Rabbit anti-Human, Mouse GSDMA Polyclonal Antibody | anti-GSDMA antibody

GSDMA, NT (GSDMA, GSDM, GSDM1, Gasdermin-A, Gasdermin-1) (Azide free) (HRP)

Cat. Num. AAA6304617

• Gene Names: GSDMA; GSDM; FKSG9; GSDM1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GSDMA; Polyclonal Antibody; NT (GSDMA; GSDM; GSDM1; Gasdermin-A; Gasdermin-1) (Azide free) (HRP); Gasdermin-1; anti-GSDMA antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GSDMA. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-GSDMA antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 66-94 in the N-terminal region of human GSDMA, UniProt Accession #Q96OA5.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of mouse liver tissue lysates (35ug/lane) usingMBS642685. GSDMA (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of MBS642685 in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of 293 cells (right histogram) using MBS642685 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-GSDMA antibody

Antibodies; Proteins

References

Soranzo, N., et al. Nat. Genet. 41(11):1182-1190(2009). Saeki, N., et al. Genes Chromosomes Cancer 48(3):261-271(2009). Tamura, M., et al. Genomics 89(5):618-629(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 150456463
• NCBI GeneID: 284110
• NCBI Accession #: NP_835465.2
• NCBI GenBank Nucleotide #: NM_178171.4
• UniProt Accession #: Q96QA5; Q32MC5; Q86VE7; Q8N1M6
• Molecular Weight: 49,365 Da
• NCBI Official Full Name: gasdermin-A
• NCBI Official Synonym Full Names: gasdermin A
• NCBI Official Symbol: GSDMA
• NCBI Official Synonym Symbols: GSDM; FKSG9; GSDM1
• NCBI Protein Information: gasdermin-A; gasdermin 1; gasdermin-1
• UniProt Protein Name: Gasdermin-A
• Protein Family: Gasdermin
• UniProt Gene Name: GSDMA
• UniProt Synonym Gene Names: GSDM; GSDM1
• UniProt Entry Name: GSDMA_HUMAN

Uniprot Description

GSDMA: Induces apoptosis. Belongs to the gasdermin family.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 17q21.1

Cellular Component: perinuclear region of cytoplasm

Biological Process: apoptosis