Rabbit HDAC3 Polyclonal Antibody | anti-HDAC3 antibody

Anti-HDAC3 antibody

Cat. Num. AAA176146

• Gene Names: HDAC3; HD3; RPD3; RPD3-2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry
• Purity: Immunogen affinity purified.
• Synonyms: HDAC3; Polyclonal Antibody; Anti-HDAC3 antibody; Histone deacetylase 3(HD3); histone deacetylase 3; HD 3 antibody; HD3 antibody; HDAC 3 antibody; HDAC3 antibody; HDAC3_HUMAN antibody; Histone deacetylase 3(HD3)(RPD3-2) antibody; histone deacetylase 3 antibody; RPD 3 antibody; RPD3 2 antibody; RPD3 antibody; RPD3-2 antibody; SMAP 45 antibody; SMAP45 antibody; anti-HDAC3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 428

• Applicable Applications for anti-HDAC3 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry ICC
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HDAC3 (411-428aa NEFYDGDHDNDKESDVEI), identical to the related rat and mouse sequences.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-HDAC3 antibody, MBS176146, IHC(P)IHC(P): Human Intestinal Cancer Tissue )

Immunohistochemistry (IHC)

(Anti-HDAC3 antibody, MBS176146, IHC(P)IHC(P): Human Lung Cancer Tissue )

Immunohistochemistry (IHC)

(Anti-HDAC3 antibody, MBS176146, IHC(P)IHC(P): Rat Intestine Tissue )

Immunohistochemistry (IHC)

(Anti-HDAC3 antibody, MBS176146, IHC(F)IHC(F): Mouse Brain Tissue )

Immunocytochemistry (ICC)

(Anti-HDAC3 antibody, MBS176146, ICCICC: A549 Cell )

Western Blot (WB)

(Anti-HDAC3 antibody, MBS176146, Western blottingLane 1: Rat Stomach Tissue LysateLane 2: Rat Testis Tissue LysateLane 3: MCF-7 Cell LysateLane 4: HELA Cell LysateLane 5: JURKAT Cell LysateLane 6: SKOV Cell Lysate )

Related Product Information for anti-HDAC3 antibody

Description: Rabbit IgG polyclonal antibody for Histone deacetylase 3(HDAC3) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, Mouse, Rat.
Background: HDAC3(HISTONE DEACETYLASE 3) is a member of the histone deacetylase/acuc/apha family of proteins that is an enzyme that in humans is encoded by the HDAC3 gene. The HDAC3 gene is mapped to 5q31.3. HDAC3 has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. The protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. And this gene is regarded as a potential tumor suppressor gene. HDAC3 has an open reading frame of 428 amino acids and shares 53% amino acid identity with HDAC1 and 52% with HDAC2. The catalytic domain of HDAC4 interacts with HDAC3 via the transcriptional corepressor NCOR2. All experimental conditions leading to the suppression of HDAC4 binding to NCOR2 and to HDAC3 resulted in loss of enzymatic activity associated with HDAC4. HDAC3 recruitment to the genome displays a circadian rhythm in mouse liver.

NCBI and Uniprot Product Information

• NCBI GI #: 3334210
• NCBI GeneID: 8841
• UniProt Accession #: O15379; O43268; Q9UEI5; Q9UEV0; D3DQE1
• Molecular Weight: 49,111 Da
• NCBI Official Full Name: Histone deacetylase 3
• NCBI Official Synonym Full Names: histone deacetylase 3
• NCBI Official Symbol: HDAC3
• NCBI Official Synonym Symbols: HD3; RPD3; RPD3-2
• NCBI Protein Information: histone deacetylase 3; SMAP45
• UniProt Protein Name: Histone deacetylase 3
• Protein Family: Histone deacetylase
• UniProt Gene Name: HDAC3
• UniProt Synonym Gene Names: HD3
• UniProt Entry Name: HDAC3_HUMAN

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HDAC3: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation. Interacts with HDAC7 and HDAC9. Forms a heterologous complex at least with YY1. Interacts with DAXX, HDAC10 and DACH1. Found in a complex with NCOR1 and NCOR2. Component of the N-Cor repressor complex, at least composed of NCOR1, NCOR2, HDAC3, TBL1X, TBL1R, CORO2A and GPS2. Interacts with BCOR, MJD2A/JHDM3A, NRIP1, PRDM6 and SRY. Interacts with BTBD14B. Interacts with GLIS2. Interacts (via the DNA-binding domain) with NR2C1; the interaction recruits phosphorylated NR2C1 to PML bodies for sumoylation. Component of the Notch corepressor complex. Interacts with CBFA2T3 and NKAP. Interacts with APEX1; the interaction is not dependent on the acetylated status of APEX1. Interacts with and deacetylates MAPK14. Interacts with ZMYND15. Widely expressed. Belongs to the histone deacetylase family. HD type 1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Deacetylase; EC 3.5.1.98; Cell cycle regulation; Apoptosis; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleoplasm; Golgi apparatus; transcriptional repressor complex; spindle microtubule; histone deacetylase complex; cytoplasm; plasma membrane; nucleus

Molecular Function: chromatin DNA binding; histone deacetylase binding; protein deacetylase activity; transcription factor binding; cyclin binding; NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; enzyme binding; NAD-dependent histone deacetylase activity (H3-K14 specific); NAD-dependent histone deacetylase activity (H4-K16 specific); chromatin binding; histone deacetylase activity; transcription corepressor activity

Biological Process: circadian rhythm; negative regulation of JNK cascade; Notch signaling pathway; establishment and/or maintenance of chromatin architecture; nerve growth factor receptor signaling pathway; transcription, DNA-dependent; regulation of mitotic cell cycle; regulation of multicellular organism growth; regulation of protein stability; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; spindle assembly; cellular lipid metabolic process; negative regulation of cell cycle; protein amino acid deacetylation; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent; positive regulation of TOR signaling pathway; negative regulation of apoptosis