Rabbit HDAC5 Polyclonal Antibody | anti-HDAC5 antibody

HDAC5 (HD5, FLJ90614, KIAA0600, NY-CO-9, Histone Deacetylase 5)

Cat. Num. AAA620675

• Gene Names: HDAC5; HD5; NY-CO-9; FLJ90614
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Affinity Purified; Purified by Immunoaffinity chromatography.
• Synonyms: HDAC5; Polyclonal Antibody; HDAC5 (HD5; FLJ90614; KIAA0600; NY-CO-9; Histone Deacetylase 5); Anti -HDAC5 (HD5; anti-HDAC5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human histone deacetylase 5 at ~124kD. The antibody detects ~124kD. Does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 6, 7, 8, 9,10 and 11.
• Purity/Purification: Affinity Purified; Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.01% thimerosal, 30% glycerol.

• Applicable Applications for anti-HDAC5 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.; Dilution: Western Blot: 0.5-4ug/ml; Immunohistochemistry: 20ug/ml; Immunoprecipitation: 20ug/ml
• Immunogen: Synthetic peptide surrounding amino acid 575 of human HDAC-5 (Internal ID# BV-78)
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-HDAC5 antibody

Human HDAC5 is composed of 1122 amino acid residues. The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule. The N-terminal non-deacetylase domain does not show any significant homology with any published sequence. Both domains are required for HDAC5-mediated repression of gene transcription. HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions.

Product Categories/Family for anti-HDAC5 antibody

Antibodies; Abs to Histones

NCBI and Uniprot Product Information

• NCBI GI #: 62750349
• NCBI GeneID: 10014
• NCBI Accession #: NP_001015053.1
• NCBI GenBank Nucleotide #: NM_001015053.1
• UniProt Accession #: Q5W9G6; Q96K29; Q9UQL6
• Molecular Weight: 77,373 Da
• NCBI Official Full Name: histone deacetylase 5 isoform 3
• NCBI Official Synonym Full Names: histone deacetylase 5
• NCBI Official Symbol: HDAC5
• NCBI Official Synonym Symbols: HD5; NY-CO-9; FLJ90614
• NCBI Protein Information: histone deacetylase 5; antigen NY-CO-9
• UniProt Protein Name: KIAA0600 splice variant 1
• UniProt Gene Name: KIAA0600
• UniProt Entry Name: Q5W9G6_HUMAN

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

HDAC5: a transcriptional regulator of the histone deacetylase family, subfamily 2. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events. Coimmunoprecipitates only with HDAC3 family members. Interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. Two alternatively spliced isoforms have been described.

Protein type: EC 3.5.1.98; Hydrolase

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleoplasm; nuclear body; Golgi apparatus; cytoplasm; histone deacetylase complex; nucleus; cytosol

Molecular Function: NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; protein kinase C binding; NAD-dependent histone deacetylase activity (H3-K14 specific); metal ion binding; histone deacetylase binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription factor binding; transcription corepressor activity

Biological Process: establishment and/or maintenance of chromatin architecture; heart development; chromatin silencing; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; protein amino acid deacetylation; negative regulation of osteoblast differentiation; inflammatory response; response to drug; regulation of skeletal muscle fiber development; Notch signaling pathway; transcription, DNA-dependent; B cell activation; histone deacetylation; chromatin modification; response to cocaine; osteoblast development; chromatin remodeling; cellular response to insulin stimulus; regulation of protein binding; B cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of transcription, DNA-dependent; multicellular organismal response to stress