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Western Blot (WB) (HEK293 lysate overexpressing Human WFDC2 with C-terminal MYC tag probed with 70R-15085 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 70R-15085 (1mg/ml) in Lane B for 1 hour.Detected by chemiluminescence.)

Goat HE4 Polyclonal Antibody | anti-HE4 antibody

Goat polyclonal HE4 antibody

Applications
ELISA, Western Blot
Purity
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Synonyms
HE4; Polyclonal Antibody; Goat polyclonal HE4 antibody; HE4 antibody; anti-HE4 antibody
Ordering
Host
Goat
Clonality
Polyclonal
Purity/Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Form/Format
Supplied in Tris saline buffer, 0.02% sodium azide, pH 7.3, and 0.5% bovine serum albumin
Concentration
500 ug/ml (varies by lot)
Sequence
GAEKTGVCPELQADQ
Sequence Length
124
Applicable Applications for anti-HE4 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
ELISA: 1:16000
WB: 0.2-1 ug/ml
Immunogen
HE4 antibody was raised in goat using HE4 peptide with sequence GAEKTGVCPELQADQ as the immunogen
Cross-Reactivity
Human, Dog
Preparation and Storage
Aliquot and store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(HEK293 lysate overexpressing Human WFDC2 with C-terminal MYC tag probed with 70R-15085 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 70R-15085 (1mg/ml) in Lane B for 1 hour.Detected by chemiluminescence.)

Western Blot (WB) (HEK293 lysate overexpressing Human WFDC2 with C-terminal MYC tag probed with 70R-15085 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 70R-15085 (1mg/ml) in Lane B for 1 hour.Detected by chemiluminescence.)
Product Categories/Family for anti-HE4 antibody

NCBI and Uniprot Product Information

NCBI GI #
Molecular Weight
11,043 Da
NCBI Official Full Name
HE4 protein
UniProt Protein Name
WAP four-disulfide core domain protein 2
UniProt Gene Name
WFDC2
UniProt Synonym Gene Names
HE4; WAP5

Uniprot Description

WFDC2: a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008]

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: extracellular space

Molecular Function: aspartic-type endopeptidase inhibitor activity; endopeptidase inhibitor activity; serine-type endopeptidase inhibitor activity

Biological Process: proteolysis; spermatogenesis

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Product Notes

The HE4 wfdc2 (Catalog #AAA5306271) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HE4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: 1:16000 WB: 0.2-1 ug/ml. Researchers should empirically determine the suitability of the HE4 wfdc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GAEKTGVCPE LQADQ. It is sometimes possible for the material contained within the vial of "HE4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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