Western Blot (WB)
(Western Blot: Sample: Human Hela Cells.)
Rabbit anti-Human Heat Shock 70kDa Protein 1A Polyclonal Antibody | anti-HSPA1A antibody
Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A)
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Human Hela Cells.)
Western Blot (WB)
(Western Blot: Sample: Human Hela Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant HSPA1A, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant HSPA1A, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Esophagus Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Esophagus Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue)
Immunohistochemistry (IHC)
(FITC staining on IHC-P Simple:A549 cells))
Immunohistochemistry (IHC)
(FITC staining on IHC-P Simple:A549 cells))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Brest Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Brest Cancer Tissue)
NCBI and Uniprot Product Information
NCBI Description
This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]
Uniprot Description
HSP70: a critical chaperone protein that has a high affinity for unfolded polypeptide chains. It binds extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In cooperation with other chaperones, hsp70 stabilizes preexistent proteins against aggregation and mediates the folding of newly translated polypeptides in the cytosol as well as within organelles. Mitochondrial HSP70 is crucial to the import process: mutant forms of HSP70 fail to import precursor proteins. Is anti-apoptotic in sympathetic neurones and mediates this effect primarily by suppressing c-Jun transcriptional signalling. Interacts with tau protein and mediates proper folding of tau. Can promote the degradation of tau protein. Triptolide, a potential therapeutic agent for progression/metastasis of pancreatic cancer, causes pancreatic cancer cell death by induction of apoptosis, an effect mediated by the inhibition of HSP70.
Protein type: Chaperone; Heat shock protein; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 6p21.33
Cellular Component: aggresome; blood microparticle; centriole; centrosome; cytoplasm; cytosol; endoplasmic reticulum; extracellular exosome; extracellular region; ficolin-1-rich granule lumen; focal adhesion; inclusion body; intracellular ribonucleoprotein complex; mitochondrion; nuclear speck; nucleoplasm; nucleus; perinuclear region of cytoplasm; protein complex; ubiquitin ligase complex; vesicle
Molecular Function: ATP binding; ATPase activity; ATPase activity, coupled; cadherin binding; denatured protein binding; enzyme binding; G-protein coupled receptor binding; heat shock protein binding; histone deacetylase binding; protein binding; protein N-terminus binding; receptor binding; RNA binding; ubiquitin protein ligase binding; unfolded protein binding; viral receptor activity
Biological Process: ATP metabolic process; cellular heat acclimation; cellular response to heat; cellular response to oxidative stress; chaperone-mediated protein complex assembly; entry of virus into host cell; mRNA catabolic process; negative regulation of apoptosis; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway; negative regulation of extrinsic apoptotic signaling pathway in absence of ligand; negative regulation of inclusion body assembly; negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway; negative regulation of protein ubiquitination; negative regulation of transforming growth factor beta receptor signaling pathway; neutrophil degranulation; positive regulation of gene expression; positive regulation of interleukin-8 production; positive regulation of microtubule nucleation; positive regulation of mRNA endonucleolytic cleavage involved in unfolded protein response; positive regulation of NF-kappaB transcription factor activity; positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein refolding; protein stabilization; regulation of cell death; regulation of cellular response to heat; regulation of mitotic spindle assembly; regulation of mRNA stability; regulation of protein ubiquitination; response to unfolded protein
Research Articles on HSPA1A
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Product Notes
The HSPA1A hspa1b (Catalog #AAA2028662) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Heat Shock 70kDa Protein 1A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the HSPA1A hspa1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Heat Shock 70kDa Protein 1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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