Highly validated and characterized monoclonal/polyclonal
antibodies and recombinant
proteins
The majority of AAA Biotech’s antibodies are highly validated and can be use in multiple
applications such as ELISA, FC,
ICC, IF, IHC, IP, WB, etc. We have antibodies available for rare species, in multiple conjugated
forms or recombinant
antibodies.
As for our high quality proteins, the majority have 90% purity, detected by SDS-PAGE while some are
available in
different tags such as Flag, GST, His, MBP, etc. We also carry high quality native and biologically
active proteins.
AAA Biotech is constantly working to expand our capacity to provide
recombinant proteins, antibodies and ELISA kits to most target proteins.
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FROM (`products`, `products` as `p`)
LEFT OUTER JOIN `products_description` as `pd` ON `p`.`products_id` = `pd`.`products_id`
LEFT OUTER JOIN `products_description_ncbi_sp` as `pdns` ON `p`.`products_id` = `pdns`.`products_id`
LEFT OUTER JOIN `products_extra` as `pe` ON `p`.`products_id` = `pe`.`products_id`
WHERE `p`.`products_id` = '21568'
AND `pd`.`language_id` = 1
LIMIT 1
Query
Database
1.77 ms
select p.*, pd.*,
ifnull(pdns.ncbi_summary, 'N/A') as ncbi_summary_pdns,
ifnull(pdns.sp_comments, 'N/A') as sp_comments_pdns,
ifnull(pdns.ncbi_research_articles, 'N/A') as ncbi_research_articles_pdns,
ifnull(pe.products_description_extra, 'N/A') as products_description_extra
from products p
LEFT OUTER JOIN products_description pd on p.products_id = pd.products_id
LEFT OUTER JOIN products_description_ncbi_sp pdns on p.products_id = pdns.products_id
LEFT OUTER JOIN products_extra pe on p.products_id = pe.products_id
where p.products_id = '21568' and pd.language_id = 1
Query
Database
1.49 ms
SELECT `options_values_price` as `price`, `products_options_values_name` as `package`
FROM `products_attributes`
JOIN `products_options_values` ON `products_options_values`.`products_options_values_id` = `products_attributes`.`options_values_id`
WHERE `products_attributes`.`products_id` = '21568'
Query
Database
9.23 ms
UPDATE `products_description` SET `products_viewed` = products_viewed + 1
WHERE `products_id` = 21568
Database (5 total Queries, 5 of them unique across 2 Connections)
Time
Query String
2.44 ms
SELECT `p`.*, `pd`.*, IFNULL(pdns.ncbi_summary, "N/A") as ncbi_summary_pdns, IFNULL(pdns.sp_comments, "N/A") as sp_comments_pdns, IFNULL(pdns.ncbi_research_articles, "N/A") as ncbi_research_articles_pdns, IFNULL(pe.products_description_extra, "N/A") as products_description_extra
FROM (`products`, `products` as `p`)
LEFT OUTER JOIN `products_description` as `pd` ON `p`.`products_id` = `pd`.`products_id`
LEFT OUTER JOIN `products_description_ncbi_sp` as `pdns` ON `p`.`products_id` = `pdns`.`products_id`
LEFT OUTER JOIN `products_extra` as `pe` ON `p`.`products_id` = `pe`.`products_id`
WHERE `p`.`products_id` = '21568'
AND `pd`.`language_id` = 1
LIMIT 1
select p.*, pd.*,
ifnull(pdns.ncbi_summary, 'N/A') as ncbi_summary_pdns,
ifnull(pdns.sp_comments, 'N/A') as sp_comments_pdns,
ifnull(pdns.ncbi_research_articles, 'N/A') as ncbi_research_articles_pdns,
ifnull(pe.products_description_extra, 'N/A') as products_description_extra
from products p
LEFT OUTER JOIN products_description pd on p.products_id = pd.products_id
LEFT OUTER JOIN products_description_ncbi_sp pdns on p.products_id = pdns.products_id
LEFT OUTER JOIN products_extra pe on p.products_id = pe.products_id
where p.products_id = '21568' and pd.language_id = 1
SELECT `options_values_price` as `price`, `products_options_values_name` as `package`
FROM `products_attributes`
JOIN `products_options_values` ON `products_options_values`.`products_options_values_id` = `products_attributes`.`options_values_id`
WHERE `products_attributes`.`products_id` = '21568'
⇄⧉etc_term1 => string (140) "Samples||Serum, plasma and other biological fluids!!Assay Type||Quantitative...
$value['etc_term1']
Samples||Serum, plasma and other biological fluids!!Assay Type||Quantitative Sandwich!!Detection Range||0.63-40ng/mL!!Sensitivity||0.38ng/mL
⇄⧉etc_term2 => string (370) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): 3 ...
$value['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat GLUT4 were tested 20 times on one plate, respectively.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat GLUT4 were tested on 3 different plates, 20 replicates in each plate.
⇄⧉products_description => string (1207) "Intended Uses: This ELISA kit applies to the in vitro quantitative determina...
$value['products_description']
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat GLUT4 concentrations in serum, plasma and other biological fluids.<br><br>Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GLUT4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GLUT4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GLUT4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat GLUT4. You can calculate the concentration of Rat GLUT4 in the samples by comparing the OD of the samples to the standard curve.
