Western Blot (WB)
(Gel: 8%SDS-PAGE Lysate: 40 μ g Lane 1-2: Human fetal liver and mouse liver tissue Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 seconds)
Rabbit anti-Human, Mouse HAO1 Polyclonal Antibody | anti-HAO1 antibody
HAO1 Antibody
Gene Names
HAO1; GOX; GOX1; HAOX1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purification
Synonyms
HAO1; Polyclonal Antibody; HAO1 Antibody; GOX; GOX1; HAOX1; anti-HAO1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total HAO1 protein.
Purity/Purification
Antigen Affinity Purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
370
Applicable Applications for anti-HAO1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:25-1:100
Immunohistochemistry: 1:25-1:100
Immunogen
Fusion protein of human HAO1
Immunogen Type
Protein
Preparation and Storage
Store at -20 degree C
Western Blot (WB)
(Gel: 8%SDS-PAGE Lysate: 40 μ g Lane 1-2: Human fetal liver and mouse liver tissue Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 seconds)
Western Blot (WB)
(Gel: 8%SDS-PAGE Lysate: 40 μ g Lane 1-2: Human fetal liver and mouse liver tissue Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 seconds)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using at dilution 1/20. (Original magnification: ¡200))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using at dilution 1/20. (Original magnification: ¡200))
Related Product Information for anti-HAO1 antibody
This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site.
Product Categories/Family for anti-HAO1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,924 Da
NCBI Official Full Name
hydroxyacid oxidase 1
NCBI Official Synonym Full Names
hydroxyacid oxidase 1
NCBI Official Symbol
HAO1
NCBI Official Synonym Symbols
GOX; GOX1; HAOX1
NCBI Protein Information
hydroxyacid oxidase 1
UniProt Protein Name
Hydroxyacid oxidase 1
Protein Family
UniProt Gene Name
HAO1
UniProt Synonym Gene Names
GOX1; HAOX1; HAOX1; GOX
UniProt Entry Name
HAOX1_HUMAN
Uniprot Description
HAO1: Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2- hydroxy octanoate. Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.
Protein type: Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 1.1.3.15; Oxidoreductase
Chromosomal Location of Human Ortholog: 20p12
Cellular Component: peroxisomal matrix; peroxisome
Molecular Function: (S)-2-hydroxy-acid oxidase activity; FMN binding; glycolate oxidase activity; glyoxylate oxidase activity; receptor binding
Biological Process: fatty acid alpha-oxidation; glycolate catabolic process; glyoxylate metabolic process
Similar Products
Product Notes
The HAO1 hao1 (Catalog #AAA9415247) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HAO1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HAO1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1:500-1:2000 Immunohistochemistry: 1:25-1:100. Researchers should empirically determine the suitability of the HAO1 hao1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HAO1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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