Rabbit anti-Human, Mouse HDAC11 Polyclonal Antibody | anti-HDAC11 antibody

HDAC11 (Histone Deacetylase 11, HD11, HD-11, FLJ22237) (MaxLight 750)

Cat. Num. AAA6479606

• Gene Names: HDAC11; HD11
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: HDAC11; Polyclonal Antibody; HDAC11 (Histone Deacetylase 11; HD11; HD-11; FLJ22237) (MaxLight 750); anti-HDAC11 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HDAC11. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-HDAC11 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1000; WB: 1:25; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding the N-terminal region of human HDAC11 (KLH).
• Conjugate: MaxLight750
• Positive Control: Mouse Brain cell lysate
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot Analysis: Representative lot data. Mouse Brain lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-HDAC11 (1:25). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates HDAC11 (~40kD).)

Product Categories/Family for anti-HDAC11 antibody

Antibodies; Enzymes; Deacetylase

References

1. Voelter-Mahlknecht S., et al. (2005). Int J Mol Med. 16(4):589-98. 2. Bradbury CA., et al. (2005). Leukemia. 19(10):1751-9. 3. Gregoretti IV., et al. (2004). J Mol Biol. 338(1):17-31. 4. Gao, L., et al. (2002). J. Biol. Chem. 277(28):25748-25755.

NCBI and Uniprot Product Information

• NCBI GI #: 217272877
• NCBI GeneID: 79885
• NCBI Accession #: NP_079103
• NCBI GenBank Nucleotide #: NM_024827
• UniProt Accession #: Q96DB2
• Molecular Weight: 39kDa
• NCBI Official Full Name: histone deacetylase 11 isoform 1
• NCBI Official Synonym Full Names: histone deacetylase 11
• NCBI Official Symbol: HDAC11
• NCBI Official Synonym Symbols: HD11
• NCBI Protein Information: histone deacetylase 11
• UniProt Protein Name: Histone deacetylase 11
• Protein Family: Histone deacetylase
• UniProt Gene Name: HDAC11
• UniProt Synonym Gene Names: HD11

NCBI Description

This gene encodes a class IV histone deacetylase. The encoded protein is localized to the nucleus and may be involved in regulating the expression of interleukin 10. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2009]

Uniprot Description

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.