Rabbit anti-Human HDAC8 Polyclonal Antibody | anti-HDAC8 antibody

HDAC8, NT (Histone Deacetylase 8, HD8, CDA07, HDAC8, HDACL1) (Azide free) (HRP)

Cat. Num. AAA6479579

• Gene Names: HDAC8; HD8; WTS; RPD3; CDA07; CDLS5; MRXS6; HDACL1
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: HDAC8; Polyclonal Antibody; NT (Histone Deacetylase 8; HD8; CDA07; HDACL1) (Azide free) (HRP); anti-HDAC8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HDAC8.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-HDAC8 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human HDAC8 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MBS625142 in mouse 3T3 cell lysate. HDAC8 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS625142, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.)

Product Categories/Family for anti-HDAC8 antibody

Antibodies; Enzymes; Deacetylase

References

1. McDonell, N., et al., Genomics 64(3):221-229 (2000). 2. Hu, E., et al., J. Biol. Chem. 275(20):15254-15264 (2000). 3. Van den Wyngaert, I., et al., FEBS Lett. 478(1-2), 77-83 (2000). 4. Buggy, J.J., et al., Biochem. J. 350 Pt 1, 199-205 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 29839394
• NCBI GeneID: 55869
• UniProt Accession #: Q9BY41; Q86VC8; Q9NP76; Q9NYH4; A6ND12; A6ND61; A6NET3; A6NJR3; A8MQ62; B4DKN0; B4DV22
• Molecular Weight: 377
• NCBI Official Full Name: Histone deacetylase 8
• NCBI Official Synonym Full Names: histone deacetylase 8
• NCBI Official Symbol: HDAC8
• NCBI Official Synonym Symbols: HD8; WTS; RPD3; CDA07; CDLS5; MRXS6; HDACL1
• NCBI Protein Information: histone deacetylase 8; histone deacetylase-like 1
• UniProt Protein Name: Histone deacetylase 8
• UniProt Gene Name: HDAC8
• UniProt Synonym Gene Names: HDACL1; HD8
• UniProt Entry Name: HDAC8_HUMAN

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

HDAC8: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility. Belongs to the histone deacetylase family. HD type 1 subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.1.98; Hydrolase

Chromosomal Location of Human Ortholog: Xq13

Cellular Component: nucleoplasm; nuclear chromosome; histone deacetylase complex; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: NAD-dependent histone deacetylase activity (H3-K9 specific); NAD-dependent histone deacetylase activity (H3-K14 specific); metal ion binding; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription factor binding

Biological Process: chromatin assembly or disassembly; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; sister chromatid cohesion; mitotic cell cycle; chromatin modification; negative regulation of transcription from RNA polymerase II promoter

Disease: Wilson-turner X-linked Mental Retardation Syndrome