⇄products_references => string (3) "N/A"
$value['products_references']
⇄products_related_diseases => string (3) "N/A"
$value['products_related_diseases']
⇄products_categories => string (3) "N/A"
$value['products_categories']
⇄products_search_terms => string (3) "N/A"
$value['products_search_terms']
⇄ncbi_full_name => string (14) "glut4, partial"
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⇄ncbi_full_name_syn => string (3) "N/A"
$value['ncbi_full_name_syn']
⇄ncbi_symbol => string (3) "N/A"
$value['ncbi_symbol']
⇄ncbi_symbol_syn => string (3) "N/A"
$value['ncbi_symbol_syn']
⇄ncbi_protein_info => string (3) "N/A"
$value['ncbi_protein_info']
⇄ncbi_chrom_loc => string (3) "N/A"
$value['ncbi_chrom_loc']
⇄ncbi_gene_id => string (3) "N/A"
$value['ncbi_gene_id']
⇄ncbi_mol_weight => string (9) "20,643 Da"
$value['ncbi_mol_weight']
⇄ncbi_pathways => string (3) "N/A"
$value['ncbi_pathways']
⇄sp_protein_name => string (5) "Glut4"
$value['sp_protein_name']
⇄sp_protein_name_syn => string (5) "Glut4"
$value['sp_protein_name_syn']
⇄sp_gene_name => string (3) "N/A"
$value['sp_gene_name']
⇄sp_gene_name_syn => string (3) "N/A"
$value['sp_gene_name_syn']
⇄sp_entry_name => string (12) "Q58HK0_SHEEP"
$value['sp_entry_name']
⇄sp_mim => string (3) "N/A"
$value['sp_mim']
⇄sp_interactions => string (3) "N/A"
$value['sp_interactions']
⇄products_url => string (3) "N/A"
$value['products_url']
⇄products_viewed => string (1) "0"
$value['products_viewed']
⇄ncbi_summary_pdns => string (3) "N/A"
$value['ncbi_summary_pdns']
⇄sp_comments_pdns => string (3) "N/A"
$value['sp_comments_pdns']
⇄ncbi_research_articles_pdns => string (3) "N/A"
$value['ncbi_research_articles_pdns']
⇄products_description_extra => string (3) "N/A"
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title
Rat GLUT4 ELISA Kit | Glut4 elisa kit
datasheet
⧉⌕$value App\Libraries\DatasheetLibrary (10)
Properties (10)
Available methods (45)
⇄⧉public a -> array (75)
$value->a
⇄products_id_mbs => string (7) "2505083"
$value->a['products_id_mbs']
⇄products_id => string (5) "21568"
$value->a['products_id']
⇄products_quantity => string (4) "9999"
$value->a['products_quantity']
⇄products_model => string (8) "AAA21568"
$value->a['products_model']
⇄products_model_oem => string (10) "E-EL-R0430"
$value->a['products_model_oem']
⇄products_similar => string (3) "N/A"
$value->a['products_similar']
⇄ncbi_gi_num => string (8) "61661420"
$value->a['ncbi_gi_num']
⇄ncbi_acc_num => string (10) "AAX51300.1"
$value->a['ncbi_acc_num']
⇄ncbi_acc_num_related => string (3) "N/A"
$value->a['ncbi_acc_num_related']
⇄ncbi_sp_acc_num_related => string (3) "N/A"
$value->a['ncbi_sp_acc_num_related']
⇄ncbi_gb_acc_num => string (3) "N/A"
$value->a['ncbi_gb_acc_num']
⇄sp_acc_num => string (3) "N/A"
$value->a['sp_acc_num']
⇄sp_acc_num_syn => string (3) "N/A"
$value->a['sp_acc_num_syn']
⇄products_image => string (9) "ELISA Kit"
$value->a['products_image']
⇄sequence_positions => string (3) "N/A"
$value->a['sequence_positions']
⇄sequence_length => string (3) "190"
$value->a['sequence_length']
⇄sequence => string (3) "N/A"
$value->a['sequence']
⇄clonality => string (3) "N/A"
$value->a['clonality']
⇄isotype => string (3) "N/A"
$value->a['isotype']
⇄clone_number => string (3) "N/A"
$value->a['clone_number']
⇄host => string (3) "N/A"
$value->a['host']
⇄reactivity => string (3) "Rat"
$value->a['reactivity']
⇄⧉specificity => string (135) "This kit recognizes Rat GLUT4 in samples. No significant cross-reactivity or...
$value->a['specificity']
This kit recognizes Rat GLUT4 in samples. No significant cross-reactivity or interference between Rat GLUT4 and analogues was observed.
⇄purity => string (3) "N/A"
$value->a['purity']
⇄form => string (3) "N/A"
$value->a['form']
⇄concentration => string (3) "N/A"
$value->a['concentration']
⇄storage_stability => string (20) "Store at 4 degree C."
⇄⧉etc_term1 => string (140) "Samples||Serum, plasma and other biological fluids!!Assay Type||Quantitative...
$value->a['etc_term1']
Samples||Serum, plasma and other biological fluids!!Assay Type||Quantitative Sandwich!!Detection Range||0.63-40ng/mL!!Sensitivity||0.38ng/mL
⇄⧉etc_term2 => string (370) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): 3 ...
$value->a['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat GLUT4 were tested 20 times on one plate, respectively.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat GLUT4 were tested on 3 different plates, 20 replicates in each plate.
⇄⧉products_description => string (1207) "Intended Uses: This ELISA kit applies to the in vitro quantitative determina...
$value->a['products_description']
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat GLUT4 concentrations in serum, plasma and other biological fluids.<br><br>Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GLUT4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GLUT4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GLUT4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat GLUT4. You can calculate the concentration of Rat GLUT4 in the samples by comparing the OD of the samples to the standard curve.
