Rabbit anti-Human HAT1 Polyclonal Antibody | anti-HAT1 antibody

HAT1, NT (Histone Acetyltransferase Type B Catalytic Subunit) (Azide free) (HRP)

Cat. Num. AAA6479511

• Gene Names: HAT1; KAT1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: HAT1; Polyclonal Antibody; NT (Histone Acetyltransferase Type B Catalytic Subunit) (Azide free) (HRP); anti-HAT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HAT1.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-HAT1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human HAT1 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of KAT1 (HAT1) Antibody (N-term) in HL60 cell line lysates (35ug/lane). HAT1 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-HAT1 antibody

Antibodies; Enzymes; Acetyltransferase

References

1. Gronroos, E., et al., Mol. Cell 10(3):483-493 (2002). 2. Makowski, A.M., et al., J. Biol. Chem. 276(47):43499-43502 (2001). 3. Cheung, P., et al., Mol. Cell 5(6):905-915 (2000). 4. Verreault, A., et al., Curr. Biol. 8(2):96-108 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 4504341
• NCBI GeneID: 8520
• NCBI Accession #: NP_003633
• NCBI GenBank Nucleotide #: NM_003642.3
• UniProt Accession #: O14929; Q49A44; Q53QF0; Q53SU4; Q6P594; Q8WWB9
• Molecular Weight: 40.1 kDa (343aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: histone acetyltransferase type B catalytic subunit
• NCBI Official Synonym Full Names: histone acetyltransferase 1
• NCBI Official Symbol: HAT1
• NCBI Official Synonym Symbols: KAT1
• NCBI Protein Information: histone acetyltransferase type B catalytic subunit
• UniProt Protein Name: Histone acetyltransferase type B catalytic subunit
• Protein Family: HAT1-interacting factor
• UniProt Gene Name: HAT1
• UniProt Synonym Gene Names: KAT1
• UniProt Entry Name: HAT1_HUMAN

NCBI Description

The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jun 2009]

Uniprot Description

HAT1: Acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage. Belongs to the HAT1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.1.48; Acetyltransferase

Chromosomal Location of Human Ortholog: 2q31.2-q33.1

Cellular Component: nucleoplasm; nuclear matrix; protein complex; intracellular membrane-bound organelle; cytoplasm; nuclear chromatin; nucleus

Molecular Function: H4 histone acetyltransferase activity; protein binding; histone acetyltransferase activity

Biological Process: establishment and/or maintenance of chromatin architecture; DNA replication-independent nucleosome assembly; internal protein amino acid acetylation; DNA replication-dependent nucleosome assembly; DNA packaging; response to nutrient; chromatin silencing at telomere