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Western Blot (WB) (Western Blot analysis of GTF2F1 in A172 and HEPG2 cell lysates in order using GTF2F1 Rabbit pAb at dilution 1/250)

Rabbit GTF2F1 Polyclonal Antibody | anti-GTF2F1 antibody

GTF2F1 Rabbit pAb

Gene Names
GTF2F1; BTF4; RAP74; TF2F1; TFIIF
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
GTF2F1; Polyclonal Antibody; GTF2F1 Rabbit pAb; general transcription factor IIF subunit 1; BTF4; RAP74; TF2F1; TFIIF; anti-GTF2F1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Concentration
0.6mg/ml (varies by lot)
Applicable Applications for anti-GTF2F1 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:200-1:1000
IHC-P: 1:30-1:150
Immunogen
Fusion protein of human GTF2F1
Conjugation
Unconjugated
Modification
Unmodified
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western Blot analysis of GTF2F1 in A172 and HEPG2 cell lysates in order using GTF2F1 Rabbit pAb at dilution 1/250)

Western Blot (WB) (Western Blot analysis of GTF2F1 in A172 and HEPG2 cell lysates in order using GTF2F1 Rabbit pAb at dilution 1/250)

Immunohistochemistry (IHC)

(Immunohistochemistry of GTF2F1 in paraffin-embedded Human cervical cancer tissue using GTF2F1 Rabbit pAb at dilution 1/20. Predicted cell location: Nucleus and Cytoplasm)

Immunohistochemistry (IHC) (Immunohistochemistry of GTF2F1 in paraffin-embedded Human cervical cancer tissue using GTF2F1 Rabbit pAb at dilution 1/20. Predicted cell location: Nucleus and Cytoplasm)
Related Product Information for anti-GTF2F1 antibody
TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
517
NCBI Official Full Name
General transcription factor IIF subunit 1
NCBI Official Synonym Full Names
general transcription factor IIF, polypeptide 1, 74kDa
NCBI Official Symbol
GTF2F1
NCBI Official Synonym Symbols
BTF4; RAP74; TF2F1; TFIIF
NCBI Protein Information
general transcription factor IIF subunit 1; TFIIF-alpha; transcription initiation factor RAP74; general transcription factor IIF 74 kDa subunit; transcription initiation factor IIF subunit alpha
UniProt Protein Name
General transcription factor IIF subunit 1
UniProt Gene Name
GTF2F1
UniProt Synonym Gene Names
RAP74; TFIIF-alpha
UniProt Entry Name
T2FA_HUMAN

Uniprot Description

GTF2F1: TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. Heterodimer of an alpha and a beta subunit. Interacts with GTF2F2, CTDP1, TAF6/TAFII80 and URI1. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the TFIIF alpha subunit family.

Protein type: Kinase, protein; EC 2.7.11.1; Transcription initiation complex; ATYPICAL group; GTF2F1 family

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; transcription factor TFIIF complex; cell junction; nucleus

Molecular Function: protein binding; DNA binding; phosphatase activator activity; transcription coactivator activity; transcription factor binding; catalytic activity

Biological Process: positive regulation of catalytic activity; transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; transcription initiation from RNA polymerase II promoter; mRNA capping; viral reproduction; positive regulation of RNA elongation from RNA polymerase II promoter; positive regulation of viral transcription; RNA splicing; response to virus; RNA elongation from RNA polymerase II promoter; gene expression

Research Articles on GTF2F1

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Product Notes

The GTF2F1 gtf2f1 (Catalog #AAA4760318) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GTF2F1 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GTF2F1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:200-1:1000 IHC-P: 1:30-1:150. Researchers should empirically determine the suitability of the GTF2F1 gtf2f1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GTF2F1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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