⇄products_references => string (3) "N/A"
$value->a['products_references']
⇄products_related_diseases => string (3) "N/A"
$value->a['products_related_diseases']
⇄products_categories => string (3) "N/A"
$value->a['products_categories']
⇄products_search_terms => string (3) "N/A"
$value->a['products_search_terms']
⇄ncbi_full_name => string (14) "glut4, partial"
$value->a['ncbi_full_name']
⇄ncbi_full_name_syn => string (3) "N/A"
$value->a['ncbi_full_name_syn']
⇄ncbi_symbol => string (3) "N/A"
$value->a['ncbi_symbol']
⇄ncbi_symbol_syn => string (3) "N/A"
$value->a['ncbi_symbol_syn']
⇄ncbi_protein_info => string (3) "N/A"
$value->a['ncbi_protein_info']
⇄ncbi_chrom_loc => string (3) "N/A"
$value->a['ncbi_chrom_loc']
⇄ncbi_gene_id => string (3) "N/A"
$value->a['ncbi_gene_id']
⇄ncbi_mol_weight => string (9) "20,643 Da"
$value->a['ncbi_mol_weight']
⇄ncbi_pathways => string (3) "N/A"
$value->a['ncbi_pathways']
⇄sp_protein_name => string (5) "Glut4"
$value->a['sp_protein_name']
⇄sp_protein_name_syn => string (5) "Glut4"
$value->a['sp_protein_name_syn']
⇄sp_gene_name => string (3) "N/A"
$value->a['sp_gene_name']
⇄sp_gene_name_syn => string (3) "N/A"
$value->a['sp_gene_name_syn']
⇄sp_entry_name => string (12) "Q58HK0_SHEEP"
$value->a['sp_entry_name']
⇄sp_mim => string (3) "N/A"
$value->a['sp_mim']
⇄sp_interactions => string (3) "N/A"
$value->a['sp_interactions']
⇄products_url => string (3) "N/A"
$value->a['products_url']
⇄products_viewed => string (1) "0"
$value->a['products_viewed']
⇄ncbi_summary_pdns => string (3) "N/A"
$value->a['ncbi_summary_pdns']
⇄sp_comments_pdns => string (3) "N/A"
$value->a['sp_comments_pdns']
⇄ncbi_research_articles_pdns => string (3) "N/A"
$value->a['ncbi_research_articles_pdns']
⇄products_description_extra => string (3) "N/A"
$value->a['products_description_extra']
⇄⧉public d -> array (75)
$value->d
⇄products_id_mbs => string (7) "2505083"
$value->d['products_id_mbs']
⇄products_id => string (5) "21568"
$value->d['products_id']
⇄products_quantity => string (4) "9999"
$value->d['products_quantity']
⇄products_model => string (8) "AAA21568"
$value->d['products_model']
⇄products_model_oem => string (10) "E-EL-R0430"
$value->d['products_model_oem']
⇄products_similar => string (3) "N/A"
$value->d['products_similar']
⇄ncbi_gi_num => string (8) "61661420"
$value->d['ncbi_gi_num']
⇄ncbi_acc_num => string (10) "AAX51300.1"
$value->d['ncbi_acc_num']
⇄ncbi_acc_num_related => string (3) "N/A"
$value->d['ncbi_acc_num_related']
⇄ncbi_sp_acc_num_related => string (3) "N/A"
$value->d['ncbi_sp_acc_num_related']
⇄ncbi_gb_acc_num => string (3) "N/A"
$value->d['ncbi_gb_acc_num']
⇄sp_acc_num => string (3) "N/A"
$value->d['sp_acc_num']
⇄sp_acc_num_syn => string (3) "N/A"
$value->d['sp_acc_num_syn']
⇄products_image => string (9) "ELISA Kit"
$value->d['products_image']
⇄sequence_positions => string (3) "N/A"
$value->d['sequence_positions']
⇄sequence_length => string (3) "190"
$value->d['sequence_length']
⇄sequence => string (3) "N/A"
$value->d['sequence']
⇄clonality => string (3) "N/A"
$value->d['clonality']
⇄isotype => string (3) "N/A"
$value->d['isotype']
⇄clone_number => string (3) "N/A"
$value->d['clone_number']
⇄host => string (3) "N/A"
$value->d['host']
⇄reactivity => string (3) "Rat"
$value->d['reactivity']
⇄⧉specificity => string (135) "This kit recognizes Rat GLUT4 in samples. No significant cross-reactivity or...
$value->d['specificity']
This kit recognizes Rat GLUT4 in samples. No significant cross-reactivity or interference between Rat GLUT4 and analogues was observed.
⇄purity => string (3) "N/A"
$value->d['purity']
⇄form => string (3) "N/A"
$value->d['form']
⇄concentration => string (3) "N/A"
$value->d['concentration']
⇄storage_stability => string (20) "Store at 4 degree C."
⇄⧉etc_term1 => string (140) "Samples||Serum, plasma and other biological fluids!!Assay Type||Quantitative...
$value->d['etc_term1']
Samples||Serum, plasma and other biological fluids!!Assay Type||Quantitative Sandwich!!Detection Range||0.63-40ng/mL!!Sensitivity||0.38ng/mL
⇄⧉etc_term2 => string (370) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): 3 ...
$value->d['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat GLUT4 were tested 20 times on one plate, respectively.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat GLUT4 were tested on 3 different plates, 20 replicates in each plate.
⇄⧉products_description => string (1207) "Intended Uses: This ELISA kit applies to the in vitro quantitative determina...
$value->d['products_description']
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat GLUT4 concentrations in serum, plasma and other biological fluids.<br><br>Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GLUT4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GLUT4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GLUT4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat GLUT4. You can calculate the concentration of Rat GLUT4 in the samples by comparing the OD of the samples to the standard curve.
