Rabbit HDAC5 Polyclonal Antibody | anti-HDAC5 antibody

HDAC5 (HD5, FLJ90614, KIAA0600, NY-CO-9, Histone Deacetylase 5) (MaxLight 650)

Cat. Num. AAA6479649

• Gene Names: HDAC5; HD5; NY-CO-9
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HDAC5; Polyclonal Antibody; HDAC5 (HD5; FLJ90614; KIAA0600; NY-CO-9; Histone Deacetylase 5) (MaxLight 650); anti-HDAC5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes HDAC5, Mr 160kD. Species Crossreactivity: Human. Weak crossreactivity with rat and mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-HDAC5 antibody: Western Blot (WB)
• Application Notes: WB: 1-4ug/ul detects HDAC5 from a HeLa nuclear extract. HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HDAC5 (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptides corresponding to amino acids 536-545 and 194-206 (CTKTGELPRQP and C-KSKEPTPGGLNHS) of human HDAC5.
• Conjugate: MaxLight650
• Positive Control: HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10ul of extract and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(WB: HeLa cell nuclear extract probed with MBS615455 HDAC5 at 1 g/ml)

Product Categories/Family for anti-HDAC5 antibody

Antibodies; Enzymes; Deacetylase

References

Grozinger, C.M., et al., PNAS, 96: 4868-4873, 1999.

NCBI and Uniprot Product Information

• NCBI GI #: 62750349
• NCBI GeneID: 10014
• NCBI Accession #: NP_001015053.1
• NCBI GenBank Nucleotide #: NM_005474
• UniProt Accession #: Q9UQL6; O60340; O60528; Q96DY4; C9JFV9
• Molecular Weight: 121,978 Da
• NCBI Official Full Name: histone deacetylase 5 isoform 3
• NCBI Official Synonym Full Names: histone deacetylase 5
• NCBI Official Symbol: HDAC5
• NCBI Official Synonym Symbols: HD5; NY-CO-9
• NCBI Protein Information: histone deacetylase 5; antigen NY-CO-9
• UniProt Protein Name: Histone deacetylase 5
• UniProt Gene Name: HDAC5
• UniProt Synonym Gene Names: KIAA0600; HD5
• UniProt Entry Name: HDAC5_HUMAN

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HDAC5: a transcriptional regulator of the histone deacetylase family, subfamily 2. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events. Coimmunoprecipitates only with HDAC3 family members. Interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. Two alternatively spliced isoforms have been described.

Protein type: EC 3.5.1.98; Hydrolase

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleoplasm; nuclear body; Golgi apparatus; histone deacetylase complex; cytoplasm; nucleus; cytosol

Molecular Function: NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; protein kinase C binding; NAD-dependent histone deacetylase activity (H3-K14 specific); histone deacetylase binding; metal ion binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription corepressor activity; transcription factor binding

Biological Process: establishment and/or maintenance of chromatin architecture; heart development; chromatin silencing; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; protein amino acid deacetylation; negative regulation of osteoblast differentiation; inflammatory response; response to drug; Notch signaling pathway; regulation of skeletal muscle fiber development; transcription, DNA-dependent; B cell activation; histone deacetylation; chromatin modification; response to cocaine; osteoblast development; chromatin remodeling; cellular response to insulin stimulus; regulation of protein binding; B cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of transcription, DNA-dependent; multicellular organismal response to stress