Western Blot (WB)
(Anti-GRP94 antibody, MBS175715, Western blottingLane 1: RAJI Cell LysateLane 2: MCF-7 Cell Lysate )
Rabbit GRP94 Polyclonal Antibody | anti-HSP90B1 antibody
Anti-GRP94 antibody
Western Blot (WB)
(Anti-GRP94 antibody, MBS175715, Western blottingLane 1: RAJI Cell LysateLane 2: MCF-7 Cell Lysate )
Western Blot (WB)
(Anti-GRP94 antibody, MBS175715, Western blottingLane 1: RAJI Cell LysateLane 2: MCF-7 Cell Lysate )
Immunohistochemistry (IHC)
(Anti-GRP94 antibody, MBS175715, IHC(P)IHC(P): HELA Cell )
Immunohistochemistry (IHC)
(Anti-GRP94 antibody, MBS175715, IHC(P)IHC(P): HELA Cell )
Immunohistochemistry (IHC)
(Anti-GRP94 antibody, MBS175715, IHC(P)IHC(P): Human Lung Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-GRP94 antibody, MBS175715, IHC(P)IHC(P): Human Lung Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-GRP94 antibody, MBS175715, IHC(P)IHC(P): Rat Brain Tissue )
Immunohistochemistry (IHC)
(Anti-GRP94 antibody, MBS175715, IHC(P)IHC(P): Rat Brain Tissue )
Western Blot (WB)
(Applications: Western BlotSample: Cell lysateDetection: Enhanced chemiluminescence"This is an excellent antibody to endoplasmin in HC11 cells. Clean, reliable detection with very little if any background. Great antibody for Westerns. Could probably be optimized for 1 hour primary incubation.")
Western Blot (WB)
(Applications: Western BlotSample: Cell lysateDetection: Enhanced chemiluminescence"This is an excellent antibody to endoplasmin in HC11 cells. Clean, reliable detection with very little if any background. Great antibody for Westerns. Could probably be optimized for 1 hour primary incubation.")
Background: Heat shock protein HSP 90-beta is a protein that in humans is encoded by the HSP90AB1 gene. Hsp90beta is mapped to chromosome 12q23.3. The function of Hsp90betaincludes assisting in protein folding, cell signaling, and tumor repression. This protein was first isolated by extracting proteins from stressed cells. These cells were stressed by heating, dehydrating or by other means, all of which caused the cell's proteins to begin to denature.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012]
Uniprot Description
GRP94: Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with OS9. Interacts with CNPY3. This interaction is disrupted in the presence of ATP. Interacts with TLR4 and TLR9, but not with TLR3. Belongs to the heat shock protein 90 family.
Protein type: Secreted, signal peptide; Apoptosis; Secreted; Heat shock protein
Chromosomal Location of Human Ortholog: 12q24.2-q24.3
Cellular Component: extracellular matrix; endoplasmic reticulum membrane; focal adhesion; membrane; endoplasmic reticulum lumen; perinuclear region of cytoplasm; endoplasmic reticulum; melanosome; plasma membrane; extracellular region; midbody; nucleus; cytosol
Molecular Function: protein binding; low-density lipoprotein receptor binding; RNA binding; unfolded protein binding; virion binding; calcium ion binding; protein phosphatase binding; ATP binding
Biological Process: actin rod formation; receptor-mediated endocytosis; ER-associated protein catabolic process; protein transport; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; unfolded protein response; sequestering of calcium ion; toll-like receptor signaling pathway; response to hypoxia; innate immune response; regulation of phosphoprotein phosphatase activity; negative regulation of apoptosis
Research Articles on HSP90B1
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Product Notes
The HSP90B1 hsp90b1 (Catalog #AAA175715) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GRP94 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GRP94 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry ICC. Researchers should empirically determine the suitability of the HSP90B1 hsp90b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GRP94, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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