⇄products_references => string (3) "N/A"
$value->d['products_references']
⇄products_related_diseases => string (3) "N/A"
$value->d['products_related_diseases']
⇄products_categories => string (3) "N/A"
$value->d['products_categories']
⇄products_search_terms => string (3) "N/A"
$value->d['products_search_terms']
⇄ncbi_full_name => string (14) "glut4, partial"
$value->d['ncbi_full_name']
⇄ncbi_full_name_syn => string (3) "N/A"
$value->d['ncbi_full_name_syn']
⇄ncbi_symbol => string (3) "N/A"
$value->d['ncbi_symbol']
⇄ncbi_symbol_syn => string (3) "N/A"
$value->d['ncbi_symbol_syn']
⇄ncbi_protein_info => string (3) "N/A"
$value->d['ncbi_protein_info']
⇄ncbi_chrom_loc => string (3) "N/A"
$value->d['ncbi_chrom_loc']
⇄ncbi_gene_id => string (3) "N/A"
$value->d['ncbi_gene_id']
⇄ncbi_mol_weight => string (9) "20,643 Da"
$value->d['ncbi_mol_weight']
⇄ncbi_pathways => string (3) "N/A"
$value->d['ncbi_pathways']
⇄sp_protein_name => string (5) "Glut4"
$value->d['sp_protein_name']
⇄sp_protein_name_syn => string (5) "Glut4"
$value->d['sp_protein_name_syn']
⇄sp_gene_name => string (3) "N/A"
$value->d['sp_gene_name']
⇄sp_gene_name_syn => string (3) "N/A"
$value->d['sp_gene_name_syn']
⇄sp_entry_name => string (12) "Q58HK0_SHEEP"
$value->d['sp_entry_name']
⇄sp_mim => string (3) "N/A"
$value->d['sp_mim']
⇄sp_interactions => string (3) "N/A"
$value->d['sp_interactions']
⇄products_url => string (3) "N/A"
$value->d['products_url']
⇄products_viewed => string (1) "0"
$value->d['products_viewed']
⇄ncbi_summary_pdns => string (3) "N/A"
$value->d['ncbi_summary_pdns']
⇄sp_comments_pdns => string (3) "N/A"
$value->d['sp_comments_pdns']
⇄ncbi_research_articles_pdns => string (3) "N/A"
$value->d['ncbi_research_articles_pdns']
⇄products_description_extra => string (3) "N/A"
$value->d['products_description_extra']
⇄⧉public fields_below -> array (3)
$value->fields_below
⇄0 => string (31) "Related Product Information for"
⇄specificity => string (76) "Specifically recognize GLUT4, no obvious cross reaction with other analogues"
$value[0]['_source']['specificity']
⇄purity => string (3) "N/A"
$value[0]['_source']['purity']
⇄form => string (3) "N/A"
$value[0]['_source']['form']
⇄concentration => string (3) "N/A"
$value[0]['_source']['concentration']
⇄⧉storage_stability => string (156) "Store entire kit at 2-8C for short-term. For longer-term, please store the m...
$value[0]['_source']['storage_stability']
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Assay Type||Sandwich!!Samples||Serum, plasma, cell culture supernatant and other biological samples!!Detection Range||0.625-40ng/ml!!Sensitivity||0.375ng/ml
⇄⧉etc_term2 => string (270) "Intra-assay Precision||Intra-assay Precision: samples with low, medium and h...
$value[0]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.!!Inter-assay Precision||Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
GLUT4/SLC2A4/Solute carrier family 2; facilitated glucose transporter member 4/Glucose transporter type 4; insulin-responsive/GLUT-4/GLUT-4/insulin-responsive glucose transporter type 4
⇄products_gene_name => string (5) "GLUT4"
$value[0]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[0]['_source']['products_gene_name_syn']
⇄⧉products_description => string (1491) "Background: Glut4 (Glucose Transporter Member 4), also known as SLC2A4, is a...
$value[0]['_source']['products_description']
Background: Glut4 (Glucose Transporter Member 4), also known as SLC2A4, is a 509 aa multi-pass type membrane protein. It is an insulin-regulated glucose transporter. Glut4 is most highly expressed in adipose and striated muscle tissues, but has also been reported in multiple other tissues including the nervous system and breast cancer. Insulin stimulated transport of Glut4 has been shown to be impaired in type 2 diabetes patients. Additionally, inhibition of Glut4 may be useful in reducing proliferation of breast cancer cells.<br><br>Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti GLUT4 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti GLUT4 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with GLUT4 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of GLUT4 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
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⇄ncbi_pathways => string (3) "N/A"
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⇄sp_gene_name => string (3) "N/A"
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⇄⧉search_terms => string (640) "aaa17681 rat this assay has high sensitivity and excellent specificity for d...
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aaa17681 rat this assay has high sensitivity and excellent specificity for detection of glut4 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa17681_sc elisa kit glucose transporter 4 slc2a4 solute carrier family 2 facilitated member type insulin responsive glut samples mouse cell tissue homogenates sandwich range 0.156 10ng ml <0.094ng intra precision within an 3 with low middle level were tested 20 times on one plate respectively cv<8 inter assays different plates 8 replicates in each cv = sd meanx100 cv<10 transporter4 family2 an3 tested20 plates8
⇄⧉etc_term1 => string (140) "Samples||Serum, plasma and other biological fluids!!Assay Type||Quantitative...
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Samples||Serum, plasma and other biological fluids!!Assay Type||Quantitative Sandwich!!Detection Range||0.63-40ng/mL!!Sensitivity||0.38ng/mL
⇄⧉etc_term2 => string (370) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): 3 ...
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat GLUT4 were tested 20 times on one plate, respectively.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat GLUT4 were tested on 3 different plates, 20 replicates in each plate.
⇄⧉products_description => string (1207) "Intended Uses: This ELISA kit applies to the in vitro quantitative determina...
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Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat GLUT4 concentrations in serum, plasma and other biological fluids.<br><br>Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GLUT4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GLUT4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GLUT4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat GLUT4. You can calculate the concentration of Rat GLUT4 in the samples by comparing the OD of the samples to the standard curve.
⇄products_references => string (3) "N/A"
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⇄ncbi_full_name => string (14) "glut4, partial"
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⇄ncbi_symbol => string (3) "N/A"
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⇄ncbi_chrom_loc => string (3) "N/A"
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⇄ncbi_gene_id => string (3) "N/A"
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⇄ncbi_mol_weight => string (9) "20,643 Da"
$value[1]['_source']['ncbi_mol_weight']
⇄ncbi_pathways => string (3) "N/A"
$value[1]['_source']['ncbi_pathways']
⇄sp_protein_name => string (5) "Glut4"
$value[1]['_source']['sp_protein_name']
⇄sp_protein_name_syn => string (5) "Glut4"
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⇄sp_gene_name => string (3) "N/A"
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⇄sp_gene_name_syn => string (3) "N/A"
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⇄sp_entry_name => string (12) "Q58HK0_SHEEP"
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⇄sp_mim => string (3) "N/A"
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⇄⧉search_terms => string (567) "aaa21568 rat this kit recognizes glut4 in samples no significant cross react...
