Immunohistochemistry (IHC)
(Anti-GST3/GST pi antibody, MBS175164, IHC(P)IHC(P): Human Intestinal Cancer Tissue )
Rabbit GST3/GST pi Polyclonal Antibody | anti-GSTP1 antibody
Anti-GST3/GST pi antibody
No cross reactivity with other proteins
No cross reactivity with other proteins
Concentration: 0.1-0.5ug/ml
Tested Species: Hu, Rat
Concluded Species: Ms
Antigen Retrieval: n/a
Immunohistochemistry (IHC-P):
Concentration: 0.5-1ug/ml
Tested Species: Hu
Concluded Species: n/a
Antigen Retrieval: By Heat
Other applications have not been tested. Optimal dilutions should be determined by end user.
Related detection systems:
Antibody can be supported by chemiluminescence kit MBS176460 in WB, supported by MBS176451 in IH(P).
Immunohistochemistry (IHC)
(Anti-GST3/GST pi antibody, MBS175164, IHC(P)IHC(P): Human Intestinal Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-GST3/GST pi antibody, MBS175164, IHC(P)IHC(P): Human Intestinal Cancer Tissue )
Western Blot (WB)
(Anti-GST3/GST pi antibody, MBS175164,Western blottingAll lanes: Anti GST3/GST pi (MBS175164) at 0.5ug/mlLane 1: Rat Kidney Tissue Lysate at 50ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 23KDObserved bind size: 23KD )
Western Blot (WB)
(Anti-GST3/GST pi antibody, MBS175164,Western blottingAll lanes: Anti GST3/GST pi (MBS175164) at 0.5ug/mlLane 1: Rat Kidney Tissue Lysate at 50ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 23KDObserved bind size: 23KD )
Background: Glutathione S-transferases pi, also known as GST3, present in all tissues and cells, with the exception of red cells, in which only erythrocyte GST(GSTe) is observed. The GST-pi gene has 7 exons and 6 introns contained within approximately 2.8 kilobases. The GST-pi gene is mapped to chromosome 11. Placental glutathione-S-transferase-pi mRNA is abundantly expressed in human skin. GSTP does not contribute in vivo to the formation of glutathione conjugates of acetaminophen but plays a novel and unexpected role in the toxicity of this compound.
NCBI and Uniprot Product Information
NCBI Description
Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq, Jul 2008]
Uniprot Description
GSTP1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration. Homodimer. Interacts with CDK5. Belongs to the GST superfamily. Pi family.
Protein type: Transferase; EC 2.5.1.18; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450; Other Amino Acids Metabolism - glutathione
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: extracellular space; mitochondrion; cytoplasm; plasma membrane; intracellular; cytosol; nucleus; vesicle
Molecular Function: JUN kinase binding; protein binding; glutathione transferase activity; kinase regulator activity
Biological Process: negative regulation of MAP kinase activity; negative regulation of JNK activity; central nervous system development; negative regulation of MAPKKK cascade; negative regulation of acute inflammatory response; negative regulation of tumor necrosis factor production; negative regulation of interleukin-1 beta production; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of stress-activated MAPK cascade; regulation of stress-activated MAPK cascade; negative regulation of biosynthetic process; response to reactive oxygen species; glutathione metabolic process; negative regulation of nitric-oxide synthase biosynthetic process; negative regulation of fibroblast proliferation; positive regulation of superoxide release; xenobiotic metabolic process; negative regulation of protein kinase activity; negative regulation of apoptosis
Research Articles on GSTP1
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Product Notes
The GSTP1 gstp1 (Catalog #AAA175164) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GST3/GST pi antibody reacts with Human, Mouse, Rat No cross reactivity with other proteins and may cross-react with other species as described in the data sheet. AAA Biotech's GST3/GST pi can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot (WB): Concentration: 0.1-0.5ug/ml Tested Species: Hu, Rat Concluded Species: Ms Antigen Retrieval: Immunohistochemistry (IHC-P): Concentration: 0.5-1ug/ml Tested Species: Hu Concluded Species: Antigen Retrieval: By Heat Other applications have not been tested. Optimal dilutions should be determined by end user. Related detection systems: Antibody can be supported by chemiluminescence kit MBS176460 in WB, supported by MBS176451 in IH(P). Researchers should empirically determine the suitability of the GSTP1 gstp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GST3/GST pi, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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