Rabbit anti-Human, Mouse GRHL2 Polyclonal Antibody | anti-GRHL2 antibody

GRHL2 Antibody

Cat. Num. AAA9630067

• Gene Names: GRHL2; BOM; DFNA28; TFCP2L3
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: GRHL2; Polyclonal Antibody; GRHL2 Antibody; BOM; Brother of mammalian grainyhead; Deafness autosomal dominant 28; DFNA28; ECTDS; FLJ11172; FLJ13782; Grainyhead like 2 (Drosophila); Grainyhead like 2; Grainyhead like protein 2 homolog; Grainyhead like transcription factor 2; Grainyhead-like protein 2 homolog; GRHL 2; GRHL2_HUMAN; MGC149294; MGC149295; RGD1561191; TFCP2L3; Transcription factor CP2 like 3; Transcription factor CP2-like 3; anti-GRHL2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: GRHL2 Antibody detects endogenous levels of GRHL2.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-GRHL2 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human GRHL2.
• Conjugation: Unconjugated
• Similarity: Belongs to the grh/CP2 family. Grainyhead subfamily.
• Subunit Structure: Homodimer, also forms heterodimers with GRHL1 or GRHL3.
• Tissue Specificity: Expressed in keratinocytes (at protein level). Highly expressed in placenta, prostate, brain and kidney. Lower-level expression in a variety of epithelial tissues such as thymus, lung, salivary gland, mammary gland and digestive tract. Expressed in the cochlear. Expressed in corneal epithelial cells, but not in the endothelium or stroma.
• Function: Transcription factor playing an important role in primary neurulation and in epithelial development. Binds directly to the consensus DNA sequence 5'-AACCGGTT-3' acting as an activator and repressor on distinct target genes (By similarity). During embryogenesis, plays unique and cooperative roles with GRHL3 in establishing distinct zones of primary neurulation. Essential for closure 3 (rostral end of the forebrain), functions cooperatively with GRHL3 in closure 2 (forebrain/midbrain boundary) and posterior neuropore closure (By similarity). Regulates epithelial morphogenesis acting as a target gene-associated transcriptional activator of apical junctional complex components. Up-regulates of CLDN3 and CLDN4, as well as of RAB25, which increases the CLDN4 protein and its localization at tight junctions (By similarity). Comprises an essential component of the transcriptional machinery that establishes appropriate expression levels of CLDN4 and CDH1 in different types of epithelia. Exhibits functional redundancy with GRHL3 in epidermal morphogenetic events and epidermal wound repair (By similarity). In lung, forms a regulatory loop with NKX2-1 that coordinates lung epithelial cell morphogenesis and differentiation (By similarity). In keratinocytes, plays a role in telomerase activation during cellular proliferation, regulates TERT expression by binding to TERT promoter region and inhibiting DNA methylation at the 5'-CpG island, possibly by interfering with DNMT1 enzyme activity. In addition, impairs keratinocyte differentiation and epidermal function by inhibiting the expression of genes clustered at the epidermal differentiation complex (EDC) as well as GRHL1 and GRHL3 through epigenetic mechanisms.
• Note: detected at cell-cell contact areas.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-GRHL2 antibody

Nucleus; Membrane; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 170784817
• NCBI GeneID: 79977
• NCBI Accession #: NP_079191.2
• NCBI GenBank Nucleotide #: NM_024915.3
• UniProt Accession #: Q6ISB3; Q6NT03; Q9H8B8; A1L303
• Molecular Weight: 71,105 Da
• NCBI Official Full Name: grainyhead-like protein 2 homolog
• NCBI Official Synonym Full Names: grainyhead-like 2 (Drosophila)
• NCBI Official Symbol: GRHL2
• NCBI Official Synonym Symbols: BOM; DFNA28; TFCP2L3
• NCBI Protein Information: grainyhead-like protein 2 homolog; brother of mammalian grainyhead; transcription factor CP2-like 3
• UniProt Protein Name: Grainyhead-like protein 2 homolog
• Protein Family: Grainyhead-like protein
• UniProt Gene Name: GRHL2
• UniProt Synonym Gene Names: BOM; TFCP2L3
• UniProt Entry Name: GRHL2_HUMAN