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aaa21568 rat this kit recognizes glut4 in samples no significant cross reactivity or interference between and analogues was observed typical testing data standard curve for reference only aaa21568_td elisa glucose transporter 4 partial 20,643 da q58hk0_sheep 61661420 aax51300.1 serum plasma other biological fluids assay type quantitative sandwich detection range 0.63 40ng ml sensitivity 0.38ng intra precision within an 3 with low mid high level were tested 20 times on one plate respectively inter assays different plates replicates each transporter4 an3 tested20
⇄ncbi_full_name => string (65) "Solute carrier family 2, facilitated glucose transporter member 4"
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⇄ncbi_full_name_syn => string (67) "solute carrier family 2 (facilitated glucose transporter), member 4"
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⇄ncbi_symbol => string (6) "SLC2A4"
$value[2]['_source']['ncbi_symbol']
⇄ncbi_symbol_syn => string (5) "GLUT4"
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⇄⧉ncbi_protein_info => string (168) "solute carrier family 2, facilitated glucose transporter member 4; GLUT-4; i...
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solute carrier family 2, facilitated glucose transporter member 4; GLUT-4; insulin-responsive glucose transporter type 4; glucose transporter type 4, insulin-responsive
Assay Type||Quantitative Sandwich!!Samples||Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids!!Detection Range||0.2-60ng/ml!!Sensitivity||0.11ng/ml
⇄⧉etc_term2 => string (403) "Intra-assay Precision||Intra-Assay Precision (Precision within an assay) Thr...
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Intra-assay Precision||Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%!!Inter-assay Precision||Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
⇄⧉products_description => string (887) "Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (EL...
$value[3]['_source']['products_description']
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat GLUT4 antibody. GLUT4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat GLUT4 Antibody is added and binds to GLUT4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GLUT4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat GLUT4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.<br><br>Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Glucose transporter 4 (also known as GLUT4) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
⇄products_references => string (3) "N/A"
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⇄products_related_diseases => string (3) "N/A"
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⇄products_categories => string (3) "N/A"
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⇄ncbi_full_name => string (14) "glut4, partial"
$value[3]['_source']['ncbi_full_name']
⇄ncbi_full_name_syn => string (3) "N/A"
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⇄ncbi_symbol => string (6) "SLC2A4"
$value[3]['_source']['ncbi_symbol']
⇄ncbi_symbol_syn => string (5) "GLUT4"
$value[3]['_source']['ncbi_symbol_syn']
⇄ncbi_protein_info => string (65) "solute carrier family 2, facilitated glucose transporter member 4"
⇄⧉search_terms => string (572) "aaa11117 rat typical testing data standard curve for reference only aaa11117...
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aaa11117 rat typical testing data standard curve for reference only aaa11117_sc elisa kit glucose transporter 4 glut4 partial slc2a4 solute carrier family 2 facilitated member 20,643 da 61661420 aax51300.1 samples serum plasma cell culture supernates ascites tissue homogenates or other biological fluids assay type quantitative sandwich detection range 0.2 60ng ml sensitivity 0.11ng intra precision within an three of known concentration were tested on one plate to assess cv<8 inter between assays in separate cv = sd mean x 100 cv<10 transporter4 family2 range0.2 x100
⇄⧉specificity => string (379) "This assay has high sensitivity and excellent specificity for detection of G...
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This assay has high sensitivity and excellent specificity for detection of GLUT4. No significant cross-reactivity or interference between GLUT4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GLUT4 and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
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⇄form => string (3) "N/A"
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⇄concentration => string (3) "N/A"
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⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄⧉products_description => string (1402) "Principle of the Assay: GLUT4 ELISA kit applies the competitive enzyme immun...
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Principle of the Assay: GLUT4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GLUT4 antibody and an GLUT4-HRP conjugate. The assay sample and buffer are incubated together with GLUT4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GLUT4 concentration since GLUT4 from samples and GLUT4-HRP conjugate compete for the anti-GLUT4 antibody binding site. Since the number of sites is limited, as more sites are occupied by GLUT4 from the sample, fewer sites are left to bind GLUT4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GLUT4 concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This GLUT4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse GLUT4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄⧉search_terms => string (614) "aaa16364 mouse this assay has high sensitivity and excellent specificity for...
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aaa16364 mouse this assay has high sensitivity and excellent specificity for detection of glut4 no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa16364_sc elisa kit glucose transporter 4 partial 20,643 da q58hk0_sheep 61661420 aax51300.1 signal transduction samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 0.1 ng ml transporter4 competitive0.1
⇄⧉storage_stability => string (196) "At -20 degree C for one year. After reconstitution, at 4 degree C for one mo...
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At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohistchemistry)||Anti-GLUT4 Picoband antibody, AAA11601-6.JPG<br>IHC(P): Mouse Skeletal Muscle Tissue||AAA11601_IHC6.jpg!!IHC (Immunohistochemistry)||Anti-GLUT4 Picoband antibody, AAA11601-5.JPG<br>IHC(P): Rat Cardiac Muscle Tissue||AAA11601_IHC5.jpg!!IHC (Immunohistochemistry)||Anti-GLUT4 Picoband antibody, AAA11601-4.JPG<br>IHC(P): Human Intestinal Cancer Tissue||AAA11601_IHC4.jpg!!IHC (Immunohistochemistry)||Anti-GLUT4 Picoband antibody, AAA11601-3.JPG<br>IHC(F): Mouse Cardiac Muscle Tissue||AAA11601_IHC3.jpg!!IHC (Immunohistochemistry)||Anti-GLUT4 Picoband antibody, AAA11601-2.JPG<br>IHC(F): Rat Cardiac Muscle Tissue||AAA11601_IHC2.jpg!!Application Data||Anti-GLUT4 Picoband antibody, AAA11601-1.jpg<br>All lanes: Anti GLUT4 (AAA11601) at 0.5ug/ml<br>Lane 1: Rat Cardiac MuscleTissue Lysate at 50ug<br>Lane 2: Rat Skeletal MuscleTissue Lysate at 50ug<br>Predicted bind size: 55KD<br>Observed bind size: 55KD||AAA11601_APP.jpg
⇄⧉etc_term1 => string (155) "Immunogen||E Coli-derived human GLUT4 recombinant protein (Position: N333-D5...
