Rabbit GTF2H5 Polyclonal Antibody | anti-GTF2H5 antibody

GTF2H5 Antibody

Cat. Num. AAA9611804

• Gene Names: GTF2H5; TTD; TFB5; TTD3; TTDA; TFIIH; TTD-A; TGF2H5; C6orf175; bA120J8.2
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Sheep, Rabbit, Dog
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: GTF2H5; Polyclonal Antibody; GTF2H5 Antibody; anti-GTF2H5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Sheep, Rabbit, Dog
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GTF2H5 Antibody detects endogenous levels of GTF2H5.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 71

• Applicable Applications for anti-GTF2H5 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human GTF2H5.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Nucleus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Human kidney tissue, using GTF2H5 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-GTF2H5 antibody

Description: Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. Necessary for the stability of the TFIIH complex and for the presence of normal levels of TFIIH in the cell.

Subunit Structure: Component of the 7-subunit TFIIH core complex composed of XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and GTF2H5, which is active in NER. The core complex associates with the 3-subunit CDK-activating kinase (CAK) module composed of CCNH/cyclin H, CDK7 and MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription.

Similarity: Belongs to the TFB5 family.

NCBI and Uniprot Product Information

• NCBI GI #: 46359855
• NCBI GeneID: 404672
• NCBI Accession #: NP_997001.1
• NCBI GenBank Nucleotide #: NP_997001.1
• UniProt Accession #: Q6ZYL4
• Molecular Weight: Observed: 8 kDa; Predicted: 9 kDa
• NCBI Official Full Name: general transcription factor IIH subunit 5
• NCBI Official Synonym Full Names: general transcription factor IIH subunit 5
• NCBI Official Symbol: GTF2H5
• NCBI Official Synonym Symbols: TTD; TFB5; TTD3; TTDA; TFIIH; TTD-A; TGF2H5; C6orf175; bA120J8.2
• NCBI Protein Information: general transcription factor IIH subunit 5
• UniProt Protein Name: General transcription factor IIH subunit 5
• Protein Family: General transcription factor
• UniProt Gene Name: GTF2H5
• UniProt Synonym Gene Names: C6orf175; TTDA
• UniProt Entry Name: TF2H5_HUMAN

NCBI Description

This gene encodes a subunit of transcription/repair factor TFIIH, which functions in gene transcription and DNA repair. This protein stimulates ERCC3/XPB ATPase activity to trigger DNA opening during DNA repair, and is implicated in regulating cellular levels of TFIIH. Mutations in this gene result in trichothiodystrophy, complementation group A. [provided by RefSeq, Mar 2009]

Uniprot Description

GTF2H5: Component of the TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. Necessary for the stability of the TFIIH complex and for the presence of normal levels of TFIIH in the cell. Defects in GTF2H5 are a cause of trichothiodystrophy photosensitive (TTDP). TTDP is an autosomal recessive disease characterized by sulfur-deficient brittle hair and nails, ichthyosis, mental retardation, impaired sexual development, abnormal facies and cutaneous photosensitivity correlated with a nucleotide excision repair (NER) defect. Neonates with trichothiodystrophy and ichthyosis are usually born with a collodion membrane. The severity of the ichthyosis after the membrane is shed is variable, ranging from a mild to severe lamellar ichthyotic phenotype. There are no reports of skin cancer associated with TTDP. Belongs to the TFB5 family.

Protein type: DNA repair, damage; DNA-binding

Chromosomal Location of Human Ortholog: 6q25.3

Cellular Component: nucleoplasm; transcription factor TFIID complex

Biological Process: mRNA capping; nucleotide-excision repair; nucleotide-excision repair, DNA duplex unwinding; nucleotide-excision repair, DNA incision; nucleotide-excision repair, DNA incision, 3'-to lesion; nucleotide-excision repair, DNA incision, 5'-to lesion; nucleotide-excision repair, preincision complex assembly; nucleotide-excision repair, preincision complex stabilization; RNA elongation from RNA polymerase I promoter; RNA elongation from RNA polymerase II promoter; termination of RNA polymerase I transcription; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase I promoter; transcription initiation from RNA polymerase II promoter; transcription-coupled nucleotide-excision repair

Disease: Trichothiodystrophy 3, Photosensitive