Rabbit anti-Human, Rat HAND1 Polyclonal Antibody | anti-HAND1 antibody

HAND1 Polyclonal Antibody

Cat. Num. AAA3007178

• Gene Names: HAND1; Hxt; eHand; Thing1; bHLHa27
• Reactivity: Human, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: HAND1; Polyclonal Antibody; HAND1 Polyclonal Antibody; Heart- and neural crest derivatives-expressed protein 1; Class A basic helix-loop-helix protein 27; bHLHa27; Extraembryonic tissues; heart; autonomic nervous system and neural crest derivatives-expressed protein 1; eHAND; BHLHA27; EHAND; anti-HAND1 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: HAND1 polyclonal antibody detects endogenous levels of HAND1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 215

• Applicable Applications for anti-HAND1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human HAND1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of HAND1 polyclonal antibody at 1:500Line1:Hela whole cell lysateLine2:H9C2 whole cell lysateLine3:)

Related Product Information for anti-HAND1 antibody

dHAND (for deciduum, heart, autonomic nervous system and neural crest derivatives; also designated HAND2) and eHAND (also designated HAND1, HXT or Thing1) are members of a subclass of basic-helix-loop-helix transcription factors that are involved in cardiac development. dHAND and eHAND are expressed in the heart after cardiac looping, and they participate in left-right cardiac asymmetry. dHAND is expressed predominantly on the right side of the looped heart tube and in the pulmonary ventricle, where it activates transcription of various genes, including Ufd1 (for ubiquitin fusion degradation) and Cdc45. In addition, dHAND is expressed in sympathetic neurons and chromafin cells throughout embryonic and fetal development and mediates neural crest development. eHAND expression is primarily observed on the left side and in the systemic ventricle, suggesting that these proteins are involved in the development of segments of the heart tube, which give rise to specific heart chambers during cardiogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 4758506
• NCBI GeneID: 9421
• NCBI Accession #: NP_004812.1
• NCBI GenBank Nucleotide #: NP_004812.1
• UniProt Accession #: O96004
• Molecular Weight: ~ 24kDa
• NCBI Official Full Name: heart- and neural crest derivatives-expressed protein 1
• NCBI Official Synonym Full Names: heart and neural crest derivatives expressed 1
• NCBI Official Symbol: HAND1
• NCBI Official Synonym Symbols: Hxt; eHand; Thing1; bHLHa27
• NCBI Protein Information: heart- and neural crest derivatives-expressed protein 1
• UniProt Protein Name: Heart- and neural crest derivatives-expressed protein 1
• Protein Family: Heart- and neural crest derivatives-expressed protein
• UniProt Gene Name: HAND1
• UniProt Synonym Gene Names: BHLHA27; EHAND; bHLHa27; eHAND
• UniProt Entry Name: HAND1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

HAND1: Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'- NRTCTG-3' (non-canonical E-box).

Protein type: Transcription factor; Nucleolus; Cell development/differentiation; DNA-binding

Chromosomal Location of Human Ortholog: 5q33

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; enzyme binding; protein heterodimerization activity; transcription coactivator activity; bHLH transcription factor binding; transcription factor binding; transcription corepressor activity

Biological Process: transcription from RNA polymerase II promoter; blastocyst development; negative regulation of transcription factor activity; heart development; negative regulation of transcription from RNA polymerase II promoter; odontogenesis of dentine-containing teeth; trophectodermal cell differentiation; mesoderm formation; ventricular cardiac muscle morphogenesis; embryonic heart tube development; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; heart looping; negative regulation of transcription, DNA-dependent