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Immunogen||E Coli-derived human GLUT4 recombinant protein (Position: N333-D509). Human GLUT4 shares 97% amino acid (aa) sequence identity with mouse GLUT4.
Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.!!Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
<b>Description: </b>Rabbit IgG polyclonal antibody for Solute carrier family 2, facilitated glucose transporter member 4(SLC2A4) detection. Tested with WB, IHC-P, IHC-F in Human, Mouse, Rat.<br><b>Background: </b>GLUT4, also known as SLC2A4 or solute carrier family 2 (facilitated glucose transporter) member 4, is a protein that in humans is encoded by the GLUT4 gene. It is mapped to 17p13.1. This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM).
⇄products_references => string (3) "N/A"
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⇄⧉products_related_diseases => string (243) "Insulin Resistance||844!!Hyperinsulinism||639!!Diabetes Mellitus, Type 2||29...
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Insulin Resistance||844!!Hyperinsulinism||639!!Diabetes Mellitus, Type 2||299!!Hyperglycemia||208!!Diabetes Mellitus, Experimental||182!!Cardiovascular Diseases||156!!Heart Diseases||114!!Necrosis||91!!Glucose Intolerance||85!!Inflammation||72
⇄products_categories => string (3) "N/A"
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⇄ncbi_full_name => string (65) "Solute carrier family 2, facilitated glucose transporter member 4"
$value[5]['_source']['ncbi_full_name']
⇄ncbi_full_name_syn => string (67) "solute carrier family 2 (facilitated glucose transporter), member 4"
$value[5]['_source']['ncbi_full_name_syn']
⇄ncbi_symbol => string (6) "SLC2A4"
$value[5]['_source']['ncbi_symbol']
⇄ncbi_symbol_syn => string (5) "GLUT4"
$value[5]['_source']['ncbi_symbol_syn']
⇄⧉ncbi_protein_info => string (168) "solute carrier family 2, facilitated glucose transporter member 4; GLUT-4; i...
$value[5]['_source']['ncbi_protein_info']
solute carrier family 2, facilitated glucose transporter member 4; GLUT-4; insulin-responsive glucose transporter type 4; glucose transporter type 4, insulin-responsive
⇄⧉search_terms => string (902) "aaa11601 rabbit human mouse rat polyclonal igg immunogen affinity purified l...
$value[5]['_source']['search_terms']
aaa11601 rabbit human mouse rat polyclonal igg immunogen affinity purified lyophilized western blot wb immunohistochemistry ihc paraffin formalin testing data anti glut4 picoband antibody 1 all lanes at 0.5ug ml lane cardiac muscletissue lysate 50ug 2 skeletal predicted bind size 55kd observed aaa11601_td 2.jpg f muscle tissue aaa11601_ihc2 3.jpg aaa11601_ihc3 4.jpg p intestinal cancer aaa11601_ihc4 5.jpg aaa11601_ihc5 6.jpg aaa11601_ihc6 solute carrier family facilitated glucose transporter member 4 insulin responsive glut type gtr4_human kug slc 2a4 slc2a4 43,760 da 121761 p14672.1 p14672 q05bq3 q14cx2 138190 e coli derived recombinant protein position n333 d509 shares 97 amino acid aa sequence identity with contents each vial contains 5mg bsa 0.9mg nacl 0.2mg na2hpo4 0.05mg nan3 reconstitution add 0.2ml of distilled water will yield a concentration 500ug antibody1 50ug2 member4 shares97
⇄⧉specificity => string (185) "This assay has high sensitivity and excellent specificity for detection of h...
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This assay has high sensitivity and excellent specificity for detection of human LRRC17. No significant cross-reactivity or interference between human LRRC17 and analogues was observed.
⇄purity => string (3) "N/A"
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⇄form => string (3) "N/A"
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⇄concentration => string (3) "N/A"
$value[6]['_source']['concentration']
⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (327) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Human Leucine-rich repeat-containing protein 17 (LRRC17) ELISA kit; P37NB; 37 kDa leucine-rich repeat (LRR) protein; leucine rich repeat containing 17
⇄products_gene_name => string (6) "LRRC17"
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⇄products_gene_name_syn => string (3) "N/A"
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⇄⧉products_description => string (739) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LRRC17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LRRC17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LRRC17 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LRRC17 bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄⧉search_terms => string (688) "aaa18114 human this assay has high sensitivity and excellent specificity for...
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aaa18114 human this assay has high sensitivity and excellent specificity for detection of lrrc17 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa18114_td elisa kit leucine rich repeat containing 17 protein p37nb 37 kda lrr isoform 1 51,800 da unq3076 pro9909 lrc17_human 72534676 np_001026862.1 q8n6y2 nm_001031692.2 q13288 q6uwa7 q75mg5 samples serum plasma tissue homogenates cell lysates type quantitative sandwich range 23.44 pg ml 1500 < 5.86 intra precision within an cv <8 three known concentration were tested twenty times on one plate to assess inter assays <10 in containing17 p37nb37 isoform1
<b>Storage:</b><br>Avoid repeated freeze/thaw cycles.<br>Store at 2-8 degree C for one month.<br>Aliquot and store at -80 degree C for 12 months.<br><br><b>Stability Test:</b><br>The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
⇄app_tested => string (57) "Positive Control; Immunogen; SDS-PAGE; Western Blot (WB)."
$value[7]['_source']['app_tested']
⇄app_notes => string (75) "(May be suitable for use in other assays to be determined by the end user.)"
⇄⧉etc_term2 => string (236) "Residues||Phe1479~Pro1729!!Tags||N-terminal His Tag!!Subcellular Location||M...
