Immunohistochemistry (IHC)
(Anti-hCG receptor antibody, MBS175642, IHC(P)IHC(P): Rat Ovary Tissue )
Rabbit anti-Human, Rat hCG receptor Polyclonal Antibody | anti-LHCGR antibody
Anti-hCG receptor antibody
Concentration: 0.1-0.5ug/ml
Tested Species: Hu
Predicted Species: Rat
Immunohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1.0ug/ml
Tested Species: Rat
Predicted Species: Hu
Antigen Retrieval: By Heat
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Immunohistochemistry (IHC)
(Anti-hCG receptor antibody, MBS175642, IHC(P)IHC(P): Rat Ovary Tissue )
Immunohistochemistry (IHC)
(Anti-hCG receptor antibody, MBS175642, IHC(P)IHC(P): Rat Ovary Tissue )
Western Blot (WB)
(Anti-hCG receptor antibody, MBS175642, Western blottingAll lanes: Anti hCG receptor (MBS175642) at 0.5ug/mlWB: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 78KDObserved bind size: 78KD )
Western Blot (WB)
(Anti-hCG receptor antibody, MBS175642, Western blottingAll lanes: Anti hCG receptor (MBS175642) at 0.5ug/mlWB: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 78KDObserved bind size: 78KD )
Background: The luteinizing hormone/choriogonadotropin receptor is a member of a subfamily of G protein-coupled receptors(GPCR) characterized by the presence of a large N-terminal extracellular domain containing several leucine-rich repeats(LRR). This glycoprotein hormone receptor family has been named the LRR-containing GPCR(LGR) family. The LHCGR gene contains 11 exons and spans approximately 80 kb and it is mapped to chromosome 2p21. In the ovary, theca, stromal, late-stage(luteinizing) granulosa, and luteal cells contain LHCGR. In the testes, Leydig cells contain LHCRG.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the receptor for both luteinizing hormone and choriogonadotropin. This receptor belongs to the G-protein coupled receptor 1 family, and its activity is mediated by G proteins which activate adenylate cyclase. Mutations in this gene result in disorders of male secondary sexual character development, including familial male precocious puberty, also known as testotoxicosis, hypogonadotropic hypogonadism, Leydig cell adenoma with precocious puberty, and male pseudohermaphtoditism with Leydig cell hypoplasia. [provided by RefSeq, Jul 2008]
Uniprot Description
LHR: Receptor for lutropin-choriogonadotropic hormone. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Defects in LHCGR are a cause of familial male precocious puberty (FMPP); also known as testotoxicosis. In FMPP the receptor is constitutively activated. Defects in LHCGR are the cause of luteinizing hormone resistance (LHR); also known as Leydig cell hypoplasia in males. LHR is an autosomal recessive disorder characterized by unresponsiveness to luteinizing hormone, defective sexual development in males, and defective follicular development and ovulation, amenorrhea and infertility in females. Two forms of the disorder have been defined in males. Type 1 is a severe form characterized by complete 46,XY male pseudohermaphroditism, low testosterone and high luteinizing hormone levels, total lack of responsiveness to luteinizing and chorionic gonadotropin hormones, lack of breast development, and absent development of secondary male sex characteristics. Type 2, a milder form, displays a broader range of phenotypic expression ranging from micropenis to severe hypospadias. Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 2p21
Cellular Component: integral to plasma membrane; plasma membrane; endosome
Molecular Function: lutropin-choriogonadotropic hormone receptor activity
Biological Process: G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to cyclic nucleotide second messenger; male gonad development; adenylate cyclase activation; male genitalia development; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of inositol trisphosphate biosynthetic process; luteinizing hormone signaling pathway; cognition
Disease: Precocious Puberty, Male-limited; Leydig Cell Hypoplasia, Type I
Research Articles on LHCGR
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Product Notes
The LHCGR lhcgr (Catalog #AAA175642) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-hCG receptor antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's hCG receptor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot: Concentration: 0.1-0.5ug/ml Tested Species: Hu Predicted Species: Rat Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1.0ug/ml Tested Species: Rat Predicted Species: Hu Antigen Retrieval: By Heat Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the LHCGR lhcgr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "hCG receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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