Rabbit anti-Human GSR Polyclonal Antibody | anti-GSR antibody

GSR, CT (GSR, GLUR, GRD1, Glutathione reductase, mitochondrial) (FITC)

Cat. Num. AAA6304682

• Gene Names: GSR; HEL-75
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GSR; Polyclonal Antibody; CT (GSR; GLUR; GRD1; Glutathione reductase; mitochondrial) (FITC); mitochondrial; anti-GSR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-GSR antibody: Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IF: 1:10-50; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GSR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 387-416 amino acids from the C-terminal region of human GSR.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GSR Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the GSR antibody detected the GSR protein (arrow).)

Immunohistochemistry (IHC)

(GSR Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GSR Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of GSR Antibody (C-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(GSR Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GSR antibody

This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found.

Product Categories/Family for anti-GSR antibody

Antibodies; Mitochondrial Proteins

References

Satoh, N., et al. Biochem. Genet. 48 (9-10), 816-821 (2010): Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Wang, Y., et al. J. Hum. Genet. 55(8):490-494(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Moyer, A.M., et al. Cancer Epidemiol. Biomarkers Prev. 19(3):811-821(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 50301238
• NCBI GeneID: 2936
• NCBI Accession #: NP_000628.2
• NCBI GenBank Nucleotide #: NM_000637.3
• UniProt Accession #: P00390; Q7Z5C9; Q9NP63; C8KIL8; C8KIL9; C8KIM0; D3DSV3
• Molecular Weight: 47,267 Da
• NCBI Official Full Name: glutathione reductase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: glutathione reductase
• NCBI Official Symbol: GSR
• NCBI Official Synonym Symbols: HEL-75
• NCBI Protein Information: glutathione reductase, mitochondrial; GR; GRase; epididymis luminal protein 75
• UniProt Protein Name: Glutathione reductase, mitochondrial
• Protein Family: Glutathione reductase
• UniProt Gene Name: GSR
• UniProt Synonym Gene Names: GLUR; GRD1; GR; GRase
• UniProt Entry Name: GSHR_HUMAN

NCBI Description

This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2010]

Uniprot Description

GSR: a class-I pyridine nucleotide-disulfide oxidoreductase. Maintains high levels of reduced glutathione in the cytosol. Alternative initiation results in a mitochondrial and a cytoplasmic isoform.

Protein type: EC 1.8.1.7; Other Amino Acids Metabolism - glutathione; Oxidoreductase

Chromosomal Location of Human Ortholog: 8p21.1

Cellular Component: mitochondrial matrix; cytosol; external side of plasma membrane

Molecular Function: mercury (II) reductase activity; FAD binding; electron carrier activity; mercury ion binding; NADP binding; glutathione-disulfide reductase activity

Biological Process: response to reactive oxygen species; glutathione metabolic process; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; cell redox homeostasis; detoxification of mercury ion