Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Testis Tissue; Lane2: Mouse Brain Tissue; Lane3: Mouse Hela Cells.)
Rabbit anti-Mouse Heat Shock 70kDa Binding Protein 1 (HSPBP1) Polyclonal Antibody | anti-HSPBP1 antibody
Polyclonal Antibody to Heat Shock 70kDa Binding Protein 1 (HSPBP1)
Gene Names
Hspbp1; 1500019G21Rik
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Heat Shock 70kDa Binding Protein 1 (HSPBP1); Polyclonal Antibody; Polyclonal Antibody to Heat Shock 70kDa Binding Protein 1 (HSPBP1); anti-HSPBP1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against HSPBP1. It has been selected for its ability to recognize HSPBP1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF-AI SCLVREQEAG LLQFLRLDGF SVLMRAMQQQ VQKLKVKSAF LLQNLLVGHP EHKGTLCSMG MVQQLVALVR TEHSPFHEHV LGALCSLVTD FPQGVRECRE PELGLEELLR HRCQLLQQRE EYQEELEFCE KLLQTCFSSP TDDS
MGHHHHHHSGSEF-AI SCLVREQEAG LLQFLRLDGF SVLMRAMQQQ VQKLKVKSAF LLQNLLVGHP EHKGTLCSMG MVQQLVALVR TEHSPFHEHV LGALCSLVTD FPQGVRECRE PELGLEELLR HRCQLLQQRE EYQEELEFCE KLLQTCFSSP TDDS
Sequence Length
359
Applicable Applications for anti-HSPBP1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant HSPBP1 (Ala209~Ser354) expressed in E.coli.
Cross Reactivity
Human, Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2071682
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Testis Tissue; Lane2: Mouse Brain Tissue; Lane3: Mouse Hela Cells.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Testis Tissue; Lane2: Mouse Brain Tissue; Lane3: Mouse Hela Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant HSPBP1, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant HSPBP1, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,167 Da
NCBI Official Full Name
hsp70-binding protein 1
NCBI Official Synonym Full Names
HSPA (heat shock 70kDa) binding protein, cytoplasmic cochaperone 1
NCBI Official Symbol
Hspbp1
NCBI Official Synonym Symbols
1500019G21Rik
NCBI Protein Information
hsp70-binding protein 1
UniProt Protein Name
Hsp70-binding protein 1
Protein Family
UniProt Gene Name
Hspbp1
UniProt Synonym Gene Names
Hspbp; HspBP1
Uniprot Description
HSPBP1: Inhibits HSPA1A chaperone activity by changing the conformation of the ATP-binding domain of HSPA1A and interfering with ATP binding. Interferes with ubiquitination mediated by STUB1 and inhibits chaperone-assisted degradation of immature CFTR. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 7|7 A1
Molecular Function: enzyme inhibitor activity
Biological Process: positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein ubiquitination
Research Articles on HSPBP1
Similar Products
Product Notes
The HSPBP1 hspbp1 (Catalog #AAA2004064) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Heat Shock 70kDa Binding Protein 1 (HSPBP1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Heat Shock 70kDa Binding Protein 1 (HSPBP1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the HSPBP1 hspbp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-AI SCLVREQEAG LLQFLRLDGF SVLMRAMQQQ VQKLKVKSAF LLQNLLVGHP EHKGTLCSMG MVQQLVALVR TEHSPFHEHV LGALCSLVTD FPQGVRECRE PELGLEELLR HRCQLLQQRE EYQEELEFCE KLLQTCFSSP TDDS. It is sometimes possible for the material contained within the vial of "Heat Shock 70kDa Binding Protein 1 (HSPBP1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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