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Residues||Phe1479~Pro1729!!Tags||N-terminal His Tag!!Subcellular Location||Membrane, Mitochondrion, Cytoplasm!!Predicted isoelectric point||7.8!!Usage||Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
aaa20265 e.coli > 97 pbs ph7.4 containing 0.01 skl 1mm dtt 5 trehalose and proclin300 positive control immunogen sds page western blot wb sequence aaa20265_td aaa20265_sds recombinant protein leucine rich repeat kinase 2 lrrk2 serine threonine park8 ripk7 roco2 aura17 dardarin augmented in rheumatoid arthritis 17 predicted molecular mass 30.8kda accurate 30kda as determined by reducing conditions lrrk2_human 171846278 np_940980.3 q5s007 nm_198578.3 q6zs50 q8ncx9 a6nju2 168600 organism species homo sapiens human source prokaryotic expression residues phe1479~pro1729 tags n terminal his tag subcellular location membrane mitochondrion cytoplasm traits freeze dried powder isoelectric point 7.8 usage reconstitute 10mm to a concentration of 0.1 1.0 mg ml do not vortex >97 dtt5 kinase2 arthritis17 point7.8 of0.1
⇄⧉specificity => string (181) "This assay has high sensitivity and excellent specificity for detection of h...
$value[8]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of human LRG1. No significant cross-reactivity or interference between human LRG1 and analogues was observed.
⇄purity => string (3) "N/A"
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⇄form => string (3) "N/A"
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⇄concentration => string (3) "N/A"
$value[8]['_source']['concentration']
⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
$value[8]['_source']['storage_stability']
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (328) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<12%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
⇄⧉products_description => string (643) "Principle of the Assay: This assay employs the competitive inhibition enzyme...
$value[8]['_source']['products_description']
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are added to the appropriate microtiter plate wells with antibody specific for LRG1 and Horseradish Peroxidase (HRP) conjugated goat-anti-mouse antibody. The competitive inhibition reaction is launched between with pre-coated LRG1 and LRG1 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of LRG1 in the sample. The color development is stopped and the intensity of the color is measured.
⇄⧉search_terms => string (560) "aaa15188 human this assay has high sensitivity and excellent specificity for...
$value[8]['_source']['search_terms']
aaa15188 human this assay has high sensitivity and excellent specificity for detection of lrg1 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa15188_td elisa kit leucine rich alpha 2 glycoprotein 1 hmft1766 lrg 1300008b03rik 2310031e04rik 38,178 da a2gl_human 16418467 np_443204.1 p02750 nm_052972.2 q8n4f5 q96qz4 611289 samples serum urine tissue homogenates type quantitative competitive range 0.156 ug ml 40 < 0.156ug intra precision within an cv alpha2 glycoprotein1 ml40
Assay Type||Quantitative Sandwich!!Samples||Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids!!Detection Range||10-2000ng/L!!Sensitivity||4.49ng/L
⇄⧉etc_term2 => string (403) "Intra-assay Precision||Intra-Assay Precision (Precision within an assay) Thr...
$value[9]['_source']['etc_term2']
Intra-assay Precision||Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%!!Inter-assay Precision||Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Human Leucine-rich repeat-containing G-protein coupled receptor 5, LGR5 ELISA Kit
⇄products_name_syn => string (3) "N/A"
$value[9]['_source']['products_name_syn']
⇄products_gene_name => string (4) "LGR5"
$value[9]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[9]['_source']['products_gene_name_syn']
⇄⧉products_description => string (926) "Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (EL...
$value[9]['_source']['products_description']
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human LGR5 antibody. LGR5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human LGR5 Antibody is added and binds to LGR5 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated LGR5 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human LGR5. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.<br><br>Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Leucine-rich repeat-containing G-protein coupled receptor 5 (also known as LGR5) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
⇄⧉ncbi_pathways => string (186) "Signal Transduction Pathway||1262674!!Signaling By Wnt Pathway||1262900!!TCF...
$value[9]['_source']['ncbi_pathways']
Signal Transduction Pathway||1262674!!Signaling By Wnt Pathway||1262900!!TCF Dependent Signaling In Response To WNT Pathway||1262905!!Regulation Of FZD By Ubiquitination Pathway||1262912
⇄⧉search_terms => string (525) "aaa19074 human typical testing data standard curve for reference only aaa190...
$value[9]['_source']['search_terms']
aaa19074 human typical testing data standard curve for reference only aaa19074_sc elisa kit leucine rich repeat containing g protein coupled receptor 5 lgr5 gpr49 lgr5_rat 157817525 d4ac13 np_001100254.1 samples serum plasma cell culture supernates lysates tissue homogenates assay type quantitative sandwich detection range 10ng l 2000ng sensitivity 4.49ng intra precision within an three of known concentration were tested on one plate to assess cv<8 inter between assays in separate cv = sd mean x 100 cv<10 receptor5 x100
⇄⧉products_description => string (650) "Receptor for R-spondins that potentiates the canonical Wnt signaling pathway...
$value[10]['_source']['products_description']
Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a st cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal st cells during postembryonic development.
⇄⧉products_references => string (5855) "Identification and cloning of an orphan G protein-coupled receptor of the gl...
