Western Blot (WB)
(Figure: Western Blot of whole cell lysate (left) and Immunoprecipitation (right) of the human sGCb1 subunit with MBS610963.)
Rabbit anti-Human Guanylyl Cyclase, beta1, Soluble Polyclonal Antibody | anti-GUCY1B3 antibody
Guanylyl Cyclase, beta1, Soluble (sGCb1)
Gene Names
GUCY1B3; GUCY1B1
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
SerumSerum
Synonyms
Guanylyl Cyclase; beta1; Soluble; Polyclonal Antibody; Soluble (sGCb1); Anti -Guanylyl Cyclase; anti-GUCY1B3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human sCG beta1.
Purity/Purification
Serum
Serum
Serum
Form/Format
Supplied as a liquid, 40% glycerol. No preservatives added.
Applicable Applications for anti-GUCY1B3 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in Western Blot and Immunoprecipitation.
Dilution: Immunoprecipitation: 1:100
Western Blot: 1:500-1:2500.
Not recommended for immunohistochemistry or functional applications (e.g. inhibition of soluble guanylyl cyclase). Optimal dilutions to be determined by the researcher.
Dilution: Immunoprecipitation: 1:100
Western Blot: 1:500-1:2500.
Not recommended for immunohistochemistry or functional applications (e.g. inhibition of soluble guanylyl cyclase). Optimal dilutions to be determined by the researcher.
Immunogen
Catalytic domain (aa 404-619) of human sCG beta1 (soluble guanylyl cyclase beta1 subunit).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Figure: Western Blot of whole cell lysate (left) and Immunoprecipitation (right) of the human sGCb1 subunit with MBS610963.)
Western Blot (WB)
(Figure: Western Blot of whole cell lysate (left) and Immunoprecipitation (right) of the human sGCb1 subunit with MBS610963.)
Product Categories/Family for anti-GUCY1B3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
70,502 Da
NCBI Official Full Name
Guanylate cyclase soluble subunit beta-1
NCBI Official Symbol
GUCY1B3
NCBI Official Synonym Symbols
GUCY1B1
NCBI Protein Information
guanylate cyclase soluble subunit beta-1; GCS-beta-1; GCS-beta-3; NO-sensitive GC beta 1 subunit; soluble guanylate cyclase small subunit; guanylate cyclase soluble subunit beta-3; nitric oxide-sensitive guanylyl cyclase beta 1 subunit
UniProt Protein Name
Guanylate cyclase soluble subunit beta-1
Protein Family
UniProt Gene Name
GUCY1B1
UniProt Synonym Gene Names
GUC1B3; GUCY1B3
UniProt Entry Name
GCYB1_BOVIN
Uniprot Description
Catalytic activity: GTP = 3',5'-cyclic GMP + diphosphate.
Cofactor: Binds 1 or 2 heme groups per heterodimer.
Enzyme regulation: Activated by nitric oxide in the presence of magnesium or manganese ions, binding of NO to the heme iron increases catalytic activity up to 400 folds.
Subunit structure: Heterodimer of an alpha and a beta chain.
Subcellular location: Cytoplasm.
Tissue specificity: Lung and brain.
Miscellaneous: There are two types of guanylate cyclases: soluble forms and membrane-associated receptor forms.
Sequence similarities: Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.Contains 1 guanylate cyclase domain.
Similar Products
Product Notes
The GUCY1B3 gucy1b1 (Catalog #AAA610963) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Guanylyl Cyclase, beta1, Soluble (sGCb1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Guanylyl Cyclase, beta1, Soluble can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Suitable for use in Western Blot and Immunoprecipitation. Dilution: Immunoprecipitation: 1:100 Western Blot: 1:500-1:2500. Not recommended for immunohistochemistry or functional applications (e.g. inhibition of soluble guanylyl cyclase). Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the GUCY1B3 gucy1b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Guanylyl Cyclase, beta1, Soluble, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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