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Western Blot (WB)
(Western blot analysis of extracts from HepG2, using GTF3C2 Antibody. Lane 1 was treated with the antigen-specific peptide.)
Rabbit anti-Human GTF3C2 Polyclonal Antibody | anti-GTF3C2 antibody
GTF3C2 Antibody
Gene Names
GTF3C2; TFIIIC110; TFIIIC-BETA
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
GTF3C2; Polyclonal Antibody; GTF3C2 Antibody; General transcription factor 3C polypeptide 2; General transcription factor IIIC polypeptide 2 beta 110kDa; Gtf3c2; KIAA0011; TF3C beta; TF3C-beta; TF3C2_HUMAN; TFIIIC 110 kDa subunit; TFIIIC BETA; TFIIIC110; Transcription factor IIIC 110 kDa subunit; Transcription factor IIIC subunit beta; anti-GTF3C2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
GTF3C2 antibody detects endogenous levels of total GTF3C2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
911
Applicable Applications for anti-GTF3C2 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Nucleus.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (82%), Bovine (92%), Horse (100%), Sheep (92%), Rabbit (92%), Dog (92%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from HepG2, using GTF3C2 Antibody. Lane 1 was treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HepG2, using GTF3C2 Antibody. Lane 1 was treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts from 293, using GTF3C2 Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from 293, using GTF3C2 Antibody.)
Immunofluorescence (IF)
(MBS9604833 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescence (IF)
(MBS9604833 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells using TF3C2 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells using TF3C2 antibody.)
Related Product Information for anti-GTF3C2 antibody
Function: Required for RNA polymerase III-mediated transcription. Component of TFIIIC that initiates transcription complex assembly on tRNA and is required for transcription of 5S rRNA and other stable nuclear and cytoplasmic RNAs. May play a direct role in stabilizing interactions of TFIIIC2 with TFIIIC1.
Subunit Structure: Part of the TFIIIC subcomplex TFIIIC2, consisting of six subunits, GTF3C1, GTF3C2, GTF3C3, GTF3C4, GTF3C5 and GTF3C6.
Subunit Structure: Part of the TFIIIC subcomplex TFIIIC2, consisting of six subunits, GTF3C1, GTF3C2, GTF3C3, GTF3C4, GTF3C5 and GTF3C6.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 100 kD
Predicted: 101 kDa
Predicted: 101 kDa
NCBI Official Full Name
general transcription factor 3C polypeptide 2 isoform b
NCBI Official Synonym Full Names
general transcription factor IIIC subunit 2
NCBI Official Symbol
GTF3C2
NCBI Official Synonym Symbols
TFIIIC110; TFIIIC-BETA
NCBI Protein Information
general transcription factor 3C polypeptide 2
UniProt Protein Name
General transcription factor 3C polypeptide 2
Protein Family
UniProt Gene Name
GTF3C2
UniProt Synonym Gene Names
KIAA0011; TFIIIC 110 kDa subunit; TFIIIC110
Uniprot Description
Required for RNA polymerase III-mediated transcription. Component of TFIIIC that initiates transcription complex assembly on tRNA and is required for transcription of 5S rRNA and other stable nuclear and cytoplasmic RNAs. May play a direct role in stabilizing interactions of TFIIIC2 with TFIIIC1.
Research Articles on GTF3C2
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Product Notes
The GTF3C2 gtf3c2 (Catalog #AAA9604833) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GTF3C2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GTF3C2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the GTF3C2 gtf3c2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GTF3C2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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