Goat anti-Human Heparin Cofactor II Polyclonal Antibody | anti-SERPIND1 antibody

Heparin Cofactor II (HCII)

Cat. Num. AAA617890

• Gene Names: SERPIND1; HC2; LS2; HCF2; HCII; HLS2; D22S673
• Reactivity: Human
• Applications: ELISA
• Purity: Purified; Purified
• Synonyms: Heparin Cofactor II; Polyclonal Antibody; Heparin Cofactor II (HCII); Anti -Heparin Cofactor II (HCII); anti-SERPIND1 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Heparin Cofactor II at ~150kD.
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a liquid in 5mM HEPES, 75mM sodium chloride, pH 7.4, 50% glycerol.

• Applicable Applications for anti-SERPIND1 antibody: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA andWestern Blot.; Dilution: ELISA: 10ug/ml; Western Blot: 10ug/ml
• Immunogen: Human Heparin Cofactor II.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-SERPIND1 antibody

In the coagulation cascade, heparin cofactor II inhibits thrombin by formation of a bimolecular complex in the presence and absence of heparin. This complex is stable to denaturants and essentially irreversible. In addition to heparin, a wide variety of polyanionic compounds accelerate inhibition of thrombin by HCII. Noteworthy among these compounds is dermatan sulfate, which accelerates inhibition of thrombin by HCII, but not antithrombin III. This feature has been utilized to develop a plasma based assay specific for heparin cofactor II. Although HCII shows only ~25% sequence homology to other serpins, the reactive site peptide (COOH-terminal 36 amino acids) released on inhibition of thrombin is 53% homologous to that released from antithrombin III (AT III) by thrombin. The scissile bond in HCII is Leu-Ser which is unusual for an arginine specific protease inhibitor. In contrast to AT III, the only coagulation protease inhibited by HCII is thrombin. Other proteases inhibited by HCII include a-chymotrypsin, neutrophil cathepsin G and streptomyces griseus protease B. The major physiologic role of heparin cofactor II may remain to be discovered.

Product Categories/Family for anti-SERPIND1 antibody

Antibodies; Abs to Coagulation Factors

NCBI and Uniprot Product Information

• NCBI GI #: 183908
• NCBI GeneID: 3053
• UniProt Accession #: P05546; Q6IBZ5; B2RAI1; D3DX34; Q8IVC0
• Molecular Weight: 57,071 Da
• NCBI Official Full Name: heparin cofactor II
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade D (heparin cofactor), member 1
• NCBI Official Symbol: SERPIND1
• NCBI Official Synonym Symbols: HC2; LS2; HCF2; HCII; HLS2; D22S673
• NCBI Protein Information: heparin cofactor 2; serpin D1; leuserpin 2; OTTHUMP00000198450; OTTHUMP00000198451; protease inhibitor leuserpin-2; serine (or cysteine) proteinase inhibitor, clade D (heparin cofactor), member 1
• UniProt Protein Name: Heparin cofactor 2
• Protein Family: Heparin cofactor
• UniProt Gene Name: SERPIND1
• UniProt Synonym Gene Names: HCF2
• UniProt Entry Name: HEP2_HUMAN

NCBI Description

The product encoded by this gene is a serine proteinase inhibitor which rapidly inhibits thrombin in the presence of dermatan sulfate or heparin. The gene contains five exons and four introns. This protein shares homology with antithrombin III and other members of the alpha 1-antitrypsin superfamily. Mutations in this gene are associated with heparin cofactor II deficiency. [provided by RefSeq]

Uniprot Description

SERPIND1: Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner. Defects in SERPIND1 are the cause of thrombophilia due to heparin cofactor 2 deficiency (THPH10). A hemostatic disorder characterized by a tendency to recurrent thrombosis. Belongs to the serpin family.

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; serine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity

Biological Process: chemotaxis; blood coagulation

Disease: Heparin Cofactor Ii Deficiency