Rabbit anti-Human, Mouse Guanylate Cyclase alpha-2, Soluble Polyclonal Antibody | anti-GC-SA2 antibody

Guanylate Cyclase alpha-2, Soluble, NT (Guanylate Cyclase Soluble Subunit alpha-2, Guanylate Cyclase 1 Soluble alpha 2, GCS-alpha-2, GC-SA2, GUC1A2, GUCSA2, GUCY1A2) (Biotin)

Cat. Num. AAA6479311

• Gene Names: GUCY1A2; GC-SA2; GUC1A2
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Guanylate Cyclase alpha-2; Soluble; Polyclonal Antibody; NT (Guanylate Cyclase Soluble Subunit alpha-2; Guanylate Cyclase 1 Soluble alpha 2; GCS-alpha-2; GC-SA2; GUC1A2; GUCSA2; GUCY1A2) (Biotin); EC=4.6.1.2; anti-GC-SA2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GUCY1A2. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-GC-SA2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human GUCY1A2 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(Western blot analysis of anti-GUCY1A2 Antibody (N-term) in mouse cerebellum tissue lysates (35ug/lane). GUCY1A2 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-GC-SA2 antibody

Antibodies; Enzymes; Cyclase

References

1. Baba, H., et al., Neuroreport 15(4):677-680 (2004). 2. Bellamy, T.C., et al., Proc. Natl. Acad. Sci. USA 99(1):507-510 (2002). 3. Russwurm, M., et al., J. Biol. Chem. 276(48):44647-44652 (2001). 4. Bamberger, A.M., et al., J. Clin. Endocrinol. Metab. 86(2):909-912 (2001). 5. Budworth, J., et al., Biochem. Biophys. Res. Commun. 263(3):696-701 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 4504211
• NCBI GeneID: 2977
• NCBI Accession #: NP_000846
• NCBI GenBank Nucleotide #: NM_000855.2
• UniProt Accession #: P33402; A1L4C4; B7ZLT5
• Molecular Weight: 83,986 Da
• NCBI Official Full Name: guanylate cyclase soluble subunit alpha-2 isoform 2
• NCBI Official Synonym Full Names: guanylate cyclase 1 soluble subunit alpha 2
• NCBI Official Symbol: GUCY1A2
• NCBI Official Synonym Symbols: GC-SA2; GUC1A2
• NCBI Protein Information: guanylate cyclase soluble subunit alpha-2
• UniProt Protein Name: Guanylate cyclase soluble subunit alpha-2
• UniProt Gene Name: GUCY1A2
• UniProt Synonym Gene Names: GUC1A2; GUCSA2
• UniProt Entry Name: GCYA2_HUMAN

NCBI Description

Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

GUCY1A2: Has guanylyl cyclase on binding to the beta-1 subunit. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lyase; Nucleotide Metabolism - purine; EC 4.6.1.2; Guanylyl cyclase

Chromosomal Location of Human Ortholog: 11q21-q22

Cellular Component: cytoplasm

Molecular Function: guanylate cyclase activity; GTP binding; heme binding

Biological Process: cGMP biosynthetic process; positive regulation of cGMP biosynthetic process; blood coagulation; signal transduction