Rabbit anti-Human HAMP Polyclonal Antibody | anti-HAMP antibody

HAMP, ID (HAMP, HEPC, LEAP1, Hepcidin, Liver-expressed antimicrobial peptide 1, Putative liver tumor regressor, Hepcidin-25, Hepcidin-20) (AP)

Cat. Num. AAA6305361

• Gene Names: HAMP; HEPC; PLTR; HFE2B; LEAP1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HAMP; Polyclonal Antibody; ID (HAMP; HEPC; LEAP1; Hepcidin; Liver-expressed antimicrobial peptide 1; Putative liver tumor regressor; Hepcidin-25; Hepcidin-20) (AP); Hepcidin-20; anti-HAMP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-HAMP antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: HAMP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24~54 amino acids from the Central region of human HAMP.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of HAMP Antibody (Center) in MDA-MB435 cell line lysates (35ug/lane). HAMP (arrow) was detected using the purified Pab.)

Related Product Information for anti-HAMP antibody

HAMP is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity.

Product Categories/Family for anti-HAMP antibody

Antibodies; Cancer Markers

References

Matsumoto, M., et al. Circ. J. 74(2):301-306(2010). del Giudice, E.M., et al. J. Clin. Endocrinol. Metab. 94(12):5102-5107(2009). Kwapisz, J., et al. J Zhejiang Univ Sci B 10(11):791-795(2009). Barton, J.C., et al. Am. J. Hematol. 84(11):710-714(2009). Nemeth, E., et al. Acta Haematol. 122 (2-3), 78-86 (2009). Hunter, H.N., et al. J. Biol. Chem. 277(40):37597-37603(2002). Kluver, E., et al. J. Pept. Res. 59(6):241-248(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 10863973
• NCBI GeneID: 57817
• NCBI Accession #: NP_066998.1
• NCBI GenBank Nucleotide #: NM_021175.2
• UniProt Accession #: P81172; Q1HE14; Q9BY68
• Molecular Weight: 9,408 Da
• NCBI Official Full Name: hepcidin preproprotein
• NCBI Official Synonym Full Names: hepcidin antimicrobial peptide
• NCBI Official Symbol: HAMP
• NCBI Official Synonym Symbols: HEPC; PLTR; HFE2B; LEAP1
• NCBI Protein Information: hepcidin; putative liver tumor regressor; liver-expressed antimicrobial peptide 1
• UniProt Protein Name: Hepcidin
• Protein Family: Hepcidin
• UniProt Gene Name: HAMP
• UniProt Synonym Gene Names: HEPC; LEAP1; LEAP-1; PLTR; Hepc25; Hepc20
• UniProt Entry Name: HEPC_HUMAN

NCBI Description

The product encoded by this gene is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity. Mutations in this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that results in cardiomyopathy, cirrhosis, and endocrine failure. [provided by RefSeq, Jul 2008]

Uniprot Description

HAMP: Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages. Defects in HAMP are the cause of hemochromatosis type 2B (HFE2B); also known as juvenile hemochromatosis (JH). HFE2B is a disorder of iron metabolism with excess deposition of iron in the tissues, bronze skin pigmentation, hepatic cirrhosis, arthropathy and diabetes. The most common symptoms of hemochromatosis type 2 at presentation are hypogonadism and cardiomyopathy. Belongs to the hepcidin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: apical cortex; extracellular region

Molecular Function: hormone activity

Biological Process: killing of cells of another organism; cellular iron ion homeostasis; defense response to bacterium; immune response; defense response to fungus

Disease: Hemochromatosis, Type 2b