Rabbit anti-Human GSTM5 Polyclonal Antibody | anti-GSTM5 antibody

GSTM5, NT (GSTM5, Glutathione S-transferase Mu 5, GST class-mu 5, GSTM5-5)

Cat. Num. AAA641815

• Gene Names: GSTM5; GTM5; GSTM5-5
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: GSTM5; Polyclonal Antibody; NT (GSTM5; Glutathione S-transferase Mu 5; GST class-mu 5; GSTM5-5); Anti -GSTM5; anti-GSTM5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-GSTM5 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50; Flow Cytometry: 1:10-50
• Immunogen: GSTM5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-51 amino acids from the N-terminal region of human GSTM5.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GSTM5 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the GSTM5 antibody detected the GSTM5 protein (arrow).)

Immunohistochemistry (IHC)

(GSTM5 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GSTM5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(GSTM5 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GSTM5 antibody

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Diversification of these genes has occurred in regions encoding substrate-binding domains, as well as in tissue expression patterns, to accommodate an increasing number of foreign compounds.

Product Categories/Family for anti-GSTM5 antibody

Antibodies; Abs to GST

NCBI and Uniprot Product Information

• NCBI GI #: 23065563
• NCBI GeneID: 2949
• NCBI Accession #: NP_000842.2
• NCBI GenBank Nucleotide #: NM_000851.3
• UniProt Accession #: P46439; Q6PD78; A8K0V8
• Molecular Weight: 25,675 Da
• NCBI Official Full Name: glutathione S-transferase Mu 5
• NCBI Official Synonym Full Names: glutathione S-transferase mu 5
• NCBI Official Symbol: GSTM5
• NCBI Official Synonym Symbols: GTM5; GSTM5-5
• NCBI Protein Information: glutathione S-transferase Mu 5; GST class-mu 5; glutathione S-transferase M5; glutathione S-aryltransferase M5; glutathione S-alkyltransferase M5; glutathione S-aralkyltransferase M5; S-(hydroxyalkyl)glutathione lyase M5
• UniProt Protein Name: Glutathione S-transferase Mu 5
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: GSTM5
• UniProt Entry Name: GSTM5_HUMAN

NCBI Description

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Diversification of these genes has occurred in regions encoding substrate-binding domains, as well as in tissue expression patterns, to accommodate an increasing number of foreign compounds. [provided by RefSeq, Jul 2008]

Uniprot Description

GSTM5: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Belongs to the GST superfamily. Mu family.

Protein type: Xenobiotic Metabolism - metabolism by cytochrome P450; Other Amino Acids Metabolism - glutathione; Transferase; EC 2.5.1.18; Xenobiotic Metabolism - drug metabolism - cytochrome P450

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: cytosol

Molecular Function: glutathione transferase activity

Biological Process: glutathione metabolic process; xenobiotic metabolic process