$value[10]['_source']['products_references']
Identification and cloning of an orphan G protein-coupled receptor of the glycoprotein hormone receptor subfamily.McDonald T., Wang R., Bailey W., Xie G., Chen F., Caskey C.T., Liu Q.Biochem. Biophys. Res. Commun. 247:266-270(1998)
Characterization of two LGR genes homologous to gonadotropin and thyrotropin receptors with extracellular leucine-rich repeats and a G protein-coupled, seven-transmembrane region.Hsu S.Y., Liang S.-G., Hsueh A.J.W.Mol. Endocrinol. 12:1830-1845(1998)
Alternatively spliced transcript of the GPR49-mRNA occur in different tumor cell lines and soft tissue sarcoma.Rot S., Taubert H., Bache M., Vordermark D., Kappler M.The finished DNA sequence of human chromosome 12.Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.Nature 440:346-351(2006)
Overexpression of orphan G-protein-coupled receptor, Gpr49, in human hepatocellular carcinomas with beta-catenin mutations.Yamamoto Y., Sakamoto M., Fujii G., Tsuiji H., Kenetaka K., Asaka M., Hirohashi S.Hepatology 37:528-533(2003)
Identification of overexpression of orphan G protein-coupled receptor GPR49 in human colon and ovarian primary tumors.McClanahan T., Koseoglu S., Smith K., Grein J., Gustafson E., Black S., Kirschmeier P., Samatar A.A.Cancer Biol. Ther. 5:419-426(2006)
Immunostaining of Lgr5, an intestinal stem cell marker, in normal and premalignant human gastrointestinal tissue.Becker L., Huang Q., Mashimo H.ScientificWorldJournal 8:1168-1176(2008)
LGR4 and LGR5 are R-spondin receptors mediating Wnt/beta-catenin and Wnt/PCP signalling.Glinka A., Dolde C., Kirsch N., Huang Y.L., Kazanskaya O., Ingelfinger D., Boutros M., Cruciat C.M., Niehrs C.EMBO Rep. 12:1055-1061(2011)
Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling.de Lau W., Barker N., Low T.Y., Koo B.K., Li V.S., Teunissen H., Kujala P., Haegebarth A., Peters P.J., van de Wetering M., Stange D.E., van Es J.E., Guardavaccaro D., Schasfoort R.B., Mohri Y., Nishimori K., Mohammed S., Heck A.J., Clevers H.Nature 476:293-297(2011)
R-spondins function as ligands of the orphan receptors LGR4 and LGR5 to regulate Wnt/beta-catenin signaling.Carmon K.S., Gong X., Lin Q., Thomas A., Liu Q.Proc. Natl. Acad. Sci. U.S.A. 108:11452-11457(2011)
R-Spondin potentiates Wnt/beta-catenin signaling through orphan receptors LGR4 and LGR5.Ruffner H., Sprunger J., Charlat O., Leighton-Davies J., Grosshans B., Salathe A., Zietzling S., Beck V., Therier M., Isken A., Xie Y., Zhang Y., Hao H., Shi X., Liu D., Song Q., Clay I., Hintzen G., Tchorz J., Bouchez L.C., Michaud G., Finan P., Myer V.E., Bouwmeester T., Porter J., Hild M., Bassilana F., Parker C.N., Cong F.PLoS ONE 7:E40976-E40976(2012)
Constitutive Internalization of the Leucine-rich G Protein-coupled Receptor-5 (LGR5)
to the Trans-Golgi Network.Snyder J.C., Rochelle L.K., Lyerly H.K., Caron M.G., Barak L.S.J. Biol. Chem. 288:10286-10297(2013)
Structure of stem cell growth factor R-spondin 1 in complex with the ectodomain of its receptor LGR5.Peng W.C., de Lau W., Forneris F., Granneman J.C., Huch M., Clevers H., Gros P.Cell Rep. 3:1885-1892(2013)
The structural basis of R-spondin recognition by LGR5 and RNF43.Chen P.H., Chen X., Lin Z., Fang D., He X.Genes Dev. 27:1345-1350(2013)
⇄⧉ncbi_pathways => string (186) "Regulation Of FZD By Ubiquitination Pathway||1269607!!Signal Transduction Pa...
$value[10]['_source']['ncbi_pathways']
Regulation Of FZD By Ubiquitination Pathway||1269607!!Signal Transduction Pathway||1269379!!Signaling By Wnt Pathway||1269594!!TCF Dependent Signaling In Response To WNT Pathway||1269599
⇄⧉search_terms => string (1532) "aaa18700 e coli or yeast baculovirus mammalian cell greater equal to 85 puri...
$value[10]['_source']['search_terms']
aaa18700 e coli or yeast baculovirus mammalian cell greater equal to 85 purity as determined by sds page lyophilized liquid format be during the manufacturing process aaa18700_sds gssprsgvllrgcpthchcepdgrmllrvdcsdlglselpsnlsvftsyldlsmnnisqllpnplpslrfleelrlagnaltyipkgaftglyslkvlmlqnnqlrhvptealqnlrslqslrldanhisyvppscfsglhslrhlwlddnalteipvqafrslsalqamtlalnkihhipdyafgnlsslvvlhlhnnrihslgkkcfdglhsletldlnynnldefptairtlsnlkelgfhsnnirsipekafvgnpslitihfydnpiqfvgrsafqhlpelrtltlngasqitefpdltgtanlesltltgaqisslpqtvcnqlpnlqvldlsynlledlpsfsvcqklqkidlrhneiyeikvdtfqqllslrslnlawnkiaiihpnafstlpslikldlssnllssfpitglhglthlkltgnhalqslissenfpelkviempyayqccafgvcenaykisnqwnkgdnssmddlhkkdagmfqaqderdledflldfeedlkalhsvqcspspgpfkpcehlldgwlir recombinant protein leucine rich repeat containing g coupled receptor 5 lgr5 human partial 49 67 hg38 isoform 2 fex gpr49 gpr67 grp49 62.4 kda lgr5_human 469608414 np_001264155.1 o75473 nm_001277226.1 q4vam0 q4vam2 q9up75 d8mct0 606667 cancer production note special offer host expressed is manufactured from a stock plasmid gene colihost stocked in different unit sizes ranging small 10 ug large 1 mg bulk inventory also available has been ordered over and again researchers stood test of time both robust important target for research community it part our new program make most popular targets corresponding hosts expanded with quick processing select fastest delivery among all please contact technical support team email [email protected] more details to85 receptor5 partial49 isoform2 small10 large1
⇄⧉products_description => string (405) "Functional component of the Nogo receptor signaling complex (RTN4R/NGFR) in ...
$value[11]['_source']['products_description']
Functional component of the Nogo receptor signaling complex (RTN4R/NGFR) in RhoA activation responsible for some inhibition of axonal regeneration by myelin-associated factors. Is also an important negative regulator of oligodentrocyte differentiation and axonal myelination. Acts in conjunction with RTN4 and RTN4R in regulating neuronal precursor cell motility during cortical development. 1 Publication
