Rabbit GRK5 Polyclonal Antibody | anti-GRK5 antibody

GRK5 Antibody (C-term)

Cat. Num. AAA9214704

• Gene Names: GRK5; GPRK5
• Reactivity: Human, mouse (Predicted Reactivity: Bovine)
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: GRK5; Polyclonal Antibody; GRK5 Antibody (C-term); G protein-coupled receptor kinase 5; G protein-coupled receptor kinase GRK5; GPRK5; anti-GRK5 antibody

• Host: Rabbit
• Reactivity: Human, mouse (Predicted Reactivity: Bovine)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This GRK5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 559-590 amino acids from the C-terminal region of human GRK5.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Sequence Positions: 559-590
• Sequence Length: 590

• Applicable Applications for anti-GRK5 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-GRK5 Antibody (N574) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: HepG2 whole cell lysatesLane 3: Ramos whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 68 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(All lanes : Anti-GRK5 Antibody (N574) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: HepG2 whole cell lysatesLane 3: Ramos whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 68 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Western blot analysis of anti-GRK5 Pab in mouse brain tissue lysate. GRK5 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GRK5 Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-GRK5 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).

Product Categories/Family for anti-GRK5 antibody

Cardiovascular; Signal Transduction

References

Pronin, A.N., et al., J. Biol. Chem. 275(34):26515-26522 (2000).
Pronin, A.N., et al., J. Biol. Chem. 273(47):31510-31518 (1998).
Nagayama, Y., et al., J. Biol. Chem. 271(17):10143-10148 (1996).
Kunapuli, P., et al., J. Biol. Chem. 269(2):1099-1105 (1994).
Kunapuli, P., et al., Proc. Natl. Acad. Sci. U.S.A. 90(12):5588-5592 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 4885349
• NCBI GeneID: 2869
• NCBI Accession #: NP_005299.1
• NCBI GenBank Nucleotide #: NM_005308.2
• UniProt Accession #: P34947; Q5T059; D3DRD0
• Molecular Weight: 67787
• NCBI Official Full Name: G protein-coupled receptor kinase 5
• NCBI Official Synonym Full Names: G protein-coupled receptor kinase 5
• NCBI Official Symbol: GRK5
• NCBI Official Synonym Symbols: GPRK5
• NCBI Protein Information: G protein-coupled receptor kinase 5
• UniProt Protein Name: G protein-coupled receptor kinase 5
• Protein Family: G protein-coupled receptor kinase
• UniProt Gene Name: GRK5
• UniProt Synonym Gene Names: GPRK5
• UniProt Entry Name: GRK5_HUMAN

NCBI Description

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear leukocytes (PMNs). [provided by RefSeq, Jul 2008]

Uniprot Description

GRK5: Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein- coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70- interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2- mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G- protein-coupled receptor, LRP6 during Wnt signaling (in vitro). Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Protein type: Kinase, protein; EC 2.7.11.16; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); AGC group; GRK family; GRK subfamily

Chromosomal Location of Human Ortholog: 10q26.11

Cellular Component: nucleoplasm; nuclear membrane; focal adhesion; cytoplasm; plasma membrane; nucleus

Molecular Function: G-protein coupled receptor kinase activity; protein serine/threonine kinase activity; protein binding; protein kinase C binding; phospholipid binding; ATP binding

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; tachykinin signaling pathway; Wnt receptor signaling pathway; apoptosis; protein amino acid autophosphorylation; regulation of G-protein coupled receptor protein signaling pathway; negative regulation of apoptosis