Western Blot (WB)
(Western Blot analysis in Ramos cell line lysates (35ug/lane) usingMBS642483. This demonstrates that MBS642483 detected the GUCY2D protein (arrow).)
Rabbit anti-Human, Rat GUCY2D Polyclonal Antibody | anti-GUCY2D antibody
GUCY2D, ID (GUCY2D, CORD6, GUC1A4, GUC2D, RETGC, RETGC1, Retinal guanylyl cyclase 1, Guanylate cyclase 2D, retinal, Rod outer segment membrane guanylate cyclase) (MaxLight 405)
Western Blot (WB)
(Western Blot analysis in Ramos cell line lysates (35ug/lane) usingMBS642483. This demonstrates that MBS642483 detected the GUCY2D protein (arrow).)
Western Blot (WB)
(Western Blot analysis in Ramos cell line lysates (35ug/lane) usingMBS642483. This demonstrates that MBS642483 detected the GUCY2D protein (arrow).)
Western Blot (WB)
(Western Blot analysis in rat eyeball lysate (20ug/lane) using MBS642483 (1:2000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 120kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western Blot analysis in rat eyeball lysate (20ug/lane) using MBS642483 (1:2000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 120kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue using MBS642483 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS642483 for immunohistochemistry.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue using MBS642483 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS642483 for immunohistochemistry.)
Testing Data
(Overlay histogram showing Hela cells stained with MBS642483 (green line). The cells were fixed with 2% paraformaldehyde (10mins). The cells were then incubated in 2% BSA to block non-specific protein-protein interactions followed by MBS642483 (1:25) for 60mins at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1:200 for 40mins at 37 degree C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10e6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Testing Data
(Overlay histogram showing Hela cells stained with MBS642483 (green line). The cells were fixed with 2% paraformaldehyde (10mins). The cells were then incubated in 2% BSA to block non-specific protein-protein interactions followed by MBS642483 (1:25) for 60mins at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1:200 for 40mins at 37 degree C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10e6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
NCBI and Uniprot Product Information
Uniprot Description
GUCY2D: Probably plays a specific functional role in the rods and/or cones of photoreceptors. It may be the enzyme involved in the resynthesis of cGMP required for recovery of the dark state after phototransduction. Defects in GUCY2D are the cause of Leber congenital amaurosis type 1 (LCA1). LCA designates a clinically and genetically heterogeneous group of childhood retinal degenerations, generally inherited in an autosomal recessive manner. Affected infants have little or no retinal photoreceptor function as tested by electroretinography. LCA represents the most common genetic cause of congenital visual impairment in infants and children. Defects in GUCY2D are the cause of cone-rod dystrophy type 6 (CORD6). CORDs are inherited retinal dystrophies belonging to the group of pigmentary retinopathies. CORDs are characterized by retinal pigment deposits visible on fundus examination, predominantly in the macular region, and initial loss of cone photoreceptors followed by rod degeneration. This leads to decreased visual acuity and sensitivity in the central visual field, followed by loss of peripheral vision. Severe loss of vision occurs earlier than in retinitis pigmentosa. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
Protein type: Nucleotide Metabolism - purine; Guanylyl cyclase; Membrane protein, integral; Protein kinase, RGC; Protein kinase, dual-specificity (receptor); EC 4.6.1.2; Lyase; Receptor, misc.; Kinase, protein; RGC group; RGC family
Chromosomal Location of Human Ortholog: 17p13.1
Cellular Component: nuclear outer membrane; integral to plasma membrane
Molecular Function: guanylate cyclase activity; GTP binding; receptor activity; ATP binding; protein kinase activity
Biological Process: rhodopsin mediated signaling; cGMP biosynthetic process; phototransduction, visible light; regulation of rhodopsin mediated signaling; visual perception; receptor guanylyl cyclase signaling pathway; protein amino acid phosphorylation
Disease: Leber Congenital Amaurosis 1; Cone-rod Dystrophy 6
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Product Notes
The GUCY2D gucy2d (Catalog #AAA6305113) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GUCY2D, ID (GUCY2D, CORD6, GUC1A4, GUC2D, RETGC, RETGC1, Retinal guanylyl cyclase 1, Guanylate cyclase 2D, retinal, Rod outer segment membrane guanylate cyclase) (MaxLight 405) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GUCY2D can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GUCY2D gucy2d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GUCY2D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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