Sheep HEMOGLOBIN Polyclonal Antibody | anti-HBA2 antibody

SHEEP ANTI HUMAN HEMOGLOBIN

Cat. Num. AAA223297

• Gene Names: HBA2; HBH; HBA-T2
• Applications: ELISA, Western Blot
• Synonyms: HEMOGLOBIN; Polyclonal Antibody; SHEEP ANTI HUMAN HEMOGLOBIN; anti-HBA2 antibody

• Host: Sheep
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 5.0mg/ml (varies by lot)
• Sequence Length: 142

• Applicable Applications for anti-HBA2 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Western Blotting: Minimum Dilution: 1/100; Maximum Dilution: 1/1000
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Immunogen: Native, from erythrocytes; Buffer Solution
• Antiserum Preparation: Antisera to human hemoglobin were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography on Protein G.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Human hemoglobin detected in a red blood cell lysate using Sheep anti Human Hemoglobin (MBS223297). (Other bands detected represent different subunits of hemoglobin))

Related Product Information for anti-HBA2 antibody

Sheep anti Human Hemoglobin antibody recognizes human hemoglobin, a 66kDa metalloprotein composed of four globular subunits each of which is associated with an iron-containing heme group. The heme group is involved with oxygen transport from the lungs to the tissues and the globin molecule plays a major role in transporting carbon dioxide in the reverse direction. Hemoglobin is found in the erythrocytes of all vertebrates. There is a wide diversity of amino acid sequences and substitutions within mammalian hemoglobins, however the molecular weight is generally around 66kDa with an iron content of about 0.34%. The level of hemoglobin in the blood is used in the identification of anemia; levels in the feces are indicative of various clinical conditions. Sheep anti Human Hemoglobin antibody shows

NCBI and Uniprot Product Information

• NCBI GI #: 4504345
• NCBI GeneID: 3040
• NCBI Accession #: NP_000508.1
• NCBI GenBank Nucleotide #: NM_000517.4
• Molecular Weight: 15,258 Da
• NCBI Official Full Name: hemoglobin subunit alpha
• NCBI Official Synonym Full Names: hemoglobin, alpha 2
• NCBI Official Symbol: HBA2
• NCBI Official Synonym Symbols: HBH; HBA-T2
• NCBI Protein Information: hemoglobin subunit alpha; alpha globin; alpha-2 globin; alpha-globin; hemoglobin alpha chain
• UniProt Protein Name: Hemoglobin subunit alpha
• Protein Family: Hemoglobin
• UniProt Gene Name: HBA1
• UniProt Entry Name: HBA_HUMAN

NCBI Description

The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

HBA1: Involved in oxygen transport from the lung to the various peripheral tissues. Defects in HBA1 may be a cause of Heinz body anemias (HEIBAN). This is a form of non-spherocytic hemolytic anemia of Dacie type 1. After splenectomy, which has little benefit, basophilic inclusions called Heinz bodies are demonstrable in the erythrocytes. Before splenectomy, diffuse or punctate basophilia may be evident. Most of these cases are probably instances of hemoglobinopathy. The hemoglobin demonstrates heat lability. Heinz bodies are observed also with the Ivemark syndrome (asplenia with cardiovascular anomalies) and with glutathione peroxidase deficiency. Defects in HBA1 are the cause of alpha-thalassemia (A- THAL). The thalassemias are the most common monogenic diseases and occur mostly in Mediterranean and Southeast Asian populations. The hallmark of alpha-thalassemia is an imbalance in globin-chain production in the adult HbA molecule. The level of alpha chain production can range from none to very nearly normal levels. Deletion of both copies of each of the two alpha-globin genes causes alpha(0)-thalassemia, also known as homozygous alpha thalassemia. Due to the complete absence of alpha chains, the predominant fetal hemoglobin is a tetramer of gamma-chains (Bart hemoglobin) that has essentially no oxygen carrying capacity. This causes oxygen starvation in the fetal tissues leading to prenatal lethality or early neonatal death. The loss of three alpha genes results in high levels of a tetramer of four beta chains (hemoglobin H), causing a severe and life-threatening anemia known as hemoglobin H disease. Untreated, most patients die in childhood or early adolescence. The loss of two alpha genes results in mild alpha-thalassemia, also known as heterozygous alpha-thalassemia. Affected individuals have small red cells and a mild anemia (microcytosis). If three of the four alpha-globin genes are functional, individuals are completely asymptomatic. Some rare forms of alpha-thalassemia are due to point mutations (non- deletional alpha-thalassemia). The thalassemic phenotype is due to unstable globin alpha chains that are rapidly catabolized prior to formation of the alpha-beta heterotetramers. Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non- immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders. Defects in HBA1 are the cause of hemoglobin H disease (HBH). HBH is a form of alpha-thalassemia due to the loss of three alpha genes. This results in high levels of a tetramer of four beta chains (hemoglobin H), causing a severe and life-threatening anemia. Untreated, most patients die in childhood or early adolescence. Belongs to the globin family.

Protein type: Carrier

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: membrane; hemoglobin complex; extracellular region; cytosol

Molecular Function: haptoglobin binding; protein binding; peroxidase activity; iron ion binding; heme binding; oxygen binding; oxygen transporter activity

Biological Process: receptor-mediated endocytosis; response to hydrogen peroxide; hydrogen peroxide catabolic process; oxygen transport; bicarbonate transport; protein heterooligomerization

Disease: Hemoglobin H Disease; Heinz Body Anemias; Alpha-thalassemia