Rabbit HEC1 Polyclonal Antibody | anti-HEC1 antibody

HEC1 Polyclonal Antibody

Cat. Num. AAA3005911

• Gene Names: NDC80; HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: HEC1; Polyclonal Antibody; HEC1 Polyclonal Antibody; Kinetochore protein NDC80 homolog; Highly expressed in cancer protein; Kinetochore protein Hec1; HsHec1; Kinetochore-associated protein 2; Retinoblastoma-associated protein HEC; NDC80; KNTC2; anti-HEC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: HEC1 polyclonal antibody detects endogenous levels of HEC1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 642

• Applicable Applications for anti-HEC1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to the 388-433 of Human HEC1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of HEC1 polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysateLane2:sp2/0 cell lysateLane3:H9C2 cell lysate)

Related Product Information for anti-HEC1 antibody

Highly expressed in cancer (Hec1) is a coiled-coil-enriched protein expressed abundantly in the S and M phases of rapidly dividing cells where it localizes to the kinetochores. Hec1 is involved in spindle checkpoint signaling. Hec1 is not expressed in terminal differentiated cells. Hec1 is expressed in tissues with high mitotic rates including testis, spleen and thymus. Hec1 is also found in the late S to M phases of bladder carcinoma cells. In dividing cells, Hec1 is required for the recruitment of Mps1 kinase and MAD1/MAD2 complexes to the kinetochores. The phosphorylation of Hec1 on Serine 165 by Nek2 is essential for faithful chromosome segregation. The binding of retinoblastoma protein to Hec1 also increases the fidelity of chromosomal segregation.

NCBI and Uniprot Product Information

• NCBI GI #: 5174457
• NCBI GeneID: 10403
• NCBI Accession #: NP_006092.1
• NCBI GenBank Nucleotide #: NP_006092.1
• UniProt Accession #: O14777
• Molecular Weight: ~ 74kDa
• NCBI Official Full Name: kinetochore protein NDC80 homolog
• NCBI Official Synonym Full Names: NDC80 kinetochore complex component
• NCBI Official Symbol: NDC80
• NCBI Official Synonym Symbols: HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80
• NCBI Protein Information: kinetochore protein NDC80 homolog
• UniProt Protein Name: Kinetochore protein NDC80 homolog
• Protein Family: Transcription factor
• UniProt Gene Name: NDC80
• UniProt Synonym Gene Names: HEC; HEC1; KNTC2; HsHec1
• UniProt Entry Name: NDC80_HUMAN

NCBI Description

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]

Uniprot Description

HEC1: plays an essential role in chromosome segregation by interacting through its coiled-coil domains with several proteins that modulate the G2/M phase. Phosphorylation by Nek2 is essential for faithful chromosome segregation. Required for the recruitment of Mps1 kinase and Mad1/Mad2 complexes to kinetochores.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 18p11.32

Cellular Component: kinetochore; membrane; cytosol; nucleus; chromosome, pericentric region

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: mitosis; mitotic spindle organization and biogenesis; establishment of mitotic spindle orientation; mitotic sister chromatid segregation; attachment of spindle microtubules to kinetochore during mitosis; cell division; mitotic cell cycle; attachment of spindle microtubules to kinetochore; chromosome segregation