Rabbit anti-Human, Mouse GRK5 Polyclonal Antibody | anti-GRK5 antibody

GRK5, CT (G-Protein Coupled Receptor Kinase 5, G-Protein-coupled Receptor Kinase GRK5, GPRK5, FLJ39780) (APC)

Cat. Num. AAA6479013

• Gene Names: GRK5; GPRK5
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: GRK5; Polyclonal Antibody; CT (G-Protein Coupled Receptor Kinase 5; G-Protein-coupled Receptor Kinase GRK5; GPRK5; FLJ39780) (APC); EC=2.7.11.16; anti-GRK5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GRK5. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-GRK5 antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 559-590 in the C-terminal region of human GRK5. Species sequence homology: bovine
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse brain tissue lysate usingMBS623728. GRK5 (arrow) was detected usingMBS623728. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Testing Data

(All lanes: MBS623728 (1:2000) Lane 1: Hela whole cell lysates Lane 2: HepG2 whole cell lysates Lane 3: Ramos whole cell lysates Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 68kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human cancer tissue reacted with theMBS623728, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS623728 for immunohistochemistry. BC = breast carcinoma)

Product Categories/Family for anti-GRK5 antibody

Antibodies; G Protein Receptors

References

1. Pronin, A.N., et al., J. Biol. Chem. 275(34):26515-26522 (2000). 2. Pronin, A.N., et al., J. Biol. Chem. 273(47):31510-31518 (1998). 3. Nagayama, Y., et al., J. Biol. Chem. 271(17):10143-10148 (1996). 4. Kunapuli, P., et al., J. Biol. Chem. 269(2):1099-1105 (1994). 5. Kunapuli, P., et al., Proc. Natl. Acad. Sci. USA 90(12):5588-5592 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 4885349
• NCBI GeneID: 2869
• NCBI Accession #: NP_005299.1
• NCBI GenBank Nucleotide #: NM_005308.2
• UniProt Accession #: P34947; Q5T059; D3DRD0
• Molecular Weight: 67,787 Da
• NCBI Official Full Name: G protein-coupled receptor kinase 5
• NCBI Official Synonym Full Names: G protein-coupled receptor kinase 5
• NCBI Official Symbol: GRK5
• NCBI Official Synonym Symbols: GPRK5
• NCBI Protein Information: G protein-coupled receptor kinase 5; FP2025; g protein-coupled receptor kinase GRK5
• UniProt Protein Name: G protein-coupled receptor kinase 5
• Protein Family: G protein-coupled receptor kinase
• UniProt Gene Name: GRK5
• UniProt Synonym Gene Names: GPRK5
• UniProt Entry Name: GRK5_HUMAN

NCBI Description

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear leukocytes (PMNs). [provided by RefSeq, Jul 2008]

Uniprot Description

GRK5: Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein- coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70- interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2- mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G- protein-coupled receptor, LRP6 during Wnt signaling (in vitro). Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; EC 2.7.11.16; Kinase, protein; AGC group; GRK family; GRK subfamily

Chromosomal Location of Human Ortholog: 10q26.11

Cellular Component: nucleoplasm; nuclear membrane; focal adhesion; cytoplasm; plasma membrane; nucleus

Molecular Function: G-protein coupled receptor kinase activity; protein serine/threonine kinase activity; protein binding; protein kinase C binding; phospholipid binding; ATP binding

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; tachykinin signaling pathway; Wnt receptor signaling pathway; apoptosis; protein amino acid autophosphorylation; regulation of G-protein coupled receptor protein signaling pathway; negative regulation of apoptosis