Rabbit anti-Human Heat Shock Protein 40 (HSP40) Polyclonal Antibody | anti-HSP40 antibody

FITC-linked Antibody to Heat Shock Protein 40 (HSP40)

Cat. Num. AAA2015709

• Gene Names: DNAJB1; Hdj1; Sis1; HSPF1; Hsp40; RSPH16B
• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Heat Shock Protein 40 (HSP40); Polyclonal Antibody; FITC-linked Antibody to Heat Shock Protein 40 (HSP40); anti-HSP40 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against HSP40 conjugated to fitc. It has been selected for its ability to recognize HSP40 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/ml (varies by lot)
• Sequence: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.; MGHHHHHHSGS-MGKDYYQTLG LARGASDEEI KRAYRRQALR YHPDKNKEPG AEEKFKEIAE AYDVLSDPRK REIFDRYGEE GLKGSGPSGG SGGGANGTSF SYTFHGDPHA MFAEFFGGRN PFDTFFGQRN GEEGMDIDDP FSGFPMGMGG FTNVNFGRSR SAQEPARKKQ DPPVTHDLRV SLEEIYSGCT KKMKISHKRL NPDGKSIRNE DKILTIEVKK GWKEGTKITF PKEGDQTSNN IPADIVFVLK DKPHNIFKRD GSDVIYPARI SLREALCGCT VNVPTLDGRT IPVVFKDVIR PGMRRKVPGE GLPLPKTPEK RGDLIIEFEV IFPERIPQTS RTVLEQVLPI
• Sequence Length: 340

• Applicable Applications for anti-HSP40 antibody: Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant HSP40 (Met1~Ile340) expressed in E.coli.
• Cross Reactivity: Human
• Unconjugated Antibody: The unconjugated antibody version of this item is also available as catalog #MBS2002675
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

((Figure. Western Blot; Sample: Recombinant protein.))

Product Categories/Family for anti-HSP40 antibody

FITC Antibody

NCBI and Uniprot Product Information

• NCBI GI #: 1816452
• NCBI GeneID: 3337
• UniProt Accession #: P25685
• Molecular Weight: 38,044 Da
• NCBI Official Full Name: heat shock protein 40
• NCBI Official Synonym Full Names: DnaJ (Hsp40) homolog, subfamily B, member 1
• NCBI Official Symbol: DNAJB1
• NCBI Official Synonym Symbols: Hdj1; Sis1; HSPF1; Hsp40; RSPH16B
• NCBI Protein Information: dnaJ homolog subfamily B member 1; hDj-1; human DnaJ protein 1; heat shock protein 40; dnaJ protein homolog 1; heat shock 40kD protein 1; radial spoke 16 homolog B; heat shock 40 kDa protein 1; DnaJ (Hsp40) homolog, subfmaily B, member 1
• UniProt Protein Name: DnaJ homolog subfamily B member 1
• UniProt Gene Name: DNAJB1
• UniProt Synonym Gene Names: DNAJ1; HDJ1; HSPF1; HSP40; Heat shock protein 40; hDj-1
• UniProt Entry Name: DNJB1_HUMAN

NCBI Description

This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

Function: Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.

Subunit structure: Interacts with DNAJC3. Interacts with SRPK1. Ref.6 Ref.7

Subcellular location: Cytoplasm. Nucleus. Nucleus › nucleolus. Note: Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock. Ref.5

Induction: By heat shock. Ref.5

Sequence similarities: Contains 1 J domain.

Sequence caution: The sequence CAA44287.1 differs from that shown. Reason: Frameshift at positions 11, 28, 81 and 136.