLGAP5; DLG7; HURP
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Hepatoma Up-Regulated Protein; Polyclonal Antibody; NT (Disks Large-associated Protein 5; DAP-5; Disks Large-associated Protein DLG7; Discs Large Homolog 7; DLG7; HURP; DLGAP5; KIAA0008) (MaxLight 650); anti-HURP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DLG7.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-HURP antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human DLG7 (KLH).
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-DLG7 Antibody (N-term) in Ramos cell line lysates (35ug/lane). DLG7(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-HURP antibody

Antibodies; Proteins

References

1. de Reynies, A., J. Clin. Oncol. 27(7), 1108-1115 (2009). 2. Gudmundsson, K.O., Stem Cells 25(6), 1498-1506 (2007). 3. Laprise, P., J. Biol. Chem. 279(11), 10157-10166 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 226371667
• NCBI GeneID: 9787
• NCBI Accession #: NP_055565
• NCBI GenBank Nucleotide #: NM_014750
• UniProt Accession #: Q15398
• Molecular Weight: 95kDa
• NCBI Official Full Name: disks large-associated protein 5 isoform a
• NCBI Official Synonym Full Names: DLG associated protein 5
• NCBI Official Symbol: DLGAP5
• NCBI Official Synonym Symbols: DLG7; HURP
• NCBI Protein Information: disks large-associated protein 5
• UniProt Protein Name: Disks large-associated protein 5
• UniProt Gene Name: DLGAP5
• UniProt Synonym Gene Names: DLG7; KIAA0008; DAP-5; HURP
• UniProt Entry Name: DLGP5_HUMAN

Uniprot Description

DLG7: a cell cycle regulator that may play a role in carcinogenesis. Elevated levels of expression detected in the G2/M phase of synchronized cultures of HeLa cells. Required for chromosome congression and alignment. Stabilizes mitotic microtubules, promotes microtubule polymerization and controls spindle formation and dynamics Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Interacts with CDC2 and localizes to the spindle poles in mitotic cells. Interacts with the C-terminal proline-rich region of FBXO7. Recruited by FBXO7 to a SCF (SKP1-CUL1-F-box) protein complex in a CDC2/Cyclin B-phosphorylation dependent manner. Colocalizes with E-cadherin (CDH1) at sites of cell-cell contact in intestinal epithelial cells. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells. Two alternatively spliced isoforms have been described.

Protein type: Cell cycle regulation; Microtubule-binding

Chromosomal Location of Human Ortholog: 14q22.3

Cellular Component: cytoplasm; microtubule organizing center; spindle pole centrosome; nucleus

Molecular Function: protein binding; phosphoprotein phosphatase activity

Biological Process: cell proliferation; dephosphorylation; cell-cell signaling; positive regulation of mitotic metaphase/anaphase transition; mitotic chromosome movement towards spindle pole