Flow Cytometry (FC/FACS)
(Flow cytometric analysis of U-87 MG cells using HBG2 Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). MBS9202729 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
Rabbit anti-Human, mouse HBG2 Polyclonal Antibody | anti-HBG2 antibody
HBG2 Antibody (C-term)
IHC-P~~1:25
WB~~1:1000
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of U-87 MG cells using HBG2 Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). MBS9202729 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of U-87 MG cells using HBG2 Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). MBS9202729 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H. liver section using HBG2 Antibody (C-term). MBS9202729 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H. liver section using HBG2 Antibody (C-term). MBS9202729 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded M. liver section using HBG2 Antibody (C-term). MBS9202729 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded M. liver section using HBG2 Antibody (C-term). MBS9202729 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Western Blot (WB)
(Western blot analysis of lysates from human brain, mouse brain and eyeball tissue lysate (from left to right), using HBG2 Antibody (C-term). MBS9202729 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from human brain, mouse brain and eyeball tissue lysate (from left to right), using HBG2 Antibody (C-term). MBS9202729 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Cavallesco C.,et al.Gene 12:215-221(1980).
Kutlar F.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Schroeder W.A.,et al.Biochemistry 2:992-1008(1963).
NCBI and Uniprot Product Information
NCBI Description
The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'- epsilon -- gamma-G -- gamma-A -- delta -- beta--3'. [provided by RefSeq, Jul 2008]
Uniprot Description
HBG2: Gamma chains make up the fetal hemoglobin F, in combination with alpha chains. Defects in HBG2 are the cause of cyanosis transient neonatal (TNCY). TNCY is a disorder characterized by cyanosis in the fetus and neonate, due to a defect in the fetal hemoglobin chain which has reduced affinity for oxygen. Some patients develop anemia resulting from increased destruction of red cells containing abnormal or unstable hemoglobin. The cyanosis resolves spontaneously by 5 to 6 months of age or earlier, as the adult beta-globin chain is produced and replaces the fetal gamma- globin chain. Belongs to the globin family.
Protein type: Carrier
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: hemoglobin complex; cytosol
Molecular Function: iron ion binding; heme binding; oxygen binding; oxygen transporter activity
Biological Process: oxygen transport; blood coagulation
Disease: Cyanosis, Transient Neonatal; Fetal Hemoglobin Quantitative Trait Locus 1
Research Articles on HBG2
Similar Products
Product Notes
The HBG2 hbg2 (Catalog #AAA9202729) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HBG2 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HBG2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry-Paraffin (IHC-P). FC~~1:25 IHC-P~~1:25 WB~~1:1000. Researchers should empirically determine the suitability of the HBG2 hbg2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HBG2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
