Rabbit GRP78 Polyclonal Antibody | anti-Hspa5 antibody

GRP78 Antibody: FITC

Cat. Num. AAA806132

• Gene Names: Hspa5; BIP; GRP78
• Reactivity: Human (weak), Mouse, Rat
• Applications: Western Blot
• Synonyms: GRP78; Polyclonal Antibody; GRP78 Antibody: FITC; BIP; Grp78; HspA5; MIF2; immunoglobulin heavy chain binding protein; anti-Hspa5 antibody

• Host: Rabbit
• Reactivity: Human (weak), Mouse, Rat
• Clonality: Polyclonal
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 654

• Applicable Applications for anti-Hspa5 antibody: Western Blot (WB)
• Application Notes: 1:1000 (WB)
• Conjugate: FITC
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP78 Polyclonal Antibody . Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody at 1:60 for 12 hours at 4 degree C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP78 Antibody. (C) Composite. Heat Shocked at 42 degree C for 1h.)

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP78 Polyclonal Antibody . Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody at 1:60 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP78 Antibody. (C) Composite. Heat Shocked at 42 degree C for 1h.)

Western Blot (WB)

(Western blot analysis of Rat Brain cell lysates showing detection of ~78 kDa GRP78 protein using Rabbit Anti-GRP78 Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~78 kDa.)

Related Product Information for anti-Hspa5 antibody

Background Info: Detects a 78kDa protein corresponding to the molecular mass of Grp78 in SDS PAGE immunoblots.

Scientific Background: GRP78 is a ubiquitously expressed, 78-kDa glucose-regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' nontranslated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer's patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti¬tumor drugs (6, 7).

Product Categories/Family for anti-Hspa5 antibody

Chaperones; Heat Shock; Organelle Markers; Trafficking

References

1. Cho S., et al. (2007). Mol Cell Biol 27(1): 368-83. 2. Yang Y., et al. (1998) J Biol Chem 273: 25552-25555. 3. Luo S., et al (2006) 26 (15): 5688-97. 4. Yu Z., et al. (1999) Exp Neurol. 15: 302-314. 5. Koomagi R., et al. (1999) Anticancer Res. 19:4333- 4336. 6. Laquerre S., et al. (1998) J. Virology 72: 4940-4949. 7. Dong D., et al. (2005) Cancer Res 65(13): 5785-91.

NCBI and Uniprot Product Information

• NCBI GI #: 25742763
• NCBI GeneID: 25617
• NCBI Accession #: NP_037215.1
• NCBI GenBank Nucleotide #: NM_013083.2
• UniProt Accession #: P06761
• Molecular Weight: 72,347 Da
• NCBI Official Full Name: 78 kDa glucose-regulated protein
• NCBI Official Synonym Full Names: heat shock protein 5
• NCBI Official Symbol: Hspa5
• NCBI Official Synonym Symbols: BIP; GRP78
• NCBI Protein Information: 78 kDa glucose-regulated protein; GRP 78; GRP-78; heat shock 70kD protein 5; heat shock 70 kDa protein 5; steroidogenesis-activator polypeptide; immunoglobulin heavy chain-binding protein; heat shock 70kDa protein 5 (glucose-regulated protein)
• UniProt Protein Name: 78 kDa glucose-regulated protein
• UniProt Gene Name: Hspa5
• UniProt Synonym Gene Names: Grp78; GRP-78; BiP
• UniProt Entry Name: GRP78_RAT

NCBI Description

secreted protein of the endoplasmic reticulum; may be involved in the assembly of secreted and membrane-bound proteins [RGD, Feb 2006]

Uniprot Description

GRP78: a member of the HSP family of molecular chaperones required for endoplasmic reticulum integrity and stress-induced autophagy. Plays a central role in regulating the unfolded protein response (UPR), and is an obligatory component of autophagy in mammalian cells. May play an important role in cellular adaptation and oncogenic survival. One of the client proteins of GRP78 is protein double-stranded RNA-activated protein-like endoplasmic reticulum kinase (PERK). Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.

Protein type: Chaperone; Heat shock protein

Cellular Component: signalosome; endoplasmic reticulum membrane; focal adhesion; cell surface; smooth endoplasmic reticulum; mitochondrion; endoplasmic reticulum; endoplasmic reticulum lumen; ER-Golgi intermediate compartment; membrane; plasma membrane; melanosome; integral to endoplasmic reticulum membrane; midbody; nucleus; myelin sheath

Molecular Function: protein domain specific binding; protein binding; enzyme binding; ubiquitin protein ligase binding; unfolded protein binding; ribosome binding; misfolded protein binding; glycoprotein binding; ATP binding

Biological Process: cellular response to glucose starvation; unfolded protein response, activation of signaling protein activity; substantia nigra development; cerebellum structural organization; proteolysis involved in cellular protein catabolic process; positive regulation of protein ubiquitination; cerebellar Purkinje cell layer development; negative regulation of transforming growth factor beta receptor signaling pathway; ER overload response; negative regulation of apoptosis; positive regulation of cell migration