Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Human Heat Shock 70kDa Protein 4 (HSPA4) Polyclonal Antibody | anti-HSPA4 antibody
Polyclonal Antibody to Heat Shock 70kDa Protein 4 (HSPA4)
Gene Names
HSPA4; RY; APG-2; HSPH2; hsp70; hsp70RY; HEL-S-5a; HS24/P52
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Heat Shock 70kDa Protein 4 (HSPA4); Polyclonal Antibody; Polyclonal Antibody to Heat Shock 70kDa Protein 4 (HSPA4); anti-HSPA4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against HSPA4. It has been selected for its ability to recognize HSPA4 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- SKI RALLRLSQEC EKLKKLMSAN ASDLPLSIEC FMNDVDVSGT MNRGKFLEMC NDLLARVEPP LRSVLEQTKL KKEDIYAVEI VGGATRIPAV KEKISKFFGK ELSTTLNADE AVTRGCALQC AILSPAFKVR EFSITDVVPY PISLRWNSPA EEGSSDCEVF SKNHAAPFSK VLTFYRKEPF TLEAYYSSPQ DLPYPDPAIA
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- SKI RALLRLSQEC EKLKKLMSAN ASDLPLSIEC FMNDVDVSGT MNRGKFLEMC NDLLARVEPP LRSVLEQTKL KKEDIYAVEI VGGATRIPAV KEKISKFFGK ELSTTLNADE AVTRGCALQC AILSPAFKVR EFSITDVVPY PISLRWNSPA EEGSSDCEVF SKNHAAPFSK VLTFYRKEPF TLEAYYSSPQ DLPYPDPAIA
Sequence Length
840
Applicable Applications for anti-HSPA4 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant HSPA4 (Ser258~Glu511) expressed in E.coli.
Cross Reactivity
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2051220
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Brain tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Brain tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,689 Da
NCBI Official Full Name
heat shock 70 kDa protein 4
NCBI Official Synonym Full Names
heat shock protein family A (Hsp70) member 4
NCBI Official Symbol
HSPA4
NCBI Official Synonym Symbols
RY; APG-2; HSPH2; hsp70; hsp70RY; HEL-S-5a; HS24/P52
NCBI Protein Information
heat shock 70 kDa protein 4
UniProt Protein Name
Heat shock 70 kDa protein 4
Protein Family
UniProt Gene Name
HSPA4
UniProt Synonym Gene Names
APG2
Uniprot Description
HSP70RY: Interacts with TJP1/ZO-1. Belongs to the heat shock protein 70 family.
Protein type: Chaperone; Heat shock protein
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: cytosol; extracellular exosome
Molecular Function: ATP binding; protein binding
Biological Process: chaperone-mediated protein complex assembly; protein import into mitochondrial outer membrane; response to unfolded protein
Research Articles on HSPA4
Similar Products
Product Notes
The HSPA4 hspa4 (Catalog #AAA2017435) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Heat Shock 70kDa Protein 4 (HSPA4) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Heat Shock 70kDa Protein 4 (HSPA4) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the HSPA4 hspa4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- SKI RALLRLSQEC EKLKKLMSAN ASDLPLSIEC FMNDVDVSGT MNRGKFLEMC NDLLARVEPP LRSVLEQTKL KKEDIYAVEI VGGATRIPAV KEKISKFFGK ELSTTLNADE AVTRGCALQC AILSPAFKVR EFSITDVVPY PISLRWNSPA EEGSSDCEVF SKNHAAPFSK VLTFYRKEPF TLEAYYSSPQ DLPYPDPAIA. It is sometimes possible for the material contained within the vial of "Heat Shock 70kDa Protein 4 (HSPA4), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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